Abstract: Disclosed are an optical instrument, an imaging system, and an imaging method for a miniature multi-photon microscope, relating to the optical configuration field. The optical instrument is configured to have a fluorescence excitation optical path and a fluorescence collection optical path. The optical instrument includes a condenser, and the condenser includes a first lens group and a second lens group separately disposed. The first lens group is located in the fluorescence collection optical path, the second lens group is located in the fluorescence excitation optical path and the fluorescence collection optical path. A relative distance the second lens group and a miniature objective is less than a first preset distance threshold. A collection efficiency of scattered fluorescence of the miniature multi-photon microscope may be improved, thereby improving an imaging signal-to-noise ratio and an imaging depth when a sample to be test with a scattering characteristic is observed.

Abstract: The present invention provides a femtosecond pulse laser modulator and a miniature two-photon microscopic imaging device. The femtosecond pulse laser modulator includes a negative dispersion light path and a positive dispersion light path, wherein the negative dispersion light path includes a laser input fiber, which is configured to transmit laser compensated for through prechirp; the positive dispersion light path is located between a femtosecond pulse laser and the negative dispersion light path, and is configured to compensate for, through prechirp, negative dispersion of the laser produced in the laser input fiber. The femtosecond laser modulator provided in the present invention provides distortion-free transmission for the femtosecond pulse laser with a center wavelength in 920 nm, and effectively excites commonly used biological indicators.

Abstract: Protein probes and methods for measuring real-time changes in intracellular superoxide formation are provided. The probes include superoxide sensitive variants of yellow fluorescent and green fluorescent proteins. The probes, or nucleic acids encoding the probes, may be delivered to cells or organisms. Changes in the fluorescence of the probes may then be detected using standard real-time fluoroscopy techniques.

Abstract: Disclosed herein is a bearing assembly comprising: an outer race having an inner surface; an inner race having an outer surface, the inner surface of the outer race and the outer surface of the inner race defining a bearing assembly raceway; a plurality of main rollers positioned within the bearing assembly raceway, the main rollers in contact with the inner surface of the outer race and the outer surface of the inner race; a plurality of mediate rollers positioned within the bearing assembly raceway counter-rotating with the main rollers such that each of the mediate rollers is in rolling contact with a pair of the plurality of main rollers; and a cage positioned in the bearing assembly raceway for at least partially bounding each of the mediate and main rollers.