Abstract: Method for sequencing nucleic acids in which the values of the CID spectrum calculated for an assumed sequence are compared with the measured spectrum of the nucleic acid and the degree of correspondence is calculated, at least one position of the assumed sequence is modified and the degree of correspondence with the measured spectrum is recalculated.

Abstract: Methods for determining nucleic acid length and sequence variation are provided, for example, between an unknown sample and a reference sample. In various embodiments, a method amplifies one or more specific regions of an oligonucleotide molecule in a sample comprising oligonucleotide molecules to produce a sample of amplicons, the sample of amplicons comprising two or more different amplicons. In various embodiments, two or more specific regions are amplified. In various embodiments, the methods (i) denature the amplicons in the sample of amplicons to produce a first set of single-stranded amplicons and a second set of single-stranded amplicons, single stranded amplicons of the second set being complementary to the corresponding single stranded amplicons of the first set and (ii) subject at least the first and second set of single stranded amplicons to mass spectrometric analysis to obtain the masses of the amplicons in the first and second set of single-stranded amplicons.

Abstract: Method for sequencing nucleic acids in which the values of the CID spectrum calculated for an assumed sequence are compared with the measured spectrum of the nucleic acid and the degree of correspondence is calculated, at least one position of the assumed sequence is modified and the degree of correspondence with the measured spectrum is recalculated.

Abstract: Methods and devices based on capillary monolithic columns, preferably consisting of an underivatized poly(styrene-divinylbenzene) monolith, for separating a mixture of polynucleotides by ion pair-reverse phase-high performance chromatography (IP-RP-HPLC).