Abstract: Disclosed are a laser adapter, a multiphoton microscope main unit, an optical system. The laser adapter includes: a shell, and a beam transformation device and a beam stabilization device, which are arranged in the shell. The shell is provided with a laser input port and a laser output port. The beam transformation device is configured to transform a laser beam that enters the shell. The beam stabilization device is disposed downstream of the beam transformation device in a laser transmission direction, and is configured to adjust the laser transmission direction, so as to correct a deviation between an actual position and an ideal position of the laser beam at the laser output port. When lasers with different parameters are input or a laser deflects during transmission, there is no need to readjust an optical path or replace an optical device on the optical path.

Abstract: Disclosed are an imaging device based on multi-photon depth imaging and an imaging probe thereof, and a miniaturized imaging probe is implemented by using a separated structure. During imaging, the imaging probe may be fixedly connected to an imaging object, and an optical signal inside the imaging object may be collected by exciting a multi-photon effect inside the imaging object by excitation light to detect an internal status in the imaging object.

Abstract: Disclosed are an imaging device based on multi-photon depth imaging and an imaging probe thereof, and a miniaturized imaging probe is implemented by using a separated structure. During imaging, the imaging probe may be fixedly connected to the imaging object, and an optical signal inside the imaging object may be collected by exciting a multi-photon effect inside the imaging object through excitation light to detect an internal condition in the imaging object.

Abstract: Disclosed are an optical instrument, an imaging system, and an imaging method for a miniature multi-photon microscope, relating to the optical configuration field. The optical instrument is configured to have a fluorescence excitation optical path and a fluorescence collection optical path. The optical instrument includes a condenser, and the condenser includes a first lens group and a second lens group separately disposed. The first lens group is located in the fluorescence collection optical path, the second lens group is located in the fluorescence excitation optical path and the fluorescence collection optical path. A relative distance the second lens group and a miniature objective is less than a first preset distance threshold. A collection efficiency of scattered fluorescence of the miniature multi-photon microscope may be improved, thereby improving an imaging signal-to-noise ratio and an imaging depth when a sample to be test with a scattering characteristic is observed.

Abstract: The present invention provides a femtosecond pulse laser modulator and a miniature two-photon microscopic imaging device. The femtosecond pulse laser modulator includes a negative dispersion light path and a positive dispersion light path, wherein the negative dispersion light path includes a laser input fiber, which is configured to transmit laser compensated for through prechirp; the positive dispersion light path is located between a femtosecond pulse laser and the negative dispersion light path, and is configured to compensate for, through prechirp, negative dispersion of the laser produced in the laser input fiber. The femtosecond laser modulator provided in the present invention provides distortion-free transmission for the femtosecond pulse laser with a center wavelength in 920 nm, and effectively excites commonly used biological indicators.

Abstract: Protein probes and methods for measuring real-time changes in intracellular superoxide formation are provided. The probes include superoxide sensitive variants of yellow fluorescent and green fluorescent proteins. The probes, or nucleic acids encoding the probes, may be delivered to cells or organisms. Changes in the fluorescence of the probes may then be detected using standard real-time fluoroscopy techniques.