Abstract: The invention relates to a method of culturing mammalian cells expressing a heterologous protein in a perfusion cell culture comprising increasing the potassium concentration and decreasing the molar ratio of sodium to potassium to reduce wasteful cell bleed and to increase protein production. The invention further relates to a serum-free perfusion medium comprising a high potassium ion concentration and a low molar ratio of sodium to potassium and to the use of this medium for use in culturing cells in a perfusion culture during production phase or for reducing the cell bleed volume during production phase.

Abstract: Described herein are systems and methods for presenting building information. In overview, the technologies described herein provide relationships between Building Information Modeling (BIM) data (which includes building schematics defined in terms of standardized three dimensional models) and Building Management System (BMS) data (which includes data indicative of the operation of building components such as HVAC components and the like). Some embodiments use relationships between these forms of data thereby to assist technicians in identifying the physical location of particular pieces of equipment, for example in the context of performing inspections and/or maintenance. In some cases this includes the provision of 2D and/or 3D maps to portable devices, these maps including the location of equipment defined both in BIM and BMS data. In some cases, augmented reality technology is applied thereby to provide richer access to positional information.

Abstract: Hybrid architectures and method methods of operating a display panel are described. In an embodiment, row driver and pixel driver functions are combined in a group of backbone hybrid pixel driver chips, wherein global signal lines are distributed to the backbone hybrid pixel driver chips, where the global signals are manipulated and distributed to a row of pixel driver chips.

Abstract: The present invention provides compounds of Formula I?, or pharmaceutically acceptable salts thereof, pharmaceutical compositions thereof, and methods of use thereof for treating cellular proliferative disorders (e.g., cancer).

Abstract: The present disclosure provides a method of culturing mammalian cells, e.g., by perfusion cell culture, expressing a heterologous protein in a cell culture, comprising culturing mammalian cells expressing a heterologous protein in a culture medium comprising an effective amount of one or more lipids or lipid metabolites selected from the group consisting of: linoleic acid, arachidonic acid, and prostaglandin E2, or derivatives and/or precursors thereof. The lipids or lipid metabolites or combinations thereof can lead to growth suppression and/or increased productivity with reduced cell bleed. The present disclosure also provides methods for increasing the productivity of a cell culture by culturing the cells in a culture medium comprising an effective amount of one or more lipids or lipid metabolites selected from the group consisting of: linoleic acid, arachidonic acid, and prostaglandin E2, or derivatives and/or precursors thereof.

Abstract: Systems, methods and articles of manufacture for determining impact correlations from a calculation graph for calculating taxes. The system includes a computing device, a data store in communication with the computing device and a tax preparation software application executable by the computing device. The tax preparation software application has a tax calculation engine, a tax calculation graph, and an impact chain engine. The tax calculation engine is configured to perform a plurality of tax calculation operations based on the tax calculation graph. The impact chain engine is configured to analyze the tax calculation graph and determine an impact correlation between a change in a value of a first node caused by a change in a value of a second node utilizing an impact chain which includes both the first node and the second node.

Abstract: A dispenser (1) for liquids includes a reservoir (100) defining a volume (V100) for housing a liquid (150); a delivery tube (200) with a first end (201) attached to the reservoir, and a second end (202) distal from the reservoir, the delivery tube including a passage (203) in fluid communication with the volume of the reservoir and extending away from the reservoir; an applicator element (300) including an applicator tip (320) and a base (310) attached to the second end of the delivery tube, the applicator tip having a plurality of fingers (322) extending away from the base to a distal end of the applicator element distal from the delivery tube; and a removable seal (340), wherein removal of the removable seal creates an opening in the passage at a location upstream of the applicator tip and a fluid distribution channel between the fingers.

Abstract: An electronic device includes a display having an active array and a reference array. The active array includes a pixel and the reference array includes a reference pixel. The electronic device also includes processing circuitry communicatively coupled to the display. The processing circuitry is configured to instruct the active array to drive the pixel based at least in part on a set of degradation ratios and gamma tap points for each brightness setting of the display based at least in part on a reconstructed current-voltage curve.

Abstract: The invention relates to proximity-based sortase-mediated protein purification and ligation. Specifically, the invention relates to techniques that links protein expression/purification with conjugation to therapeutic agents, imaging agents, or linkers.

Abstract: An electronic display row drivers or column drivers that send reference currents or voltages to microdrivers to be used to drive micropixels to particular levels. The microdrivers, in turn, ship current to micropixels that display images based at least in part on the shipped current.

Abstract: The present invention is directed to compounds of Formula I, pharmaceutical compositions comprising the same, and their use in the inhibition of HIV protease, the inhibition of HIV replication, the prophylaxis of infection by HIV, the treatment of infection by HIV, and the prophylaxis, treatment, and delay in the onset or progression of AIDS.

Abstract: A mobile electronic device includes a display having an active array and a reference array. The active array includes a pixel and the reference array includes a reference pixel. The mobile electronic device also includes processing circuitry communicatively coupled to the display. The processing circuitry is configured to instruct the active array to drive the pixel based at least in part on a current-voltage relationship of the pixel and a reference current-voltage relationship of the reference pixel.

Abstract: A needle assembly for use with an autonomous ultrasound imaging system includes a needle having a proximal end and a distal end adapted to be inserted into a patient. The needle assembly also includes a needle transducer mounted to an exterior surface of the needle and is electrically coupled to a power source. The needle transducer is configured to receive data signals from the autonomous ultrasound imaging system which contain information relating to a plurality of ultrasound waves generated by an ultrasound probe of the autonomous ultrasound imaging system.

Abstract: Example data management systems and methods are described. In one implementation, a data management system includes a carbon key configured to store at least one authentication factor associated with a user. The system also includes a hardware wallet configured to communicate with the carbon key. The hardware wallet includes a fingerprint sensor configured to scan a fingerprint of the user, a display configured to receive a personal identification number (PIN) from the user; and a system on chip (SOC) configured to authenticate the carbon key, the fingerprint, and the PIN. Additionally, the SOC can sign a transaction based on authentication of at least two of the carbon key, the fingerprint, and the PIN.

Abstract: A method of making depth filtration media, such as for use in a depth filter, are described. The resulting depth filtration media includes a core tube having two or more different layers. The layers can be fibers, such as polymeric or inorganic fibers, wrapped layers of a filter material, or pleated and folded layers of a filter material.

Abstract: A switching power converter is provided with a phase-shifting RC network for phase-shifting a divided version of a drain voltage of a power switch transistor into a phase-shifted voltage. A comparator compares the phase-shifted voltage to a DC bias voltage to detect peaks and valleys during resonant oscillations of the drain voltage of the power switch transistor.

Abstract: The present invention provides solid forms of compounds useful as inhibitors of Acetyl CoA Carboxylase (ACC), compositions thereof, methods of producing the same, and methods of using the same in the treatment of ACC-mediated diseases.

Abstract: An electronic device includes a display having a reference array that includes a first pixel. The display also includes a first emission power supply coupled to the first pixel. The display further includes an active array having a second pixel. The display also includes a second emission power supply coupled to the second pixel.

Abstract: Presented here is a system and method for creating easy-to-understand notifications of the vehicle state. The notification can be visual or can be auditory and can indicate to the user the vehicle state even when the user's attention is directed elsewhere. The indication can serve to attract the user's attention before more involved information is presented to the user, such as text, or the indication can serve to communicate to the user the vehicle state in an easy-to-understand way such as using color-coded displays or audio indications. The intensity of the indication can be adjusted based on the likelihood that indication will be perceived by the user by, for example, measuring ambient light and/or ambient noise. Reducing the intensity of the indication conserves energy of an energy source associated with the vehicle.

Abstract: The present invention relates to stable novel salt forms of N-(cyanomethyl)-4-(2-(4-morpholinophenylamino)pyrimidin-4-yl)benzamide that are suitable for the preparation of pharmaceutical formulations thereof, and their therapeutic use.