Patents by Inventor Henry A. Graham

Henry A. Graham has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 10107811
    Abstract: Methods for detecting the presence or absence of, and for quantifying, one set of cells in a mixed cell population of at least two sets of cells especially Rh positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH). The magnetic particles coated with anti-D antibodies are reacted with the Rh positive fetal cells in Rh negative maternal blood followed by a specific separation and quantifying technique. Gravitational forces or magnetic forces are used to move reacted magnetic particles to isolate, distinguish and quantify cells differentiated by antigenic composition. Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.
    Type: Grant
    Filed: November 4, 2015
    Date of Patent: October 23, 2018
    Assignee: Chrome Red Technologies, LLC
    Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
  • Patent number: 9766256
    Abstract: Methods for separating, cells, particles, or other molecules of interest (MOI) from unwanted materials not of interest (MNOI). by forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, unwanted less dense materials from entering. Buoyant or other forces are used to remove any unbound material from the complexes with ASG before reading. Readable labels include the magnetic particle tagged complex itself, and other labels such as enzymes, chemiluminescent materials, radioactive isotopes, chromogenic and fluorogenic substrates and other labels may be used. The invention applies to many assays, diagnostic tests, separative procedures and chemical syntheses.
    Type: Grant
    Filed: September 22, 2016
    Date of Patent: September 19, 2017
    Assignee: CHROME RED TECHNOLOGIES, LLC
    Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
  • Publication number: 20170016922
    Abstract: Methods for separating, in a continuous, multizone fluid medium, cells, particles, or other molecules of interest (MOI) from associated or contaminating unwanted materials not of interest (MNOI). The invention involves forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. Differential movement of MOI and MNOI occurs by active counterforces that move MNOI but not MOI. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, by flotation counterforce, unwanted less dense materials from entering. Surfaces specifically coated with reactants are reactive with the MOI in the tagged magnetic particle complex and of buoyant or other forces are used to remove any unbound material from the surface before reading.
    Type: Application
    Filed: September 22, 2016
    Publication date: January 19, 2017
    Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
  • Patent number: 9518984
    Abstract: Method for detecting a material of interest, usually an antibody or an antigen on a red blood cell, in a liquid, by observing the potential antigen/antibody reaction products in a novel way. Reaction products are deposited on a magnetic substrate and will exhibit different properties depending upon whether or not the antigen/antibody reaction has taken place. An antigen/antibody reaction product adheres tenaciously to itself and the substrate while the deposit of any unreacted magnetically tagged material is weakly adhering and easily disrupted and dislodged under a disruptive force which would be insufficient to dislodge the reacted deposit. This difference in cohesive property provides the means by which a positive result is distinguished from a negative result.
    Type: Grant
    Filed: February 21, 2012
    Date of Patent: December 13, 2016
    Assignee: CHROME RED TECHNOLOGIES, LLC
    Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
  • Patent number: 9488665
    Abstract: Methods for separating, in a continuous, multizone fluid medium, cells, particles, or other molecules of interest (MOI) from associated or contaminating unwanted materials not of interest (MNOI). The invention involves forced movement of MOI into certain zones having properties which deter the entry of unwanted materials. Differential movement of MOI and MNOI occurs by active counterforces that move MNOI but not MOI. MOI are tagged with magnetic particles and moved with a magnetic field through a fluid, or zones, of higher specific gravity that prevents, by flotation counterforce, unwanted less dense materials from entering. Surfaces specifically coated with reactants are reactive with the MOI in the tagged magnetic particle complex and of buoyant or other forces are used to remove any unbound material from the surface before reading.
    Type: Grant
    Filed: March 8, 2007
    Date of Patent: November 8, 2016
    Assignee: CHROME RED TECHNOLOGIES, LLC
    Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
  • Publication number: 20160069877
    Abstract: Methods for detecting the presence or absence of, and for quantifying, one set of cells in a mixed cell population of at least two sets of cells especially Rh positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH). The magnetic particles coated with anti-D antibodies are reacted with the Rh positive fetal cells in Rh negative maternal blood followed by a specific separation and quantifying technique. Gravitational forces or magnetic forces are used to move reacted magnetic particles to isolate, distinguish and quantify cells differentiated by antigenic composition. Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.
    Type: Application
    Filed: November 4, 2015
    Publication date: March 10, 2016
    Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
  • Patent number: 9182389
    Abstract: Detection of the presence or absence of, RH positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH). The novel methods utilize gravitational forces or applying magnetic forces to reactive magnetic particles to isolate, distinguish and quantify cells. Particles aggregate and are pulled or settle by gravity through a transparent separating solution into a measuring zone where the volume of Rh positive cells can be measured. Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.
    Type: Grant
    Filed: February 21, 2012
    Date of Patent: November 10, 2015
    Assignee: Chrome Red Technologies, LLC
    Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
  • Publication number: 20120214176
    Abstract: Methods for detecting the presence or absence of, and for quantifying, one set of cells in a mixed cell population of at least two sets of cells especially Rh positive cells in a mixed population with Rh negative cells, as is found in a fetal maternal hemorrhage (FMH). The magnetic particles coated with anti-D antibodies are reacted with the Rh positive fetal cells in Rh negative maternal blood followed by a specific separation and quantifying technique. Gravitational forces or magnetic forces are used to move reacted magnetic particles to isolate, distinguish and quantify cells differentiated by antigenic composition. Rh positive cell volume is correlated to the volume of the original blood sample as an indication of the number of doses of RhIG needed to be administered to the mother to prevent subsequent Rh immunization.
    Type: Application
    Filed: February 21, 2012
    Publication date: August 23, 2012
    Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
  • Publication number: 20120214175
    Abstract: Method for detecting a material of interest, usually an antibody or an antigen on a red blood cell, in a liquid, by observing the potential antigen/antibody reaction products in a novel way. Reaction products are deposited on a magnetic substrate and will exhibit different properties depending upon whether or not the antigen/antibody reaction has taken place. An antigen/antibody reaction product adheres tenaciously to itself and the substrate while the deposit of any unreacted magnetically tagged material is weakly adhering and easily disrupted and dislodged under a disruptive force which would be insufficient to dislodge the reacted deposit. This difference in cohesive property provides the means by which a positive result is distinguished from a negative result.
    Type: Application
    Filed: February 21, 2012
    Publication date: August 23, 2012
    Inventors: Henry A. Graham, John G. Gorman, James P. Rowell
  • Patent number: 5571726
    Abstract: A method and test kit useful in a wide variety of immunoassay systems. A method of producing metal sol reagents containing metal sol particles of a preselected size is provided. A metal containing solution is reduced under optimized pH conditions to produce metal sol particles of a preselected size, the particles are coated with a coupling compound and then bound with at least one selected immunochemically reactive component. Particles having different immunochemical specificities are also mixed to produce reagents having multiple selected immunochemical specificities.
    Type: Grant
    Filed: May 19, 1995
    Date of Patent: November 5, 1996
    Assignee: Ortho Diagnostic Systems, Inc.
    Inventors: Houston G. Brooks, Jr., Chi-Deu Chang, Utpal R. Chakraborty, Henry A. Graham, Jr., Lloyd L. Hollenbeck, Jr., Sharon R. Lawler, Jennifer Nasser, Ernest G. Schutt, Albert Venturini
  • Patent number: 5514602
    Abstract: A method and test kit useful in a wide variety of immunoassay systems. A method of producing metal sol reagents containing metal sol particles of a preselected size is provided. A metal containing solution is reduced under optimized pH conditions to produce metal sol particles of a preselected size, the particles are coated with a coupling compound and then bound with at least one selected immunochemically reactive component. Particles having different immunochemical specificities are also mixed to produce reagents having multiple selected immunochemical specificities.
    Type: Grant
    Filed: February 25, 1993
    Date of Patent: May 7, 1996
    Assignee: Ortho Diagnostic Systems, Inc.
    Inventors: Houston G. Brooks, Jr., Chi-Deu Chang, Utpal R. Chakraborty, Henry A. Graham, Jr., Lloyd L. Hollenbeck, Jr., Sharon R. Lawler, Jennifer Nasser, Ernest G. Schutt, Albert Venturini
  • Patent number: 4743542
    Abstract: A method is provided for substantially increasing the dynamic range of an immunoassay and forestalling the hook effect observed with very large ligand concentrations in aqueous samples. The method comprises the addition of ligand binding partners which do not have labels associated therewith to compete with labeled ligand binding partners thereby effectively eliminating the presence of the excess ligand in the sample solution. The conversant method for ligand binding partner immunoassays is also provided.
    Type: Grant
    Filed: April 11, 1985
    Date of Patent: May 10, 1988
    Assignee: Ortho Diagnostic
    Inventors: Henry A. Graham, Jr., Peter Lisi
  • Patent number: 4436631
    Abstract: A particle washing system and method of use is described wherein in a preferred embodiment the fluid containing the desired particles is placed within an inner tube having near the bottom thereof an orifice with a diameter at least equal to that of the diameter of the particles, and wherein the inner tube is positioned within an outer tube having a fluid with a density at least equal to that of the solution containing the particles to be separated but less than that of the particles. The application of centrifugal force to the particles directed toward the bottom of the outer tube causes the particles to move through the orifice and through the outer solution contained within the outer tube so that the particles are collected from the inner solution, washed by the outer solution, and subsequently sedimented at the bottom of the outer tube.
    Type: Grant
    Filed: September 29, 1982
    Date of Patent: March 13, 1984
    Assignee: Ortho Diagnostic Systems Inc.
    Inventors: Henry A. Graham, Jr., Johnna B. Hawk, Rosemary K. Chachowski
  • Patent number: 4435293
    Abstract: A particle washing system and method of use is described wherein in a preferred embodiment the fluid containing the desired particles is placed within an inner tube having near the bottom thereof an orifice with a diameter at least equal to that of the diameter of the particles, an air vent and, wherein the inner tube is positioned within an outer tube having a fluid with a density at least equal to that of the solution containing the particles to be separated within the inner tube but less than that of the particles. The application of centrifugal force to the particles directed toward the bottom of the outer tube causes the particles to move through orifice and through the outer solution contained within the outer tube so that the particles are collected from the inner solution, washed by the outer solution, and subsequently sedimented at the bottom of the outer tube.
    Type: Grant
    Filed: August 5, 1981
    Date of Patent: March 6, 1984
    Assignee: Ortho Diagnostic Systems Inc.
    Inventors: Henry A. Graham, Jr., Johnna B. Hawk, Rosemary K. Chachowski
  • Patent number: 4433059
    Abstract: Reagents and methods are described for an immunoassay test of increased sensitivity and decreased complexity employing an immunoglobulin specific for an antigen naturally or artificially placed upon the surface of an indicator particle coupled through the use of a hetero-bifunctional coupling reagent to a second antibody of differing specificity and specific for the antigen to be detected. In a preferred embodiment, a hetero-bifunctional coupling agent couples via a sulfhydryl group, a univalent immunoglobulin specific for the surface antigens on erythrocytes to a second multivalent immunoglobulin through an amide linkage with the latter immunoglobulin wherein said second immunoglobulin is specific for hepatitis-B surface antigen.
    Type: Grant
    Filed: September 8, 1981
    Date of Patent: February 21, 1984
    Assignee: Ortho Diagnostic Systems Inc.
    Inventors: Chi-Deu Chang, Henry A. Graham, Jr.
  • Patent number: 4358436
    Abstract: Methods and reagents for determination of Rh.sub.o (D), C, c, E, e, and K antigens in human blood. The reagents are low-protein compositions containing reduced and S-alkylated IgG antibody to the selected antigen maintained at a pH of between about 7.5 and 8.3. These reagents meet or exceed FDA standards for potency and specificity of the respective antisera.
    Type: Grant
    Filed: June 9, 1980
    Date of Patent: November 9, 1982
    Assignee: Ortho Diagnostics, Inc.
    Inventors: Henry A. Graham, Jr., David J. Olekna, Johnna B. Hawk, Diane B. Kebles
  • Patent number: 4296090
    Abstract: Methods and reagents for determination of Rh (D) antigen in human blood. The reagent is a low-protein composition containing reduced and S-alkylated anti-D IgG maintained at pH of between about 7.5 and 8.3. This reagent meets or exceeds FDA standards for potency of anti-D for the slide test of rapid tube test.
    Type: Grant
    Filed: October 5, 1979
    Date of Patent: October 20, 1981
    Assignee: Ortho Diagnostics, Inc.
    Inventors: Henry A. Graham, Jr., David J. Olekna, Johnna B. Hawk, Diane M. Barrett
  • Patent number: D278653
    Type: Grant
    Filed: August 17, 1982
    Date of Patent: April 30, 1985
    Assignee: Ortho Diagnostic Systems Inc.
    Inventors: Steven R. Savitz, Johnna B. Hawk, Henry A. Graham, Jr., Rosemary K. Chachowski
  • Patent number: D310265
    Type: Grant
    Filed: September 21, 1987
    Date of Patent: August 28, 1990
    Assignee: Ortho Diagnostic Systems Inc.
    Inventors: Dennis M. Mochnal, Henry A. Graham, Jr., Walter J. Yarrow, Jr., C. Minot Dole, Joseph R. Paradis