Patents by Inventor Hidde L. Ploegh
Hidde L. Ploegh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20190046944Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: ApplicationFiled: October 25, 2018Publication date: February 14, 2019Inventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
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Patent number: 10137426Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: GrantFiled: September 16, 2016Date of Patent: November 27, 2018Assignee: President and Fellows of Harvard CollegeInventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
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Publication number: 20180280440Abstract: Methods for the in vitro production of enucleated red blood cells and the enucleated red blood cells thus prepared are provided. Such enucleated red blood cells may express a sortaggable surface protein, which allows for surface modification in the presence of a sortase. Also described herein are surface modified enucleated red blood cells, e.g., conjugated with an agent of interest such as a peptide, a detectable label, or a chemotherapeutic agent, and uses thereof in delivering the agent to a subject.Type: ApplicationFiled: March 23, 2018Publication date: October 4, 2018Applicant: Whitehead Institute for Biomedical ResearchInventors: Harvey Lodish, Hidde L. Ploegh, Hsiang-Ying Sherry Lee, Jiahai Shi, Lenka Hoffman, Novalia Pishesha
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Publication number: 20180280551Abstract: Provided herein are methods for producing site specific PEG modifications to single domain antibodies (e.g., VHHs). Methods for producing site-specific ally conjugated bivalent single domain antibodies (e.g., VHHs) are also provided. Methods for labeling (e.g., with a fluorophore or radionuclide) site-specifically PEGylated single domain antibodies and site-specifically conjugated bivalent single domain antibodies are also provided.Type: ApplicationFiled: October 1, 2016Publication date: October 4, 2018Applicants: Whitehead Institute for Biomedical Research, The General Hospital Corporation d/b/a Massachusetts General HospitalInventors: Mohammad Rashidian, Hidde L. Ploegh, Neil Vasdev, Huan Steven Liang
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Patent number: 10081684Abstract: Methods and reagents for the installation of click chemistry handles on target proteins are provided, as well as modified proteins comprising click chemistry handles. Further, chimeric proteins, for example, bi-specific antibodies, that comprise two proteins conjugated via click chemistry, as well as methods for their generation and use are disclosed herein.Type: GrantFiled: June 28, 2012Date of Patent: September 25, 2018Assignee: Whitehead Institute for Biomedical ResearchInventors: Hidde L. Ploegh, Martin D. Witte, Nicholas C. Yoder
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Publication number: 20170001166Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: ApplicationFiled: September 16, 2016Publication date: January 5, 2017Inventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
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Patent number: 9463431Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: GrantFiled: October 30, 2015Date of Patent: October 11, 2016Assignee: President and Fellows of Harvard CollegeInventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
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Publication number: 20160287734Abstract: The present invention, in some aspects, provides methods, reagents, compositions, and kits for the radiolabeling of proteins, for example, of proteins useful for positron emission tomography (PET) or single-photon emission computed tomography (SPECT) (e.g., for diagnostic and therapeutic applications), using sortase-mediated transpeptidation reactions. Some aspects of this invention provide methods for the conjugation of an agent, for example, a radioactive agent or molecule to diagnostic or therapeutic peptides or proteins. Compositions comprising sortagged, radiolabeled proteins as well as reagents for generating radiolalebed proteins are also provided. Kits comprising reagents useful for the generation of radiolabeled proteins are provided, as are precursor proteins that comprise a sortase recognition motif.Type: ApplicationFiled: November 13, 2014Publication date: October 6, 2016Applicant: Whitehead Institute for Biomedical ResearchInventors: Mohammad Rashidian, Hidde L. Ploegh
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Publication number: 20160122707Abstract: Non-genetically engineered mammalian cells modified by sortase-mediated conjugation of an agent thereto are provided. Methods of conjugating agents to nongenetically engineered mammalian cells using sortase are provided. Methods of using the cells, e.g. a method of modulating an immune response of a subject to an entity of interest, a method of neutralizing a substance in the body of a subject, a method of treating a subject in need of treatment for deficiency of a protein, and a method of treating a subject in need of treatment for a disease, are provided.Type: ApplicationFiled: May 9, 2014Publication date: May 5, 2016Applicant: WHITEHEAD INSTITUTE FOR BIOMEDICAL RESEARCHInventors: Lee Kim Swee, Hidde L. Ploegh
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Publication number: 20160082046Abstract: Methods for the in vitro production of enucleated red blood cells and the enucleated red blood cells thus prepared are provided. Such enucleated red blood cells may express a sortaggable surface protein, which allows for surface modification in the presence of a sortase. Also described herein are surface modified enucleated red blood cells, e.g., conjugated with an agent of interest such as a peptide, a detectable label, or a chemotherapeutic agent, and uses thereof in delivering the agent to a subject.Type: ApplicationFiled: May 9, 2014Publication date: March 24, 2016Applicant: Whitehead Institute for Biomedical ResearchInventors: Harvey Lodish, Hidde L. Ploegh, Hsiang-Ying Lee, Jiahai Shi, Lenka Kundrat, Novalia Pishesha
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Publication number: 20160045885Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: ApplicationFiled: October 30, 2015Publication date: February 18, 2016Inventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
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Patent number: 8940501Abstract: The present invention relates to methods for ligation. The invention provides novel reagents and methods for ligating an acyl donor compound with an acyl acceptor compound. Provided acyl donor compounds comprise a transamidase recognition sequence that allows ligation with a nucleophilic acyl acceptor in the presence of transamidase. The invention further provides kits comprising acyl donor compounds and optionally comprising other reagents for ligation.Type: GrantFiled: February 1, 2010Date of Patent: January 27, 2015Assignee: Whitehead Institute for Biomedical ResearchInventors: Hidde L. Ploegh, John M. Antos, Maximilian Wei-Lin Popp, Carla Guimaraes
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Patent number: 8865479Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: GrantFiled: August 16, 2010Date of Patent: October 21, 2014Assignee: President and Fellows of Harvard CollegeInventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
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Patent number: 8835187Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: GrantFiled: August 6, 2013Date of Patent: September 16, 2014Assignee: Presidents and Fellows of Harvard CollegeInventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
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Patent number: 8835188Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: GrantFiled: August 6, 2013Date of Patent: September 16, 2014Assignee: Presidents and Fellows of Harvard CollegeInventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
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Publication number: 20140249296Abstract: Methods and reagents for the installation of click chemistry handles on target proteins are provided, as well as modified proteins comprising click chemistry handles. Further, chimeric proteins, for example, bi-specific antibodies, that comprise two proteins conjugated via click chemistry, as well as methods for their generation and use are disclosed herein.Type: ApplicationFiled: June 28, 2012Publication date: September 4, 2014Applicant: Whitehead Institute for Biomedical ResearchInventors: Hidde L Ploegh, Martin D. Witte, Nicholas C. Yoder
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Patent number: 8772049Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: GrantFiled: August 6, 2013Date of Patent: July 8, 2014Assignee: President and Fellows of Harvard CollegeInventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
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Publication number: 20140030697Abstract: The present invention, in some aspects, provides methods, reagents, and kits for the functionalization of proteins on the surface of viral particles, for example, of bacteriophages, using sortase-mediated transpeptidation reactions. Some aspects of this invention provide methods for the conjugation of an agent, for example, a detectable label, a binding agent, a click-chemistry handle, or a small molecule to a surface protein of a viral particle. Kits comprising reagents useful for the generation of functionalized viral particles are also provided, as are precursor proteins that comprise a sortase recognition motif, and viral particles comprising such precursor proteins. Nucleic acids encoding viral proteins comprising a sortase recognition motif and expression vectors comprising such nucleic acids are also provided.Type: ApplicationFiled: June 14, 2013Publication date: January 30, 2014Applicants: Massachusetts Institute of Technology, Whitehead Institute for Biomedical ResearchInventors: Hidde L. Ploegh, Gaelen Hess, Carla Guimaraes, Angela Belcher
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Publication number: 20140011709Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: ApplicationFiled: August 6, 2013Publication date: January 9, 2014Applicant: President and Fellows of Harvard CollegeInventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan
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Publication number: 20130338030Abstract: Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody.Type: ApplicationFiled: August 6, 2013Publication date: December 19, 2013Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGEInventors: J. Christopher Love, Hidde L. Ploegh, Jehnna Ronan