Abstract: A method of evaluating a function of an organ for transplantation harvested from a living body, the method includes preparing data representing change over time in content of marker substances in accordance with a perfusion, taking a first tissue sample from the organ for transplantation at first timing in a reperfusion of the organ for transplantation, taking a second tissue sample from the organ for transplantation at second timing after the first timing in the reperfusion, measuring contents of the marker substances in the first tissue sample, measuring contents of the marker substances in the second tissue sample, calculating change in content at the second timing as compared with the first timing for each of the marker substances, and calculating an indicator relating to evaluation of the function of the organ for transplantation using the calculated change in content and the data.

Abstract: An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.

Abstract: An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.

Abstract: A solution containing double stranded nucleic acid is heated, thereby dissociating the strands, which in turn are cooled and rebounded. A homoduplex and a heteroduplex are formed when the double stranded nucleic acid is a hetero body, while only a homoduplex is formed when the double stranded nucleic acid is a homo body. The temperature of the solution is increased while measuring ultraviolet absorption, thereby acquiring a thermal melting profile. When the heated and cooled solution contains only a homoduplex, the thermal melting profile has a single Tm temperature or two Tm temperatures close to each other. When the heated and cooled solution contains a homoduplex and a heteroduplex, the thermal melting profile has two separate Tm temperatures or further single Tm temperatures close to each other. The method therefore allows for the detection of a heteroduplex or a mutation in a sample double stranded nucleic acid.

Abstract: An electrode plate of a sample plate is set on the body of an electrophoretic apparatus, while a plug is inserted into a migration high voltage line connection hole and connected to a high-tension distribution cable. Each well of a base plate is inserted into a through hole of a well guide and further press-fit and engaged into a cavity of an electrode plate, for fixing the base plate to the electrode plate. Thereafter a sample is introduced into each well of the base plate and an end of a capillary column is dipped into each well for applying a migration voltage and electrophoretically injecting the sample into the capillary column.

Abstract: A multi-capillary electrophoresis apparatus arranged with a fixed sample injection holder opposite a detection side holder within the same plane and an epi-optical detection system. A sample is injected sequentially and separated components are successively fed to the detection part for analysis by fluorescence. The epi-optical system adjusts the parallelism between the detection side holder and scanning axis of the detector. Thus, a capillary electrophoretic apparatus can detect fluorescence from a fluorochrome bonded to samples as a label without influence by Raman scattering or Rayleigh scattering.

Abstract: A sample is separated and separated components thereof are successively fed to a part to be detected. A laser beam of at least 600 nm from a laser beam source of an optical measuring part is applied to the part to be detected through a dichroic mirror and a lens, for making a fluorochrome bonded to the separated components absorb multiphotons, exciting the fluorochrome and making the same fluoresce. The optical measuring part captures the fluorescence so that photomultipliers detect fluorescence of not more than 510 nm in wavelength, fluorescence longer than 510 nm and not more than 560 nm in wavelength, fluorescence longer than 560 nm and not more than 580 nm in wavelength and fluorescence longer than 580 in wavelength respectively. Thus, a capillary electrophoretic apparatus can detect fluorescence from a fluorochrome bonded to samples as a label without influence by Raman scattering or Rayleigh scattering.

Abstract: Respective monoclonal antibodies are fixed into respective wells of a microplate one by one, and bonded with labeled analogs of PTH amino acid derivatives. A solution containing PTH amino acid derivatives obtained by Edman degradation is dripped on to the microplate for causing competitive reaction to the monoclonal antibodies with the analogs, and thereafter non-bonded PTH amino acid derivatives and liberated analogs are washed out. Then enzyme-labeled antibodies against the analogs are added and the quantity of bonded enzyme-labeled antibodies is measured thereby determining the types and quantities of the PTH amino acid derivatives. Constitutive amino acid can be detected in high sensitivity.

Abstract: A solution containing double stranded nucleic acid is heated for dissociating the double stranded nucleic acid into single strands, which in turn are cooled and rebonded. A homoduplex and a heteroduplex are formed when the double stranded nucleic acid is a hetero body, while only a homoduplex is formed when the double stranded nucleic acid is a homo body. The temperature of the solution is increased for measuring ultraviolet absorption, thereby acquiring a thermal melting profile. When the heated and cooled solution contains only a homoduplex, the thermal melting profile has a single Tm temperature or two Tm temperatures close to each other. When the heated and cooled solution contains a homoduplex and a heteroduplex, the thermal melting profile has two separate Tm temperatures or further single Tm temperatures close to each other.

Abstract: Normal DNA (15) and mutational DNA (17) are mixed and subjected to PCR amplification (A). Fluorescent oligonucleotides (19a, 19b, 19c) complementary to any of exons (15a, 15b, 15c) and labeled with fluorescent materials (F1, F2, F3) having different fluorescence spectral characteristics are prepared (B) and hybridized with the amplified substance for forming homoduplexes (21a, 21b, 21c, 23b) and heteroduplexes (23a, 23c) (C). Since the heteroduplexes (23a, 23c) have a lower melting temperature than the homoduplexes (21a, 21b, 21c, 23b), analysis is made with an ion pair chromatograph having a reversed phase column set at the melting temperature for discriminating and detecting relatively quickly eluting fluorescent oligonucleotides (19a, 19c) thereby detecting mutational exons.

Abstract: A sample containing nucleic acid molecules is mixed with a same volume of reagent such as isopropanol to render the nucleic acid molecules insoluble. This solution containing the nucleic acid molecules in insoluble condition is introduced into a flow cell and exposed therein to light from a light source. Scattered light from the nucleic acid molecules is detected by light sensors and analyzed by a computer.