Abstract: The present invention provide scalable methods of producing newly identified atrorosin pigments, compositions comprising the pigments, lakes and dyes and uses thereof for colorings foods, such as dairy, meat substitutes or candy.

Abstract: A method for providing a hybrid polypeptide having an activity of interest, by i) performing PCR amplification using an uncharacterized DNA sample and oligonucleotide primers with homology to one or more known genes encoding a polypeptide exhibiting said activity of interest, to obtain one or more PCR products, ii) linking the obtained PCR products to a 5′ structural gene sequence and a 3′ structural gene sequence, wherein the 5′ and 3′ structural gene sequences are derived from one or more genes encoding a polypeptide exhibiting said activity of interest, to form hybrid DNA sequences, iii) expressing the resulting hybrid DNA sequences, and iv) screening the hybrid polypeptides to identify a sequence encoding a polypeptide exhibiting said activity of interest or a related activity.

Abstract: Novel yeast promoters for either EF1-alpha protein or ribosomal protein S7 gene suitable for expression cloning in yeast and heterologous expression of proteins in yeast. The yeast promoters are preferably active in the pH range 4-11 without peptone and obtained from the yeast strain Yarrowia lipolytica.

Abstract: An enzyme preparation comprising (a) first enzyme with xylanase activity and (b) a second enzyme having cellulotytic, xylanolytic or pectinolytic activity and are supported by the specification.

Abstract: An enzyme from Aspergillus aculeatus exhibiting endoglucanase activity encoded by the DNA sequence of SEQ ID NO:17 or 18, and useful for degrading plant cell walls.

Abstract: A DNA construct encoding an enzyme(s) exhibiting xylanase activity, which enzyme is immunologically reactive with antibody raised against a purified xylanase derived from Aspergillus aculealus, CBS 101.43, recombinant vectors and cells comprising said construct and a method for producing said enzyme using said cell comprising said construct.

Abstract: An isolated and purified enzyme exhibiting protease activity at a pH of 4-7 which exhibits protease in 5% hydrogen peroxide and which is encoded by a DNA sequence which hybridizes to a DNA sequence depicted in SEQ ID NO: 1 or 2. Methods are described for using the protease compositions in reducing vescosity, cleaning contact lenses, baking, and preparing animal feed.

Abstract: An enzyme exhibiting polygalacturonase activity, which enzyme is immunologically reactive with an antibody raised against a purified polygalacturonase derived from Aspergillus aculeatus, CBS 101.43. The enzyme may be produced by recombinant DNA techniques and may be used for degradation of plant cell walls, for instance in the wine and juice production.

Abstract: An enzyme exhibiting mannanase activity, which enzyme i) is immunologically reactive with an antibody raised against a purified mannanase derived from Aspergillus aculeatus, CBS 101.43; ii) is encoded by the DNA sequences shown in SEQ ID No. 1 or an analogue of said sequence, and/or; iii) comprises the amino acid sequence shown in SEQ ID No. 2 or a sequence being an least 70% homologous thereto. The enzyme may be used for various purposes for which degradation or modification of a plant or algal cell wall material is desirable.

Abstract: An enzyme from Aspergillus aculeatus exhibiting endoglucanase activity, which enzyme is designated EG II or EG IV and is encoded by a DNA sequence comprising at least one of the partial sequences shown in SEQ ID NO: 17, 18, or 19. The enzyme may be produced by recombinant DNA techniques and may be used for degradation of plant cell wall material.