Abstract: An electroporation system including one or more of a pipette, a pipette tip, a pipette docking assembly, and a pulse generator. The pipette docking assembly includes a pipette station, a pipette station guard, and a reservoir (e.g., a buffer tube). A method for transfecting a cell with a payload including providing an electroporation system, providing the cell, providing the payload, introducing the cell and the payload into a pipette tip, and electroporating the cell within the pipette tip by operating the electroporation system.

Abstract: A system for capturing CO2 gas comprising: a gaseous feed stream having an initial concentration of the CO2 gas; wherein the gaseous feed stream is provided to a first reactor as a gaseous reaction stream; the first reactor comprising a sorbent composition and the gaseous reaction stream flowing therein, the gaseous reaction stream being in contact with the sorbent composition; and a first gaseous output stream having a concentration of CO2 being less than the initial concentration of CO2; wherein: the gaseous reaction stream comprises the CO2 gas and is characterized by a relative humidity of at least 5%; the sorbent composition comprises a metal carbonate material that reacts with the CO2 gas of the gaseous reaction stream thereby reducing CO2 gas concentration; and the first reactor comprises 35 wt. % or less of liquid water by weight of sorbent and liquid water.

Abstract: The present disclosure provides a method and apparatus for operating an infrastructure layer of cloud computing architecture. A specific implementation of the method comprises: determining, in response to receiving an operation request sent by a user via a user terminal for the infrastructure layer, whether to execute an operation indicated by the operation request; sending, in response to determining to execute the operation, the operation request to the infrastructure layer; updating, after the infrastructure layer successfully executing the operation and updating the resources related to the infrastructure layer, a state of the resources related to the infrastructure layer stored in the intermediate layer database; acquiring changed data in the resources related to the infrastructure layer, and storing the data in the infrastructure layer database; and synchronizing the data in the infrastructure layer database to the intermediate layer database.

Abstract: The present disclosure provides a method and apparatus for operating an infrastructure layer of cloud computing architecture. A specific implementation of the method comprises: determining, in response to receiving an operation request sent by a user via a user terminal for the infrastructure layer, whether to execute an operation indicated by the operation request; sending, in response to determining to execute the operation, the operation request to the infrastructure layer; updating, after the infrastructure layer successfully executing the operation and updating the resources related to the infrastructure layer, a state of the resources related to the infrastructure layer stored in the intermediate layer database; acquiring changed data in the resources related to the infrastructure layer, and storing the data in the infrastructure layer database; and synchronizing the data in the infrastructure layer database to the intermediate layer database.