Patents by Inventor Hidemi Fujii

Hidemi Fujii has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Coryneform bacterium and method for producing heterologous fusion proteins
    Patent number: 10077447
    Abstract: The present invention provides a coryneform bacterium having an ability to produce a heterologous fusion protein by secretory production, which has been modified to express a genetic construct for secretory production of the heterologous fusion protein encoding at least a heterologous fusion protein comprising an extein and an intein having an activity of acyl rearrangement. The method for producing proteins modified at the C-terminus is also provided.
    Type: Grant
    Filed: October 27, 2015
    Date of Patent: September 18, 2018
    Assignee: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Veronika Aleksandrovna Kotliarova, Hidemi Fujii, Masakazu Sugiyama
  • Method for producing peptide hydrazide, peptide amide, and peptide thioester
    Patent number: 10053491
    Abstract: An object of the present invention is to provide a method for producing a peptide thioester compound, a peptide hydrazide compound, and a peptide amide compound. The present invention provides a method for producing a peptide hydrazide compound or a peptide amide compound by using a compound represented by Formula (2): wherein R1, R2, R3, and X are as defined in the specification, and using a hydrazine compound or an ammonia compound as a reaction reagent. The present invention also provides a method for producing a peptide thioester compound from the peptide hydrazide compound.
    Type: Grant
    Filed: May 4, 2016
    Date of Patent: August 21, 2018
    Assignee: AJINOMOTO CO., INC.
    Inventors: Akira Otaka, Akira Shigenaga, Masakazu Sugiyama, Hidemi Fujii
  • METHOD FOR PRODUCING PEPTIDE HYDRAZIDE, PEPTIDE AMIDE, AND PEPTIDE THIOESTER
    Publication number: 20160264626
    Abstract: An object of the present invention is to provide a method for producing a peptide thioester compound, a peptide hydrazide compound, and a peptide amide compound. The present invention provides a method for producing a peptide hydrazide compound or a peptide amide compound by using a compound represented by Formula (2): wherein R1, R2, R3, and X are as defined in the specification, and using a hydrazine compound or an ammonia compound as a reaction reagent. The present invention also provides a method for producing a peptide thioester compound from the peptide hydrazide compound.
    Type: Application
    Filed: May 4, 2016
    Publication date: September 15, 2016
    Applicant: AJINOMOTO CO., INC.
    Inventors: Akira OTAKA, Akira Shigenaga, Masakazu Sugiyama, Hidemi Fujii
  • Coryneform Bacterium and Method for Producing Heterologous Fusion Proteins
    Publication number: 20160060640
    Abstract: The present invention provides a coryneform bacterium having an ability to produce a heterologous fusion protein by secretory production, which has been modified to express a genetic construct for secretory production of the heterologous fusion protein encoding at least a heterologous fusion protein comprising an extein and an intein having an activity of acyl rearrangement. The method for producing proteins modified at the C-terminus is also provided.
    Type: Application
    Filed: October 27, 2015
    Publication date: March 3, 2016
    Applicant: AJINOMOTO CO., INC.
    Inventors: Sergey Vasilievich Smirnov, Veronika Aleksandrovna Kotliarova, Hidemi Fujii, Masakazu Sugiyama
  • METHOD FOR PRODUCING MONATIN USING AN L-AMINO ACID AMINOTRANSFERASE
    Publication number: 20140234916
    Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.
    Type: Application
    Filed: March 31, 2014
    Publication date: August 21, 2014
    Applicant: Ajinomoto Co., Inc.
    Inventors: Yasuaki Takakura, Hiroomi Ogino, Masakazu Sugiyama, Kenichi Mori, Eri Tabuchi, Koki Ishikawa, Uno Tagami, Hidemi Fujii
  • Method for producing monatin using an L-amino acid aminotransferase
    Patent number: 8771997
    Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.
    Type: Grant
    Filed: October 12, 2011
    Date of Patent: July 8, 2014
    Assignee: Ajinomoto Co., Inc.
    Inventors: Yasuaki Takakura, Hiroomi Ogino, Masakazu Sugiyama, Kenichi Mori, Eri Tabuchi, Koki Ishikawa, Uno Tagami, Hidemi Fujii
  • METHOD FOR PRODUCING MONATIN
    Publication number: 20120270279
    Abstract: The present invention provides a methodology for improving a yield of 2R,4R-Monatin. Specifically, the present invention provides a method for producing 2S,4R-Monatin or a salt thereof, comprising contacting 4R-IHOG with an L-amino acid aminotransferase in the presence of an L-amino acid to form the 2S,4R-Monatin; a method for producing 2R,4R-Monatin or a salt thereof, comprising isomerizing the 2S,4R-Monatin to form the 2R,4R-Monatin; and the like. These production methods may further comprise condensing indole-3-pyruvate and pyruvate to form the 4R-IHOG, and deaminating a tryptophan to form the indole-3-pyruvate.
    Type: Application
    Filed: October 12, 2011
    Publication date: October 25, 2012
    Applicant: AJINOMOTO CO. INC
    Inventors: Yasuaki Takakura, Hiroomi Ogino, Masakazu Sugiyama, Kenichi Mori, Eri Tabuchi, Koki Ishikawa, Uno Tagami, Hidemi Fujii