Abstract: A method for creating large DNA vectors using DNA fragments having different but complementary restriction sites is described. The DNA vector into which the fragments are ligated may contain a clone site which is the same as one of the sites on the fragments. The invention overcomes the limitations of the prior art by allowing the creation of large DNA vector while maintaining unique cloning sites without proliferation of restriction sites, whereas prior art techniques typically increased restriction sites in a vector.

Abstract: A 7,916 base pair nucleic acid fragment from Bacillus megaterium is disclosed. The fragment encodes five proteins, PhaP, PhaQ, PhaR, PhaB, 09 and PhaC, shown or inferred to be involved in the biosynthesis of polyhydroxyalkanoate materials.

Abstract: The present invention relates to genes associated with the tocopherol biosynthesis pathway. More particulary the present invention provides and includes nucleic acid molecules and proteins associated with genes that encode polypeptides associated with the tocopherol biosynthesis pathway. The present invention also provides methods for utilizing such agents, for example in gene isolation, gene analysis, and the production of transgenic plants. Moreover, the present invention includes transgenic plants modified to express the aforementioned polypeptides. In addition, the present invention includes methods for the production of products from the tocopherol biosynthesis pathway as well as in production of plants that are drought resistant.

Abstract: The present invention is in the field of plant genetics and biochemistry. More specifically, the present invention relates to genes and polypeptides associated with the tocopherol biosynthesis pathway, namely those encoding homogentisate prenyl transferase activity, and uses thereof.

Abstract: The present invention is in the field of plant genetics and biochemistry. More specifically, the invention relates to genes associated with the tocopherol biosynthesis pathway. The present invention provides and includes nucleic acid molecules, proteins, and antibodies associated with the genes of the tocopherol biosynthesis pathway. The present invention also provides methods for utilizing such agents, for example in gene isolation, gene analysis and the production of transgenic plants. Moreover, the present invention includes transgenic plants modified to express proteins associated with the tocopherol pathway. In addition, the present invention includes methods for the production of products from the tocopherol biosynthesis pathway.

Abstract: The present invention relates to genes associated with the tocopherol biosynthesis pathway. More particularly, the present invention provides and includes nucleic acid molecules, proteins, and antibodies associated with genes that encode polypeptides that have methyltransferase activity. The present invention also provides methods for utilizing such agents, for example in gene isolation, gene analysis and the production of transgenic plants. Moreover, the present invention includes transgenic plants modified to express the aforementioned polypeptides. In addition, the present invention includes methods for the production of products from the tocopherol biosynthesis pathway.

Abstract: The present invention relates to genes associated with the tocopherol biosynthesis pathway. More particularly, the present invention provides and includes nucleic acid molecules, proteins, and antibodies associated with genes that encode polypeptides that have methyltransferase activity. The present invention also provides methods for utilizing such agents, for example in gene isolation, gene analysis and the production of transgenic plants. Moreover, the present invention includes transgenic plants modified to express the aforementioned polypeptides. In addition, the present invention includes methods for the production of products from the tocopherol biosynthesis pathway.

Abstract: The present invention is in the field of plant genetics and biochemistry. More specifically, the invention relates to genes associated with nucleotide triphosphate transport. The present invention provides and includes nucleic acid molecules, proteins, and antibodies associated with the genes involved in nucleotide triphosphate transport. The present invention also provides methods for utilizing such agents, for example, in gene isolation, gene analysis, and the production of transgenic plants. Moreover, the present invention includes transgenic plants modified to express proteins associated with nucleotide triphosphate transport.

Abstract: The present invention provides and includes nucleic acids, proteins and antibodies associated with novel genes in the MEP pathway. The invention further encompasses methods utilizing such molecules, for example in gene isolation, gene analysis and the production of transgenic plants. The present invention also includes transgenic plants modified to express proteins associated with the MEP pathway.

Abstract: The PHA biosynthetic pathway is combined with a succinic semialdehyde metabolic pathway that metabolizes succinic semialdehyde via a 4-hydroxybutyryl-CoA intermediate in order to produce high levels of PHA comprising 4HB monomer units. This combination is particularly useful, in part, because the PHA biosynthetic pathway is well known and has been expressed to produce P(3HB) to levels as high as 70-80% of the cell dry weight, while the succinic semialdehyde metabolic pathway is also well known, and the combination of the two pathways means that production of PHAs comprising 4HB monomer units does not depend on immediate precursors of 4-hydroxybutyrate. Rather, the production of the PHAs comprising 4HB monomer units can occur simply from the extraction of succinate or succinyl-CoA from the citric acid cycle when the host cell is grown on an inexpensive carbon source such as glucose.