Abstract: A system for time-resolved fluoroimmunoassay detection is disclosed. The system comprises a receptacle with a sample volume adapted for receiving a sample therein; a light source adapted to emit pulsed excitation light illumination optics adapted to collect the pulsed excitation light from the light source and to deliver the pulsed excitation light to the sample volume in the receptacle; a detection device adapted for gated detection of fluorescence radiation at least in a detection spectral range; detection optics adapted to collect fluorescence radiation from the receptacle at least in the detection spectral range and deliver the fluorescence radiation to the detection device; and an optical filter device configured for the separation of excitation light and detection light. A method for time-resolved fluoroimmunoassay detection is also provided.

Abstract: A system (100) for time-resolved fluoroimmunoassay detection is disclosed. The system (100) comprises a receptacle (110) with a sample volume adapted for receiving a sample (99) therein; a light source (120) adapted to emit pulsed excitation light (200); illumination optics (130) adapted to collect the pulsed excitation light (200) from the light source (120) and to deliver said pulsed excitation light (200) to the sample volume in the receptacle (110); a detection device (140) adapted for gated detection of fluorescence radiation (210) at least in a detection spectral range; detection optics (150) adapted to collect fluorescence radiation (210) from the receptacle (110) at least in the detection spectral range and deliver said fluorescence radiation (210) to the detection device (140); and an optical filter device (160) configured for the separation of excitation light (200) and detection light (210).

Abstract: An apparatus, a sample cuvette and a method for optical measurements on samples which show variable levels of scattering are provided. The apparatus, sample cuvette and method may be used for optical measurements on biological, physiological and chemical samples, however, are especially applicable for optical measurements on whole blood.

Abstract: An apparatus, a sample cuvette and a method for optical measurements on samples which show variable levels of scattering are provided. The apparatus, sample cuvette and method may be used for optical measurements on biological, physiological and chemical samples, however, are especially applicable for optical measurements on whole blood.

Abstract: A method and an apparatus for determining the content of a constituent of blood of an individual are disclosed. In the method a whole blood stream is extracted from a blood vessel of said individual, the stream being directed through a path defining a substantially non-varying flow-through area wherein is provided a flow-through measuring cuvette including opposite first and second optical transparent surface parts defining an optical transmission path of the order of 0.5-2.0 mm. The whole blood stream flowing through the measuring cuvette is irradiated by irradiating the first optical transparent surface part of the measuring cuvette with multi-wavelength near infrared light. The near infrared absorption spectrum is detected and the content of the constituent is quantified on the basis of the detected near infrared absorption data. The method is particularly suited for measuring constituents of whole blood in an extracorporeal loop, for example in hemodialysis.