Abstract: Provided herein are transplantation methods and methods of treating an inflammatory condition, ischemia reperfusion injury, infection, or a wound in a patient, comprising administering isolated IL-33 matrix-bound vesicles to the patient, as well as in vitro-expanded Treg cells. Also provided is a kit comprising IL-33 matrix-bound vesicles and in vitro-expanded Treg cells.

Abstract: Methods are disclosed for treating a subject with a disorder, such as, but not limited to, a) fibrosis of an organ or tissue; b) solid organ transplant rejection; or c) a cardiac disease that is not myocardial infarction or myocardial ischemia. These methods include selecting a subject having or at risk of having the disorder, and administering to the subject a therapeutically effective amount of isolated nanovesicles derived from an extracellular matrix, wherein the nanovesicles contain interleukin (IL)-33 and comprise lysyl oxidase, and wherein the nanovesicles a) do not express CD63 or CD81, or b) are CD63lo CD81lo. In additional embodiments, methods are disclosed for increasing myoblast differentiation.