Abstract: The subject invention provides methods of preparing and/or modifying a working area of interest that results in a level surface layer. In preferred embodiments, a three-dimensional (3-D) paver is utilized to deposit a compressible paving material, wherein, prior to being compacted mechanically, the pavement has a thickness that varies in accordance with the topography of the subgrade surface. Advantageously, paving methods comprising 3-D printing technology provided herein offer a more effective, economical, and versatile solution in preparing level road surface layers than existing paving machines.

Abstract: A system and method for recycling used asphalt material through convection heating and gentle tumbling recycles used asphalt material, such as reclaimed asphalt pavement, by uniformly and gradually applying radiant and convectional heating towards the used asphalt material. A radiant heating coil generates a controlled amount of radiant heat, at about 325 degrees, and up to 750 degrees Fahrenheit. At least one blower works to blow the radiant heat to the used asphalt material as convectional heat. An agitating conveyor simultaneously carries the used asphalt material while it is being heated. The agitating conveyor has a radiant heated conveyor floor that comprises depressions that work to agitate the used asphalt material. The uniform heating of the used asphalt material inhibits moisture from surging from the used asphalt material and maintains integrity of the subsequently formed rejuvenated asphalt. A rejuvenating chamber mixes the used asphalt material with a rejuvenating composition.

Abstract: The subject invention provides methods of preparing and/or modifying a working area of interest that results in a level surface layer. In preferred embodiments, a three-dimensional (3-D) paver is utilized to deposit a compressible paving material, wherein, prior to being compacted mechanically, the pavement has a thickness that varies in accordance with the topography of the subgrade surface. Advantageously, paving methods comprising 3-D printing technology provided herein offer a more effective, economical, and versatile solution in preparing level road surface layers than existing paving machines.

Abstract: Methods are provided for generating, building, updating, and searching a custom database of biological sequences. Methods for differentiating between M. tuberculosis and M. bovis and detecting pyrazinamide (PZA) resistance are also provided.