Patents by Inventor Henry A. Graham
Henry A. Graham has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10107811Abstract: Methods for detecting the presence or absence of, and for quantifying, one set of cells in a mixed cell population of at least two sets of cells especially Rh positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH). The magnetic particles coated with anti-D antibodies are reacted with the Rh positive fetal cells in Rh negative maternal blood followed by a specific separation and quantifying technique. Gravitational forces or magnetic forces are used to move reacted magnetic particles to isolate, distinguish and quantify cells differentiated by antigenic composition. Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.Type: GrantFiled: November 4, 2015Date of Patent: October 23, 2018Assignee: Chrome Red Technologies, LLCInventors: Henry A. Graham, John G. Gorman, James P. Rowell
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Patent number: 9766256Abstract: Methods for separating, cells, particles, or other molecules of interest (MOI) from unwanted materials not of interest (MNOI). by forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, unwanted less dense materials from entering. Buoyant or other forces are used to remove any unbound material from the complexes with ASG before reading. Readable labels include the magnetic particle tagged complex itself, and other labels such as enzymes, chemiluminescent materials, radioactive isotopes, chromogenic and fluorogenic substrates and other labels may be used. The invention applies to many assays, diagnostic tests, separative procedures and chemical syntheses.Type: GrantFiled: September 22, 2016Date of Patent: September 19, 2017Assignee: CHROME RED TECHNOLOGIES, LLCInventors: Henry A. Graham, John G. Gorman, James P. Rowell
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Publication number: 20170016922Abstract: Methods for separating, in a continuous, multizone fluid medium, cells, particles, or other molecules of interest (MOI) from associated or contaminating unwanted materials not of interest (MNOI). The invention involves forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. Differential movement of MOI and MNOI occurs by active counterforces that move MNOI but not MOI. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, by flotation counterforce, unwanted less dense materials from entering. Surfaces specifically coated with reactants are reactive with the MOI in the tagged magnetic particle complex and of buoyant or other forces are used to remove any unbound material from the surface before reading.Type: ApplicationFiled: September 22, 2016Publication date: January 19, 2017Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
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Patent number: 9518984Abstract: Method for detecting a material of interest, usually an antibody or an antigen on a red blood cell, in a liquid, by observing the potential antigen/antibody reaction products in a novel way. Reaction products are deposited on a magnetic substrate and will exhibit different properties depending upon whether or not the antigen/antibody reaction has taken place. An antigen/antibody reaction product adheres tenaciously to itself and the substrate while the deposit of any unreacted magnetically tagged material is weakly adhering and easily disrupted and dislodged under a disruptive force which would be insufficient to dislodge the reacted deposit. This difference in cohesive property provides the means by which a positive result is distinguished from a negative result.Type: GrantFiled: February 21, 2012Date of Patent: December 13, 2016Assignee: CHROME RED TECHNOLOGIES, LLCInventors: Henry A. Graham, John G. Gorman, James P. Rowell
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Patent number: 9488665Abstract: Methods for separating, in a continuous, multizone fluid medium, cells, particles, or other molecules of interest (MOI) from associated or contaminating unwanted materials not of interest (MNOI). The invention involves forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. Differential movement of MOI and MNOI occurs by active counterforces that move MNOI but not MOI. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, by flotation counterforce, unwanted less dense materials from entering. Surfaces specifically coated with reactants are reactive with the MOI in the tagged magnetic particle complex and of buoyant or other forces are used to remove any unbound material from the surface before reading.Type: GrantFiled: March 8, 2007Date of Patent: November 8, 2016Assignee: CHROME RED TECHNOLOGIES, LLCInventors: Henry A. Graham, John G. Gorman, James P. Rowell
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Publication number: 20160069877Abstract: Methods for detecting the presence or absence of, and for quantifying, one set of cells in a mixed cell population of at least two sets of cells especially Rh positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH). The magnetic particles coated with anti-D antibodies are reacted with the Rh positive fetal cells in Rh negative maternal blood followed by a specific separation and quantifying technique. Gravitational forces or magnetic forces are used to move reacted magnetic particles to isolate, distinguish and quantify cells differentiated by antigenic composition. Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.Type: ApplicationFiled: November 4, 2015Publication date: March 10, 2016Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
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Patent number: 9182389Abstract: Detection of the presence or absence of, RH positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH). The novel methods utilize gravitational forces or applying magnetic forces to reactive magnetic particles to isolate, distinguish and quantify cells. Particles aggregate and are pulled or settle by gravity through a transparent separating solution into a measuring zone where the volume of Rh positive cells can be measured. Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.Type: GrantFiled: February 21, 2012Date of Patent: November 10, 2015Assignee: Chrome Red Technologies, LLCInventors: Henry A. Graham, John G. Gorman, James P. Rowell
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Publication number: 20120214176Abstract: Methods for detecting the presence or absence of, and for quantifying, one set of cells in a mixed cell population of at least two sets of cells especially Rh positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH). The magnetic particles coated with anti-D antibodies are reacted with the Rh positive fetal cells in Rh negative maternal blood followed by a specific separation and quantifying technique. Gravitational forces or magnetic forces are used to move reacted magnetic particles to isolate, distinguish and quantify cells differentiated by antigenic composition. Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.Type: ApplicationFiled: February 21, 2012Publication date: August 23, 2012Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
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Publication number: 20120214175Abstract: Method for detecting a material of interest, usually an antibody or an antigen on a red blood cell, in a liquid, by observing the potential antigen/antibody reaction products in a novel way. Reaction products are deposited on a magnetic substrate and will exhibit different properties depending upon whether or not the antigen/antibody reaction has taken place. An antigen/antibody reaction product adheres tenaciously to itself and the substrate while the deposit of any unreacted magnetically tagged material is weakly adhering and easily disrupted and dislodged under a disruptive force which would be insufficient to dislodge the reacted deposit. This difference in cohesive property provides the means by which a positive result is distinguished from a negative result.Type: ApplicationFiled: February 21, 2012Publication date: August 23, 2012Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
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Patent number: 5571726Abstract: A method and test kit useful in a wide variety of immunoassay systems. A method of producing metal sol reagents containing metal sol particles of a preselected size is provided. A metal containing solution is reduced under optimized pH conditions to produce metal sol particles of a preselected size, the particles are coated with a coupling compound and then bound with at least one selected immunochemically reactive component. Particles having different immunochemical specificities are also mixed to produce reagents having multiple selected immunochemical specificities.Type: GrantFiled: May 19, 1995Date of Patent: November 5, 1996Assignee: Ortho Diagnostic Systems, Inc.Inventors: Houston G. Brooks, Jr., Chi-Deu Chang, Utpal R. Chakraborty, Henry A. Graham, Jr., Lloyd L. Hollenbeck, Jr., Sharon R. Lawler, Jennifer Nasser, Ernest G. Schutt, Albert Venturini
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Patent number: 5514602Abstract: A method and test kit useful in a wide variety of immunoassay systems. A method of producing metal sol reagents containing metal sol particles of a preselected size is provided. A metal containing solution is reduced under optimized pH conditions to produce metal sol particles of a preselected size, the particles are coated with a coupling compound and then bound with at least one selected immunochemically reactive component. Particles having different immunochemical specificities are also mixed to produce reagents having multiple selected immunochemical specificities.Type: GrantFiled: February 25, 1993Date of Patent: May 7, 1996Assignee: Ortho Diagnostic Systems, Inc.Inventors: Houston G. Brooks, Jr., Chi-Deu Chang, Utpal R. Chakraborty, Henry A. Graham, Jr., Lloyd L. Hollenbeck, Jr., Sharon R. Lawler, Jennifer Nasser, Ernest G. Schutt, Albert Venturini
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Patent number: 4743542Abstract: A method is provided for substantially increasing the dynamic range of an immunoassay and forestalling the hook effect observed with very large ligand concentrations in aqueous samples. The method comprises the addition of ligand binding partners which do not have labels associated therewith to compete with labeled ligand binding partners thereby effectively eliminating the presence of the excess ligand in the sample solution. The conversant method for ligand binding partner immunoassays is also provided.Type: GrantFiled: April 11, 1985Date of Patent: May 10, 1988Assignee: Ortho DiagnosticInventors: Henry A. Graham, Jr., Peter Lisi
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Patent number: 4436631Abstract: A particle washing system and method of use is described wherein in a preferred embodiment the fluid containing the desired particles is placed within an inner tube having near the bottom thereof an orifice with a diameter at least equal to that of the diameter of the particles, and wherein the inner tube is positioned within an outer tube having a fluid with a density at least equal to that of the solution containing the particles to be separated but less than that of the particles. The application of centrifugal force to the particles directed toward the bottom of the outer tube causes the particles to move through the orifice and through the outer solution contained within the outer tube so that the particles are collected from the inner solution, washed by the outer solution, and subsequently sedimented at the bottom of the outer tube.Type: GrantFiled: September 29, 1982Date of Patent: March 13, 1984Assignee: Ortho Diagnostic Systems Inc.Inventors: Henry A. Graham, Jr., Johnna B. Hawk, Rosemary K. Chachowski
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Patent number: 4435293Abstract: A particle washing system and method of use is described wherein in a preferred embodiment the fluid containing the desired particles is placed within an inner tube having near the bottom thereof an orifice with a diameter at least equal to that of the diameter of the particles, an air vent and, wherein the inner tube is positioned within an outer tube having a fluid with a density at least equal to that of the solution containing the particles to be separated within the inner tube but less than that of the particles. The application of centrifugal force to the particles directed toward the bottom of the outer tube causes the particles to move through orifice and through the outer solution contained within the outer tube so that the particles are collected from the inner solution, washed by the outer solution, and subsequently sedimented at the bottom of the outer tube.Type: GrantFiled: August 5, 1981Date of Patent: March 6, 1984Assignee: Ortho Diagnostic Systems Inc.Inventors: Henry A. Graham, Jr., Johnna B. Hawk, Rosemary K. Chachowski
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Patent number: 4433059Abstract: Reagents and methods are described for an immunoassay test of increased sensitivity and decreased complexity employing an immunoglobulin specific for an antigen naturally or artificially placed upon the surface of an indicator particle coupled through the use of a hetero-bifunctional coupling reagent to a second antibody of differing specificity and specific for the antigen to be detected. In a preferred embodiment, a hetero-bifunctional coupling agent couples via a sulfhydryl group, a univalent immunoglobulin specific for the surface antigens on erythrocytes to a second multivalent immunoglobulin through an amide linkage with the latter immunoglobulin wherein said second immunoglobulin is specific for hepatitis-B surface antigen.Type: GrantFiled: September 8, 1981Date of Patent: February 21, 1984Assignee: Ortho Diagnostic Systems Inc.Inventors: Chi-Deu Chang, Henry A. Graham, Jr.
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Patent number: 4358436Abstract: Methods and reagents for determination of Rh.sub.o (D), C, c, E, e, and K antigens in human blood. The reagents are low-protein compositions containing reduced and S-alkylated IgG antibody to the selected antigen maintained at a pH of between about 7.5 and 8.3. These reagents meet or exceed FDA standards for potency and specificity of the respective antisera.Type: GrantFiled: June 9, 1980Date of Patent: November 9, 1982Assignee: Ortho Diagnostics, Inc.Inventors: Henry A. Graham, Jr., David J. Olekna, Johnna B. Hawk, Diane B. Kebles
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Patent number: 4296090Abstract: Methods and reagents for determination of Rh (D) antigen in human blood. The reagent is a low-protein composition containing reduced and S-alkylated anti-D IgG maintained at pH of between about 7.5 and 8.3. This reagent meets or exceeds FDA standards for potency of anti-D for the slide test of rapid tube test.Type: GrantFiled: October 5, 1979Date of Patent: October 20, 1981Assignee: Ortho Diagnostics, Inc.Inventors: Henry A. Graham, Jr., David J. Olekna, Johnna B. Hawk, Diane M. Barrett
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Patent number: D278653Type: GrantFiled: August 17, 1982Date of Patent: April 30, 1985Assignee: Ortho Diagnostic Systems Inc.Inventors: Steven R. Savitz, Johnna B. Hawk, Henry A. Graham, Jr., Rosemary K. Chachowski
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Patent number: D310265Type: GrantFiled: September 21, 1987Date of Patent: August 28, 1990Assignee: Ortho Diagnostic Systems Inc.Inventors: Dennis M. Mochnal, Henry A. Graham, Jr., Walter J. Yarrow, Jr., C. Minot Dole, Joseph R. Paradis