Abstract: A bioreactor comprising, two chambers in fluid communication, a tissue matrix held in place between, and separating, the two chambers, each side of the tissue matrix in fluid communication with one of the two chambers, each chamber comprising at least one port.

Abstract: There is provided scaffolds for tissue repair/augmentation/implant comprising an acellular matrix, a biocompatible polymer and a biomimetic agent. The scaffolds advantageously supports cell growth in the target tissue. There is also provided a method for the preparation of the scaffold and for monitoring the functionality of the scaffold in tissue using dynamic contrast-enhanced magnetic resonance imaging.

Abstract: A process for preparing biological material for implant in mammal's cardiovascular system, respiratory system or soft tissue is disclosed. The process comprises:(1) isolating from a suitable donor a desired tissue sample of the biological material;(2) extracting the tissue sample with an hypotonic buffer solution at a mild alkaline pH, the buffer solution including active amounts of proteolytic inhibitors and antibiotics;(3) extracting the tissue sample with a buffered solution having a high concentration of salt, the solution being at a mild alkaline pH and including a non-ionic detergent with protease inhibitors and antibiotics;(4) subjecting tissue sample to enzymatic digestion in a buffered saline solution, the enzymes consisting of purified protease-free dioxyribonuclease and ribonuclease;(5) extracting the tissue sample with an anionic detergent at a mild alkaline pH; and(6) storing the tissue sample in physiologic buffered solutions.