Patents by Inventor Hidehiko Sugiyama

Hidehiko Sugiyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of detecting nucleic acid
    Patent number: 5728531
    Abstract: A method of detecting nucleic acid utilizes hybridization between the nucleic acid to be detected which is present on an immobilizing support and a DNA probe which is complementary to at least a portion of the desired nucleotide sequence of the nucleic acid. The present DNA probe includes a hapten attached via a linker which a hapten is gibberellin or a gibberellin derivative. After hybridization by reaction between the nucleic acid to be detected and the DNA probe, the unreacted DNA probe is removed, and then labelled anti-hapten antibody is allowed to bind with the DNA probe of the hybrid and the labelling is used to detect the nucleic acid.Accordingly, stable detecting sensitivity may be achieved regardless of the label mixing ratio or labelling ratio, and thus regardless of the nucleotide sequence composition of the nucleic acid being tested.
    Type: Grant
    Filed: March 29, 1996
    Date of Patent: March 17, 1998
    Assignees: Kabushiki Kaisha Toyota Chuo Kenkyusho, Aisin Seiki Kabushiki Kaisha
    Inventors: Yukio Yamada, Osamu Asami, Hiroko Uchiyama, Hidehiko Sugiyama, Satoshi Fujita, Takekazu Yamamoto, Naoto Kagiyama, Masayoshi Momiyama, Yasumitsu Kondoh
  • Polypeptide possessing protein disulfide isomerase activity gene encoding the same and process for producing the same
    Patent number: 5700659
    Abstract: A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polyeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20.degree. to 70.degree. C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins.
    Type: Grant
    Filed: June 5, 1995
    Date of Patent: December 23, 1997
    Assignee: Kabushiki Kaisha Toyota Chuo Kenkusho
    Inventors: Yukio Yamada, Osamu Asami, Hidehiko Sugiyama, Chie Idekoba, Fumihiko Hoshino, Masana Hirai, Tsutomu Kajino, Takao Imaeda, Kiyoko Sarai
  • Polypeptide from Humicola insolens possessing protein disulfide isomerase activity gene encoding the same
    Patent number: 5496719
    Abstract: A highly thermostable polypeptide possessing protein disulfide isomerase (PDI) activity, a gene coding for the polypeptide and a process for producing the polypeptide are provided. The polypeptide possessing PDI activity is characterized by A) having a capability of catalyzing a disulfide exchange in proteins, B) recognizing mainly ribonuclease A as a substrate, C) having a suitable active temperature of 20.degree. to 70.degree. C., D) being stable at a pH value of 6 to 9, and E) having a molecular weight of about 60,000 to 62,000. Since it has a higher thermostability and exhibits a stable activity in a wider dithiothreitol concentration range as compared with the conventional PDI, it is possible to provide a novel enzyme active protein which can be advantageously used for a refolding reaction of certain proteins.
    Type: Grant
    Filed: May 27, 1993
    Date of Patent: March 5, 1996
    Assignee: Kabushiki Kaisha Toyota Chuo Kenkyusho
    Inventors: Yukio Yamada, Osamu Asami, Hidehiko Sugiyama, Chie Idekoba, Fumihiko Hoshino, Masana Hirai, Tsutomu Kajino, Takao Imaeda, Kiyoko Sarai