Abstract: In one embodiment, the present invention relates to a method for establishing a solvent correction curve as well as using the curve for obtaining a corrected sensorgram or corrected report points from a sensorgram of an analyte. In another embodiment, the present invention provides an analytical system for studying molecular interactions, which comprises computer processing means including program code means for performing the steps of the methods. Also provided is a computer program product comprising program code means stored on a computer readable medium or carried on an electrical or optical signal for performing the steps of the methods.

Abstract: A device and method for producing data for correction curves for SPR assays provides a plurality of different concentrations of solvent or other compound to the reference surface and active surface of a SPR chip. The concentration are mixed online by two pumps, one connected to a source of a first concentration of solvent or other compound and the other connected to a source of a second, different concentration of solvent or compound. Mixing of the two different concentration of solvent or compound can take place in a fluid mixer and the concentration of solvent or compound in the mixture leaving the fluid mixer can be varied by adjusting the relative speeds of the pumps. The concentration of the solvent or compound mixture can be adjusted during injection of the mixed liquids in order to provide the different concentrations necessary to make a solvent correction curve.

Abstract: In one embodiment, the present invention relates to a method for establishing a solvent correction curve as well as using the curve for obtaining a corrected sensorgram or corrected report points from a sensorgram of an analyte. In another embodiment, the present invention provides an analytical system for studying molecular interactions, which comprises computer processing means including program code means for performing the steps of the methods. Also provided is a computer program product comprising program code means stored on a computer readable medium or carried on an electrical or optical signal for performing the steps of the methods.

Abstract: A method of determining the total concentration of an analyte in a fluid sample, wherein at least part of the analyte is present as a complex with an analyte-binding species. The methods includes the steps of: a) subjecting the sample to conditions that reduce the binding affinity between analyte and analyte-binding species sufficiently to dissociate substantially any analyte complex and provide substantially all analyte in free form, b) subjecting the sample to conditions that restore the binding affinity between analyte and analyte-binding species, and c) immediately after the binding affinity has been restored, and before any substantial re-complexing of the analyte has taken place, determining the concentration of free analyte in the sample. A method of determining the concentration of complex-bound analyte in a sample is also disclosed.

Abstract: A method of determining the total concentration of an analyte in a fluid sample, wherein at least part of the analyte is present as a complex with an analyte-binding species. The methods includes the steps of: a) subjecting the sample to conditions that reduce the binding affinity between analyte and analyte-binding species sufficiently to dissociate substantially any analyte complex and provide substantially all analyte in free form, b) subjecting the sample to conditions that restore the binding affinity between analyte and analyte-binding species, and c) immediately after the binding affinity has been restored, and before any substantial re-complexing of the analyte has taken place, determining the concentration of free analyte in the sample. A method of determining the concentration of complex-bound analyte in a sample is also disclosed.