Patents by Inventor Herbert L. Heyneker
Herbert L. Heyneker has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7923221Abstract: The invention relates to processes for producing an immunoglobulin or an immunologically functional immunoglobulin fragment containing at least the variable domains of the immunoglobulin heavy and light chains. The processes can use one or more vectors which produce both the heavy and light chains or fragments thereof in a single cell. The invention also relates to the vectors used to produce the immunoglobulin or fragment, and to cells transformed with the vectors.Type: GrantFiled: April 13, 1995Date of Patent: April 12, 2011Assignees: Genentech, Inc, City of HopeInventors: Shmuel Cabilly, Herbert L. Heyneker, William E. Holmes, Arthur D. Riggs, Ronald B. Wetzel
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Publication number: 20030186226Abstract: The present invention relates to a cost-effective method of assembling long DNA sequences from short synthetic oligonucleotides. More specifically, short oligonucleotides are synthesized in situ on a solid support and subsequently cleaved from the solid support prior to or during the assembly into the full-length DNA sequences.Type: ApplicationFiled: March 7, 2000Publication date: October 2, 2003Inventors: Thomas M. Brennan, Herbert L. Heyneker
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Publication number: 20030068643Abstract: The present invention relates to a method for synthesizing and assembling long DNA sequences from short synthetic oligonucleotides. More specifically, the present invention is a cost-effective method for producing large segments of DNA of interest.Type: ApplicationFiled: November 14, 2002Publication date: April 10, 2003Inventors: Thomas M. Brennan, Herbert L. Heyneker
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Patent number: 6333174Abstract: Methods for the controllable expression in high yields of heterologous proteins under the control of a modified tryptophan promoter-operator system are disclosed. The system employed involves sequential resort to tryptophan repression and derepression, as well as deletion of an attenuator function native to the tryptophan operon. Means are also disclosed for effecting deletions at any given point within a gene fragment and for the ready identification of transformant bacteria via triple ligation procedures.Type: GrantFiled: June 6, 1995Date of Patent: December 25, 2001Assignee: Genentech, Inc.Inventors: Dennis G. Kleid, Daniel G. Yansura, Herbert L. Heyneker, Giuseppe F. Miozzari
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Patent number: 6331415Abstract: The invention relates to processes for producing an immunoglobulin or an immunologically functional immunoglobulin fragment containing at least the variable domains of the immunoglobulin heavy and light chains. The processes can use one or more vectors which produce both the heavy and light chains or fragments thereof in a single cell. The invention also relates to the vectors used to produce the immunoglobulin or fragment, and to cells transformed with the vectors.Type: GrantFiled: June 10, 1988Date of Patent: December 18, 2001Assignee: Genentech, Inc.Inventors: Shmuel Cabilly, Herbert L. Heyneker, William E. Holmes, Arthur D. Riggs, Ronald B. Wetzel
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Patent number: 6264891Abstract: This invention provides an apparatus for preparing chemical libraries. The apparatus includes (1) a carousel comprising a plurality of reaction mounts having at least one reaction well; (2) a rotator that rotates the carousel step-wise; (3) a fluid delivery system; (4) a drain system; and (5) a programmable computer that controls the operation of the apparatus, including the rotator, the fluid delivery system, the drain system and other systems in the apparatus. The preparation of chemical libraries involves rotating the carousel through a plurality of stations. At each station, a physical step in a reaction protocol is carried out on the reaction wells of the mount docked at the station.Type: GrantFiled: May 18, 1999Date of Patent: July 24, 2001Assignee: EOS Biotechnology, Inc.Inventors: Herbert L. Heyneker, Kim D. Ha, David K. Steinmiller, Victor Simonyi
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Patent number: 6140552Abstract: Transgenes for producing recombinant polypeptides transgenic bovine species. A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one expression regulation sequence, a secretory DNA sequence encoding a secretory signal sequence which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptide. Also included are methods for producing transgenic bovine species. The method includes introducing the above transgene into an embryonal target cell of a bovine species, transplanting the transgenic embryonic target cell formed thereby into a recipient bovine parent and identifying at least one female offspring which is capable of producing the recombinant polypeptide in its milk.Type: GrantFiled: June 7, 1995Date of Patent: October 31, 2000Assignee: Pharming B.V.Inventors: Herman A. Deboer, Rein Strijker, Herbert L. Heyneker, Gerard Platenburg, Sang He Lee, Frank Pieper, Paul J. A. Krimpenfort
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Patent number: 6066725Abstract: Transgenes for producing recombinant polypeptides transgenic bovine species are described. A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one expression regulation sequence, a secretory DNA sequence encoding a secretory signal sequence which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptide. Also included are methods for producing transgenic bovine species. The method includes introducing the above transgene into an embryonal target cell of a bovine species, transplanting the transgenic embryonic target cell formed thereby into a recipient bovine parent and identifying at least one female offspring which is capable of producing the recombinant polypeptide in its milk.Type: GrantFiled: September 21, 1998Date of Patent: May 23, 2000Assignee: Pharming B.V.Inventors: Herman A. DeBoer, Rein Strijker, Herbert L. Heyneker, Gerard Platenburg, Sang He Lee, Frank Pieper, Paul J. A. Krimpenfort
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Patent number: 6057100Abstract: The invention relates to novel oligonucleotide arrays.Type: GrantFiled: June 6, 1997Date of Patent: May 2, 2000Assignee: EOS Biotechnology, Inc.Inventor: Herbert L. Heyneker
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Patent number: 6013857Abstract: Transgenes for producing recombinant polypeptides transgenic bovine species. A transgene for producing recombinant polypeptides in the milk of transgenic bovine species comprises at least one expression regulation sequence, a secretory DNA sequence encoding a secretory signal sequence which is functional in mammary secretory cells of the bovine species and a recombinant DNA sequence encoding the recombinant polypeptide. Also included are methods for producing transgenic bovine species. The method includes introducing the above transgene into an embryonal target cell of a bovine species, transplanting the transgenic embryonic target cell formed thereby into a recipient bovine parent and identifying at least one female offspring which is capable of producing the recombinant polypeptide in its milk.Type: GrantFiled: June 5, 1995Date of Patent: January 11, 2000Assignee: Pharming B.V.Inventors: Herman A. Deboer, Rein Strijker, Herbert L. Heyneker, Gerard Platenburg, Sang He Lee, Frank Pieper, Paul J. A. Krimpenfort
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Patent number: 5888808Abstract: The present invention provides recombinant DNA vehicles which are suitable for the microbial expression of DNA encoding a heterologous polypeptide which comprises a portion of the trp operon having the promoter-operator and leader ribosome binding site, and a restriction site providing an insertion site for the DNA sequences encoding the heterologous polypeptide, wherein the restriction site is located 3' of the leader ribosome binding site as a substitute for the Taq I site of the trp promoter-operator and is selected from the group consisting of Xba I and Eco RI. Also provided are E. coli strains transformed with the above described recombinant DNA vehicles.Type: GrantFiled: April 29, 1993Date of Patent: March 30, 1999Assignee: Genentech, Inc.Inventors: Dennis G. Kleid, Daniel G. Yansura, Herbert L. Heyneker, Giuseppe F. Miozzari
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Patent number: 5795745Abstract: Described are methods and means for the construction and microbial expression of quasi-synthetic genes arising from the combination of organic synthesis and enzymatic reverse transcription from messenger RNA sequences incomplete from the standpoint of the desired protein product. Preferred products of expression lack bio-inactivating leader sequences common in eukaryotic expression products but problematic with regard to microbial cleavage to yield bioactive material. Illustrative is a preferred embodiment in which a gene coding for human growth hormone (useful in, e.g., treatment of hypopituitary dwarfism) is constructed and expressed.Type: GrantFiled: June 1, 1995Date of Patent: August 18, 1998Assignee: Genentech, Inc.Inventors: David V. Goeddel, Herbert L. Heyneker
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Patent number: 5756093Abstract: Biologically active variant tissue plasminogen activators are disclosed. The disclosure focuses on position 276 variants that exhibit enhanced fibrin specificity.Type: GrantFiled: July 14, 1994Date of Patent: May 26, 1998Assignee: Genentech, Inc.Inventors: Herbert L. Heyneker, Gordon A. Vehar, Nicholas F. Paoni, William F. Bennett
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Patent number: 5741957Abstract: A transgenic bovine is disclosed whose somatic and germ cells contain a transgene, wherein the transgene comprising a mammary gland specific promoter, a mammary gland specific enhancer, a DNA sequence encoding a signal sequence functional in bovine mammary gland secretory cells and a DNA sequence encoding a heterologous polypeptide of interest wherein the transgenic bovine expresses the transgene such that the polypeptide of interest is detectable in milk produced by the transgenic bovine.Type: GrantFiled: June 5, 1995Date of Patent: April 21, 1998Assignee: Pharming B.V.Inventors: Herman A. Deboer, Rein Strijker, Herbert L. Heyneker, Gerard Platenburg, Sang He Lee, Frank Pieper, Paul J. A. Krimpenfort
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Patent number: 5736134Abstract: Biologically active variant tissue plasminogen activators are disclosed. The disclosure focuses on position 276 variants that exhibit enhanced fibrin specificity.Type: GrantFiled: August 17, 1994Date of Patent: April 7, 1998Assignee: Genentech, In.cInventors: Herbert L. Heyneker, Gordon A. Vehar, Nicholas F. Paoni, William F. Bennett
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Patent number: 5714372Abstract: Biologically active mutant tissue plasminogen activators are disclosed wherein site directed mutagenesis, for example, of a two-chain activation site renders said mutants resistant to conversion to the two-chain form. In addition, mutant tissue plasminogen activators are disclosed which have amino acid substitutions or deletions in the region of positions 274-277, which may or may not be resistant to conversion to the two-chain form, but show enhanced fibrin specificity relative to wild-type tissue plasminogen activator.Type: GrantFiled: September 15, 1994Date of Patent: February 3, 1998Assignee: Genentech, Inc.Inventors: Gordon A. Vehar, Herbert L. Heyneker
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Patent number: 5633076Abstract: A method is disclosed for the production of a transgenic bovine or a transgenic bovine embryo comprising obtaining an ovum from bovine ovaries, maturing the ovum in vitro, fertilizing the mature ovum or ova in vitro to form a zygote, introducing a transgene into the zygote in vitro and maturing the zygote to a preimplantation stage embryo in vitro. To produce the transgenic bovine, the embryo is transplanted into a recipient female bovine, wherein the female bovine gestates the embryo to produce a transgenic bovine.Type: GrantFiled: November 16, 1993Date of Patent: May 27, 1997Assignee: Pharming BVInventors: Herman A. DeBoer, Rein Strijker, Herbert L. Heyneker, Gerard Platenburg, Sang H. Lee, Frank Pieper, Paul J. A. Krimpenfort
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Patent number: 5489529Abstract: High-level expression of bovine growth hormone is achieved using DNA encoding bovine growth hormone which, when transcribed, produces messenger RNA (mRNA) that at its 5' end is substantially free of secondary structure that is capable of interfering with translation of the mRNA.Type: GrantFiled: July 19, 1993Date of Patent: February 6, 1996Inventors: Herman A. de Boer, Herbert L. Heyneker, Peter H. Seeburg
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Patent number: 5424199Abstract: Described are methods and means for the construction and microbial expression of quasi-synthetic genes arising from the combination of organic synthesis and enzymatic reverse transcription from messenger RNA sequences incomplete from the standpoint of the desired protein product. Preferred products of expression lack bio-inactivating leader sequences common in eukaryotic expression products but problematic with regard to microbial cleavage to yield bioactive material. Illustrative is a preferred embodiment in which a gene coding for human growth hormone (useful in, e.g., treatment of hypopituitary dwarfism) is constructed and expressed.Type: GrantFiled: May 27, 1994Date of Patent: June 13, 1995Assignee: Genentech, Inc.Inventors: David V. Goeddel, Herbert L. Heyneker
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Patent number: 5403708Abstract: Methods and compositions for forming ligation product hybridized to a nucleic acid template. The ligation product is formed by contacting a nucleic acid template with a primer capable of hybridizing to the template to form a primed template. The primed template is then contacted with a pool of random extension oligonucleotides of length N and an enzyme system. The enzyme system is capable of covalently linking the primer to an extension oligonucleotide adjacently hybridized to it and one or more other extension oligonucleotides adjacently hybridized to the ligation product defined by covalently linked ligation primer and one or more extension oligonucleotides. When covalently linked, the ligation product is hybridized to the nucleic acid template. Modifications permit the determination of the nucleotide sequence of one or more members of a first set of target nucleotide residues in the nucleic acid template that are spaced at intervals of N nucleotides.Type: GrantFiled: July 6, 1992Date of Patent: April 4, 1995Inventors: Thomas M. Brennan, Herbert L. Heyneker