Patents by Inventor Hideaki Yamaoka
Hideaki Yamaoka has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Biosensor incorporating protein-immobilized membrane and method of immobilizing protein in biosensor
Patent number: 9702843Abstract: The present invention relates to a protein-immobilized membrane (14) including a cell membrane homologous layer (14A) and a protein (14B) immobilized to the cell membrane homologous layer (14A), where the protein contains cytochrome or a cytochrome complex. The present invention also relates to a method for forming a protein-immobilized membrane (14), and an enzyme-immobilized electrode and a biosensor (X1) provided with a protein-immobilized membrane (14). Preferably, the cell membrane homologous layer (14A) may contain a phospholipid polymer, and the protein (14B) may be CyGDH including an ? subunit having a glucose dehydrogenase activity and cytochrome C having a function of electron transfer.Type: GrantFiled: September 10, 2015Date of Patent: July 11, 2017Assignee: ARKRAY, Inc.Inventors: Koji Katsuki, Hideaki Yamaoka -
BIOSENSOR INCORPORATING PROTEIN-IMMOBILIZED MEMBRANE AND METHOD OF IMMOBILIZING PROTEIN IN BIOSENSOR
Publication number: 20150377818Abstract: The present invention relates to a protein-immobilized membrane (14) including a cell membrane homologous layer (14A) and a protein (14B) immobilized to the cell membrane homologous layer (14A), where the protein contains cytochrome or a cytochrome complex. The present invention also relates to a method for forming a protein-immobilized membrane (14), and an enzyme-immobilized electrode and a biosensor (X1) provided with a protein-immobilized membrane (14). Preferably, the cell membrane homologous layer (14A) may contain a phospholipid polymer, and the protein (14B) may be CyGDH including an ? subunit having a glucose dehydrogenase activity and cytochrome C having a function of electron transfer.Type: ApplicationFiled: September 10, 2015Publication date: December 31, 2015Inventors: Koji Katsuki, Hideaki Yamaoka -
Patent number: 9206398Abstract: The present invention relates to a method for purifying target protein which contains electron transfer protein, from a protein solution containing the target protein, by means of liquid chromatography. The liquid chromatography is performed as follows: First, the protein solution is introduced into a column which is filled with a packing agent, thereby causing the packing agent to bond to the target protein. Then, impurities are removed, and then the target protein is eluted from the packing agent in an eluent which contains a hydroxy-cholate. An example of the target protein is glucose dehydrogenase which contains a protein having glucose dehydrogenation activity. The liquid chromatography is performed as a combination of hydrophobic chromatography and anion exchange chromatography.Type: GrantFiled: December 1, 2011Date of Patent: December 8, 2015Assignee: ARKRAY, Inc.Inventors: Hideaki Yamaoka, Keisuke Kurosaka, Shido Kawase
-
Publication number: 20120309072Abstract: The present invention relates to a method for purifying target protein which contains electron transfer protein, from a protein solution containing the target protein, by means of liquid chromatography. The liquid chromatography is performed as follows: First, the protein solution is introduced into a column which is filled with a packing agent, thereby causing the packing agent to bond to the target protein. Then, impurities are removed, and then the target protein is eluted from the packing agent in an eluent which contains a hydroxy-cholate. An example of the target protein is glucose dehydrogenase which contains a protein having glucose dehydrogenation activity. The liquid chromatography is performed as a combination of hydrophobic chromatography and anion exchange chromatography.Type: ApplicationFiled: December 1, 2011Publication date: December 6, 2012Applicant: ARKRAY, INC.Inventors: Hideaki YAMAOKA, Keisuke KUROSAKA, Shido KAWASE
-
Patent number: 8210349Abstract: A sensor storage container is provided that allows sensors to be checked visually from the outside. A container is used for storing sensors including an oxidation-reduction enzyme that oxidizes or reduces an object to be analyzed, a mediator that mediates the transfer of electrons caused by oxidation or reduction, and a detection means that detects the oxidation or reduction reaction. The container is transparent or semi-transparent in whole or in part and thereby the sensors can be checked visually from the outside of the container. This container may be composed of a container body and a lid. The container body may be provided with a scale. Preferably, the sensors are those produced using a ruthenium metal complex as the mediator.Type: GrantFiled: March 25, 2004Date of Patent: July 3, 2012Assignee: ARKRAY, Inc.Inventor: Hideaki Yamaoka
-
Patent number: 8012729Abstract: Substrate specificity for glucose of a glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 13 is improved by substituting another amino acid residue for the amino acid residue at position 472 and/or 475.Type: GrantFiled: August 23, 2010Date of Patent: September 6, 2011Assignee: Arkray, Inc.Inventor: Hideaki Yamaoka
-
Publication number: 20110076707Abstract: Substrate specificity for glucose of a glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 13 is improved by substituting another amino acid residue for the amino acid residue at position 472 and/or 475.Type: ApplicationFiled: August 23, 2010Publication date: March 31, 2011Applicant: Arkray, Inc.Inventor: Hideaki Yamaoka
-
Patent number: 7824881Abstract: The present invention relates to a technique for measuring a glucose level by utilizing a reaction system containing an enzyme and an electron carrier. In accordance with the glucose level measuring method of the present invention, glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier. The present invention further provides a glucose sensor in which glucose dehydrogenase with cytochrome C attached thereto or glucose dehydrogenase derived from derived from a microorganism belonging to a burkholderia genus is used as the enzyme, and a Ru compound is used as the electron carrier.Type: GrantFiled: January 10, 2008Date of Patent: November 2, 2010Assignee: ARKRAY, Inc.Inventors: Hideaki Yamaoka, Mitsuhiro Hoshijima, Tomomichi Tsujimoto
-
Patent number: 7803592Abstract: Substrate specificity for glucose of a glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 13 is improved by substituting another amino acid residue for the amino acid residue at position 472 and/or 475.Type: GrantFiled: April 22, 2005Date of Patent: September 28, 2010Assignee: Arkray, Inc.Inventor: Hideaki Yamaoka
-
Patent number: 7780828Abstract: The present invention relates to an analytical instrument (1) which includes a flow path (5) for moving a sample containing blood cells, an introduction port (50) for introducing the sample into the flow path (5), a reagent portion (51) arranged in the flow path (5), and an electron detection medium (52) for obtaining information necessary for analyzing an analysis target component contained in the sample in relation with an amount of electrons transferred. The reagent portion (51) contains an electron mediator for supplying an electron taken from the analysis target component in the sample to the electron detection medium (52), and at least part of the reagent portion is positioned adjacent to the introduction port (50).Type: GrantFiled: January 7, 2005Date of Patent: August 24, 2010Assignee: ARKRAY, Inc.Inventors: Hideaki Yamaoka, Kenji Nagakawa, Mitsuhiro Hoshijima, Sadaaki Kimura
-
Patent number: 7713718Abstract: A process for producing glucose dehydrogenases. This process comprises transferring a DNA containing a sequence represented by SEQ ID NO:1 which encodes an ? subunit having a glucose dehydrogenase activity and a ? subunit being an electron transfer protein into a microorganism belonging to the genus Pseudomonas to thereby construct a transformant, and culturing this transformant so as to allow the production of a first glucose dehydrogenase containing the above-described ? subunit and a second glucose dehydrogenase free from the ? subunit. The ? subunit as described above has a molecular weight of about 60 kDa measured by, for example, SDS-polyacrylamide gel electrophoresis under reducing conditions, while the ? subunit as described above has a molecular weight of about 43 kDa measured by, for example, SDS-polyacrylamide gel electrophoresis under reducing conditions.Type: GrantFiled: August 20, 2003Date of Patent: May 11, 2010Assignee: ARKRAY, Inc.Inventors: Koji Sode, Hideaki Yamaoka, Mitsuhiro Hoshijima, Keisuke Kurosaka, Shido Kawase
-
Publication number: 20100086989Abstract: A mutant glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 3 or an amino acid sequence of SEQ ID NO: 3 including substitution, deletion, insertion or addition of one or more amino acid residues other than the amino acid residue at the 365th position and having glucose dehydrogenase activity, wherein an amino acid residue at a position corresponding to the 365th position of, the amino acid sequence is replaced with another amino acid residue, and the mutant glucose dehydrogenase shows an improved substrate specificity to glucose.Type: ApplicationFiled: September 9, 2009Publication date: April 8, 2010Applicant: Arkray, Inc.Inventors: Hideaki Yamaoka, Masashi Tsukada
-
Patent number: 7604969Abstract: A mutant glucose dehydrogenase having an improved substrate specificity to glucose, which is a protein comprising the amino acid sequence depicted in SEQ ID NO:3 or an amino acid sequence having the substitution, deletion, insertion or addition of one or more amino acid residues excluding amino acid 365 in the amino acid sequence depicted in SEQ ID NO:3 and having a glucose dehydrogenase activity, the amino acid residue corresponding to amino acid 365 being substituted by other amino acid residue.Type: GrantFiled: June 12, 2006Date of Patent: October 20, 2009Assignee: Arkray, Inc.Inventors: Hideaki Yamaoka, Masashi Tsukada
-
Publication number: 20090101499Abstract: The present invention relates to a protein-immobilized membrane (14) including a cell membrane homologous layer (14A) and a protein (14B) immobilized to the cell membrane homologous layer (14A), where the protein contains cytochrome or a cytochrome complex. The present invention also relates to a method for forming a protein-immobilized membrane (14), and an enzyme-immobilized electrode and a biosensor (X1) provided with a protein-immobilized membrane (14). Preferably, the cell membrane homologous layer (14A) may contain a phospholipid polymer, and the protein (14B) may be CyGDH including an ? subunit having a glucose dehydrogenase activity and cytochrome C having a function of electron transfer.Type: ApplicationFiled: May 18, 2006Publication date: April 23, 2009Applicant: ARKRAY, INC.Inventors: Koji Katsuki, Hideaki Yamaoka
-
Publication number: 20090017483Abstract: The present invention relates to an analytical instrument (1) which includes a flow path (5) for moving a sample containing blood cells, an introduction port (50) for introducing the sample into the flow path (5), a reagent portion (51) arranged in the flow path (5), and an electron detection medium (52) for obtaining information necessary for analyzing an analysis target component contained in the sample in relation with an amount of electrons transferred. The reagent portion (51) contains an electron mediator for supplying an electron taken from the analysis target component in the sample to the electron detection medium (52), and at least part of the reagent portion is positioned adjacent to the introduction port (50).Type: ApplicationFiled: January 7, 2005Publication date: January 15, 2009Inventors: Hideaki Yamaoka, Kenji Nagakawa, Mitsuhiro Hoshijima, Sadaaki Kimura
-
Publication number: 20080280312Abstract: Substrate specificity for glucose of a glucose dehydrogenase having the amino acid sequence of SEQ ID NO: 13 is improved by substituting another amino acid residue for the amino acid residue at position 472 and/or 475.Type: ApplicationFiled: April 22, 2005Publication date: November 13, 2008Inventor: Hideaki Yamaoka
-
Publication number: 20080206833Abstract: A mutant glucose dehydrogenase having an improved substrate specificity to glucose, which is a protein comprising the amino acid sequence depicted in SEQ ID NO:3 or an amino acid sequence having the substitution, deletion, insertion or addition of one or more amino acid residues excluding amino acid 365 in the amino acid sequence depicted in SEQ ID NO:3 and having a glucose dehydrogenase activity, the amino acid residue corresponding to amino acid 365 being substituted by other amino acid residue.Type: ApplicationFiled: June 12, 2006Publication date: August 28, 2008Applicant: ARKRAY, INC.Inventors: Hideaki Yamaoka, Masashi Tsukada
-
Concentration measuring method, concentration test instrument, and concentration measuring apparatus
Patent number: 7390391Abstract: The present invention relates to technology for constructing a reaction system including a test target, an oxidation-reduction enzyme, and an electron mediator, and measuring the concentration of the test target by an electrochemical process. A Ru compound is used as the electron mediator. The present invention provides a concentration test instrument including a substrate, first and second electrodes formed on the substrate, and a reagent layer formed as a solid. The reagent layer contains an oxidation-reduction enzyme and a Ru compound, and is constituted so as to dissolve and construct a liquid phase reaction system when a sample liquid is supplied.Type: GrantFiled: August 30, 2002Date of Patent: June 24, 2008Assignee: Arkray, Inc.Inventors: Kenji Nagakawa, Hideaki Yamaoka -
Concentration measuring method, concentration test instrument, and concentration measuring apparatus
Patent number: RE44522Abstract: The present invention relates to technology for constructing a reaction system including a test target, an oxidation-reduction enzyme, and an electron mediator, and measuring the concentration of the test target by an electrochemical process. A Ru compound is used as the electron mediator. The present invention provides a concentration test instrument including a substrate, first and second electrodes formed on the substrate, and a reagent layer formed as a solid. The reagent layer contains an oxidation-reduction enzyme and a Ru compound, and is constituted so as to dissolve and construct a liquid phase reaction system when a sample liquid is supplied.Type: GrantFiled: June 24, 2010Date of Patent: October 8, 2013Assignee: Arkray, Inc.Inventors: Kenji Nagakawa, Hideaki Yamaoka -
Concentration measuring method, concentration test instrument, and concentration measuring apparatus
Patent number: RE45764Abstract: The present invention relates to technology for constructing a reaction system including a test target, an oxidation-reduction enzyme, and an electron mediator, and measuring the concentration of the test target by an electrochemical process. A Ru compound is used as the electron mediator. The present invention provides a concentration test instrument including a substrate, first and second electrodes formed on the substrate, and a reagent layer formed as a solid. The reagent layer contains an oxidation-reduction enzyme and a Ru compound, and is constituted so as to dissolve and construct a liquid phase reaction system when a sample liquid is supplied.Type: GrantFiled: August 28, 2013Date of Patent: October 20, 2015Assignee: ARKRAY, Inc.Inventors: Kenji Nagakawa, Hideaki Yamaoka