Patents by Inventor Hidefumi Kuwata

Hidefumi Kuwata has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHOD FOR PRODUCING MICROPARTICLES
    Publication number: 20170348236
    Abstract: The present invention has an object providing microparticles having an average particle size of 100 ?m or less. The present invention provides microparticles having an average particle size of 100 ?m or less and a method for producing thereof. In addition, the present invention provides medicine, food and feedstuff comprising the microparticles having an average particle size of 100 ?m or less.
    Type: Application
    Filed: June 14, 2017
    Publication date: December 7, 2017
    Inventor: Hidefumi KUWATA
  • METHOD FOR PRODUCING MICROPARTICLES
    Publication number: 20160206562
    Abstract: The present invention has an object providing microparticles having an average particle size of 100 ?m or less. The present invention provides microparticles having an average particle size of 100 ?m or less and a method for producing thereof. In addition, the present invention provides medicine, food and feedstuff comprising the microparticles having an average particle size of 100 ?m or less.
    Type: Application
    Filed: August 20, 2014
    Publication date: July 21, 2016
    Inventor: Hidefumi KUWATA
  • Method of disulfide crosslink forming in vitro protein synthesis
    Patent number: 8603775
    Abstract: It is an objective of the present invention to provide a method of synthesizing a protein having disulfide bonds using a reconstituted protein synthesizing system in a convenient manner with high efficiency. It has been found that a protein having the activity can be obtained and synthesized with good efficiency by using a reconstituted protein synthesizing system comprising purified components, from which enzymes and substrates influencing an oxidation-reduction state are removed and in which a state of redox equilibrium between disulfide and thiol is artificially regulated. Such system is used instead of a cell extract or a crude fraction thereof that contains various enzymes and substrates that maintain a reduced state, such as thioredoxin reductase [EC 1.6.4.5] and glutathion reductase [EC 1.6.4.2], resulting in difficulty in conditioning a redox state.
    Type: Grant
    Filed: April 28, 2005
    Date of Patent: December 10, 2013
    Assignee: Biocomber Co., Ltd.
    Inventor: Hidefumi Kuwata
  • Method of disulfide crosslink forming in vitro protein synthesis
    Publication number: 20090162884
    Abstract: It is an objective of the present invention to provide a method of synthesizing a protein having disulfide bonds using a reconstituted protein synthesizing system in a convenient manner with high efficiency. It has been found that a protein having the activity can be obtained and synthesized with good efficiency by using a reconstituted protein synthesizing system comprising purified components, from which enzymes and substrates influencing an oxidation-reduction state are removed and in which a state of redox equilibrium between disulfide and thiol is artificially regulated. Such system is used instead of a cell extract or a crude fraction thereof that contains various enzymes and substrates that maintain a reduced state, such as thioredoxin reductase [EC 1.6.4.5] and glutathion reductase [EC 1.6.4.2], resulting in difficulty in conditioning a redox state.
    Type: Application
    Filed: April 28, 2005
    Publication date: June 25, 2009
    Inventor: Hidefumi Kuwata