Abstract: A pipetting device comprises a retaining cone for holding a slip-on pipette tip and a piston chamber, which communicates with a passage of the retaining cone and contains a reciprocable piston, which acts on the pipette tip that has been fitted on the retaining cone. A filter is provided in the assembly and consists of an aerosol-retaining filter that is detachably mounted in a region which includes the lower end of the pipetting device (1), including the retaining cone (8), as well as the upper end portion of the pipette tip (10) below the retaining cone. The filter may be designed in various ways and may have a progressive filter action. The filter may be arranged in the lower end portion of the retaining cone (8) or in the pipette tip (10) or in an interposed member.

Abstract: Process for the in-vitro biosynthesis of hormones, especially of insulin, by in-vitro conservation or in-vitro propagation of cells from organs and tissues of animal or human origin under suitable cell growth and cell conservation conditions and isolation of the substance formed in a known manner from the culture medium or by processing of the cells. The hormone-producing cells are propagated or conserved in one or more cell culture spaces separated by semipermeable flat membranes of at least one culture medium space.