Abstract: The invention is directed to systems, apparatus and kits for automated synthesis of a plurality of polynucleotides in an array of reaction chambers using a template-free polymerase. In some embodiments, adaptive elements and processes are provided to monitor and control disruption of the synthesis process and fluid movement by enzyme aggregation.

Abstract: The invention is directed to methods and compositions for inkjet assisted synthesis of a plurality of polynucleotides at reaction sites on a substrate using template-free polymerases, such as, terminal deoxynucleotidyl transferases (TdTs). Compositions of the invention include formulations of synthesis reagents for inkjet delivery including, but not limited to, TdT coupling reaction buffers and 3?-O-protected dNTP monomers.

Abstract: The invention is directed to systems, apparatus and kits for automated synthesis of a plurality of polynucleotides in an array of reaction chambers using a template-free polymerase. In some embodiments, adaptive elements and processes are provided to monitor and control disruption of the synthesis process and fluid movement by enzyme aggregation.

Abstract: The present invention relates to a semi-active hydropneumatic suspension device which comprises a hydraulic cylinder associated with each of the wheels, provided with a main chamber connected through the medium of a damper with a main hydropneumatic accumulator, each hydraulic cylinder of the front axle being equipped with a height corrector, whereas the rear axle is equipped with a height corrector controlling at least one of the hydraulic cylinders of this axle, wherein each of the hydraulic cylinders is provided with an annular chamber hydraulically connected with at least one hydropneumatic accumulator of the suspension, whereas the height corrector of the rear axle is fed by an additional hydropneumatic compensating accumulator.