Abstract: The present disclosure provides a membrane separation method of a cell suspension which can appropriately separate cells from debris, and a cell culture device. That is, membrane separation processing of the cell suspension is performed using a filtration membrane which includes an inlet-side opening formed on one surface and an outlet-side opening, which is formed on the other surface and communicates with the inlet-side opening, and in which the inlet-side opening and the outlet-side opening are disposed at positions deviated in a direction parallel to the surfaces of the membrane.

Abstract: A culture container has a first inflow port and a first outflow port. The first flow path connects the first outflow port to the first inflow port. A storage container is provided within the first flow path and has a second inflow port which is connected to the first outflow port and a second outflow port which is connected to the first inflow port. A second flow path connects a first region within the first flow path, and a second region within the first flow path. A division processing portion is provided within the second flow path, performs a division process of dividing a cell aggregation flowing in from the first flow path, and allows the cells subjected to the division process to flow out into the first flow path via the second region. A medium supply portion supplies a medium to the inside of the first flow path.

Abstract: There is provided a cell culture bag including: plural tubular portions having gas permeability in which a tube axis direction is directed to a first direction and which are arranged side by side in a second direction intersecting with the first direction and are separated from each other using a partition wall; and communication portions having gas permeability which allow communication between two mutually adjacent tubular portions of the plural tubular portions in an intermediate region R between one end and the other end of the tubular portions.

Abstract: The present disclosure provides a membrane separation method of a cell suspension which can appropriately separate cells from debris, and a cell culture device. That is, membrane separation processing of the cell suspension is performed using a filtration membrane which includes an inlet-side opening formed on one surface and an outlet-side opening, which is formed on the other surface and communicates with the inlet-side opening, and in which the inlet-side opening and the outlet-side opening are disposed at positions deviated in a direction parallel to the surfaces of the membrane.

Abstract: The present disclosure provides a method for subculturing pluripotent stem cells suitable for mass culture. The method for subculturing pluripotent stem cells includes: a culture step of culturing pluripotent stem cells to obtain a cell aggregation; and a dividing step of dividing the cell aggregation by passing the cell aggregation through a mesh-like film, which has a plurality of through-holes each having an opening dimension of 30 ?m to 80 ?m, at a speed of 15 cm/sec to 150 cm/sec.

Abstract: A culture container has a first inflow port and a first outflow port. The first flow path connects the first outflow port to the first inflow port. A storage container is provided within the first flow path and has a second inflow port which is connected to the first outflow port and a second outflow port which is connected to the first inflow port. A second flow path connects a first region within the first flow path, and a second region within the first flow path. A division processing portion is provided within the second flow path, performs a division process of dividing a cell aggregation flowing in from the first flow path, and allows the cells subjected to the division process to flow out into the first flow path via the second region. A medium supply portion supplies a medium to the inside of the first flow path.

Abstract: There is provided a cell culture bag including: plural tubular portions having gas permeability in which a tube axis direction is directed to a first direction and which are arranged side by side in a second direction intersecting with the first direction and are separated from each other using a partition wall; and communication portions having gas permeability which allow communication between two mutually adjacent tubular portions of the plural tubular portions in an intermediate region R between one end and the other end of the tubular portions.

Abstract: The present invention provides a polarizing plate that includes a polarizer, and an optical film including an alignment layer, an optically anisotropic layer, and an optically isotropic acrylic polymer layer on at least one surface of the polarizer, in which the optically anisotropic layer is a layer formed by irradiating a polymerizable composition including a liquid crystal compound that is directly applied to the alignment layer with light to polymerize the liquid crystal compound, the acrylic polymer layer is a layer formed by curing a polymerizable composition including (meth)acrylate that is directly applied to a surface of the layer formed from the polymerizable composition including a liquid crystal compound, and the thickness of the acrylic polymer layer is larger than the thickness of the optically anisotropic layer. According to the present invention, it is possible to provide a polarizing plate having a small thickness.

Abstract: The polarizing plate fabrication method includes the following: (1) preparing a transfer material including a temporary support and a transfer body including an optical anisotropic layer and an optical anisotropic layer; (2) peeling the temporary support and separating it from the transfer body; and (3) adhering the transfer body to a film including a polarizer, in which both the optical anisotropic layer and the optical anisotropic layer are layers formed of a polymerizable composition including a liquid crystal compound applied onto the temporary support, and the optical anisotropic layer and the optical anisotropic layer both have in-plane retardation, and a difference between slow axis directions in the optical anisotropic layer and the optical anisotropic layer is in a range of 3° to 90°. The fabrication method allows adhering of an optical anisotropic layer having a variety of optical compensation capabilities to a variety of polarizers in a minimum constitution.

Abstract: The present invention provides a polarizing plate that includes a polarizer, and an optical film including an alignment layer, an optically anisotropic layer, and an optically isotropic acrylic polymer layer on at least one surface of the polarizer, in which the optically anisotropic layer is a layer formed by irradiating a polymerizable composition including a liquid crystal compound that is directly applied to the alignment layer with light to polymerize the liquid crystal compound, the acrylic polymer layer is a layer formed by curing a polymerizable composition including (meth)acrylate that is directly applied to a surface of the layer formed from the polymerizable composition including a liquid crystal compound, and the thickness of the acrylic polymer layer is larger than the thickness of the optically anisotropic layer. According to the present invention, it is possible to provide a polarizing plate having a small thickness.

Abstract: The present invention provides a polarizing plate that includes a polarizer and an optically anisotropic layer that is a layer formed on at least one surface of the polarizer by irradiating a polymerizable composition including a liquid crystal compound with light to polymerize the liquid crystal compound, in which only an adhesive layer or only an adhesive layer and a protective film provided on a surface of the polarizer are provided between the optically anisotropic layer and the polarizer, and a method for producing the polarizing plate including laminating a transfer material including a temporary support and an optically anisotropic layer on a film including a polarizer and then peeling off the temporary support of the transfer material. According to the present invention, it is possible to provide a polarizing plate having a small thickness.

Abstract: The method includes: forming a coating film including particles and an ultraviolet curable resin on a film substrate; and curing the ultraviolet curable resin by ultraviolet irradiation to form an anti-glare layer constituted by the particles and the ultraviolet curable resin. A ratio of a thickness A of the anti-glare layer to a diameter B of the particles is in the range of 1.5 to 2.4. The ultraviolet irradiation is performed with two or more ultraviolet irradiation units, the first unit irradiation amount is 49 mJ/cm2 or less, the second and subsequent units irradiation amount is greater than 50 mJ/cm2, and the total irradiation amount is 100 mJ/cm2 or greater. The oxygen concentration at a position which is 1 mm above a surface of the coating film is 1 ppm to 2000 ppm. The first unit irradiation is performed above a roller with surface temperature of 10° C. to 24° C.

Abstract: The method includes: forming a coating film including particles and an ultraviolet curable resin on a film substrate; and curing the ultraviolet curable resin by ultraviolet irradiation to form an anti-glare layer constituted by the particles and the ultraviolet curable resin. A ratio of a thickness A of the anti-glare layer to a diameter B of the particles is in the range of 1.5 to 2.4. The ultraviolet irradiation is performed with two or more ultraviolet irradiation units, the first unit irradiation amount is 49 mJ/cm2 or less, the second and subsequent units irradiation amount is greater than 50 mJ/cm2, and the total irradiation amount is 100 mJ/cm2 or greater. The oxygen concentration at a position which is 1 mm above a surface of the coating film is 1 ppm to 2000 ppm. The first unit irradiation is performed above a roller with surface temperature of 10° C. to 24° C.

Abstract: First image data read out from an image information apparatus is stored in a page memory through a buffer memory and is also displayed on a CRT (cathode ray tube) display unit. A second image data subsequently read is converted into a predetermined size under the control of a CPU (central processing unit) and stored in the first image data stored in the page memory in a superimposed fashion.

Abstract: An image data processing apparatus including a scanner for individually reading first and second image information from first and second documents, a page memory for storing the first image information into its first memory area, a display device for displaying a first image corresponding to the first image information stored in the first memory area of the page memory, a keyboard or a mouse for defining a desired range to be substituted with a second image corresponding to the second image information, in the first image displayed on the display device, a CPU for determining a magnifying ratio of the second image suited to the desired range, according to the sizes of the desired range and the second image, an enlargement/reduction circuit for magnifying the second image in response to the magnifying ratio determined by the CPU and the CPU for further processing a storage of the second image information read by the scanner into a second memory area corresponding to the desired range in the first memory area,