Patents by Inventor Hideto Chono
Hideto Chono has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220235375Abstract: A method for producing a nucleic acid-encapsulated adeno-associated virus (AAV) hollow particle, comprising the following steps: (1) preparing a linear nucleic acid fragment comprising a sequence for A region and a sequence for D? region in an AAV inverted terminal repeat (ITR) (i.e., an AD sequence) or a sequence complementary to the AD sequence and a target gene sequence; (2) introducing the nucleic acid fragment prepared in step (1) into a cell capable of producing an AAV hollow particle; and (3) culturing the cell obtained in step (2).Type: ApplicationFiled: July 2, 2020Publication date: July 28, 2022Applicants: NIPPON MEDICAL SCHOOL FOUNDATION, TAKARA BIO INC.Inventors: Takashi OKADA, Junichi MINENO, Hideto CHONO
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Patent number: 9850498Abstract: The present invention provides an expression cassette comprising (1) an expression regulatory region having a sequence corresponding to a transcriptional activator-binding region and (2) a nucleic acid encoding a transcriptional activator which is capable of binding to the expression regulatory region, wherein the nucleic acid is operably linked to the expression regulatory region; a method for expressing a target product in a mammalian cell, a method for producing a cell expressing a target product, and a method for producing a target product in a mammalian cell using the expression cassette; and a kit comprising the expression cassette.Type: GrantFiled: December 10, 2013Date of Patent: December 26, 2017Assignee: TAKARA BIO INC.Inventors: Katsuyuki Dodo, Takahisa Tsukihara, Mayumi Shimomura, Koichi Inoue, Hideto Chono, Junichi Mineno, Masanari Kitagawa
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Patent number: 9822380Abstract: A nucleic acid comprising a transcription regulation sequence whose transcription is induced by a trans-acting factor of a human immunodeficiency virus and a gene encoding a polypeptide having an endoribonuclease activity specific to single-stranded RNA, wherein the gene is located in such a position that the expression of the gene can be regulated by the transcription regulation sequence; a method for production of a cell showing an inhibited replication of a human immunodeficiency virus therein, the method comprising the step of introducing the nucleic acid into a cell; and a method for treatment or prevention of a human immunodeficiency virus infection.Type: GrantFiled: October 16, 2014Date of Patent: November 21, 2017Assignee: TAKARA BIO INC.Inventors: Hideto Chono, Kazuya Matsumoto, Junichi Mineno, Ikunoshin Kato
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Patent number: 9670459Abstract: The present invention addresses the problem of providing cell populations having a high proportion of CD4-positive naive T cells and/or CD4-positive central memory T cells, and a production method thereof. The present invention provides a production method for CD4-positive T cell populations which is characterized by using anti-CD3 antibodies, fibronectin fragments, and Interleukin-4. The method is characterized not only by the attainment of a cell group with a high proportion of CD4-positive naive T cells and/or CD4-positive central memory T cells, but also by a high bulk yield.Type: GrantFiled: August 9, 2011Date of Patent: June 6, 2017Assignee: TAKARA BIO INC.Inventors: Katsuyuki Dodo, Hideto Chono, Junichi Mineno
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Patent number: 9228206Abstract: Disclosed is a simple and highly efficient method for introducing a gene into a target cell using a retrovirus vector. The method comprises the steps of (a) placing a liquor containing a retrovirus vector having a foreign gene carried thereon into a bag for cell culture on which a retrovirus-binding substance has been immobilized, and incubating the liquor at a temperature lower than 25° C. for 8-48 hours, thereby producing a culture bag having the retrovirus vector bound thereto, (b) adding a target cell to the culture bag that has been produced in step (a) and incubating the culture bag for 8 hours or less, and (c) flipping the culture bag upside down and incubating the culture bag. The gene introduction method is useful particularly in medicine, cell technology, gene technology, and embryologic technology.Type: GrantFiled: August 12, 2014Date of Patent: January 5, 2016Assignee: TAKARA BIO INC.Inventors: Katsuyuki Dodo, Naoki Saito, Hideto Chono, Junichi Mineno
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Publication number: 20150329872Abstract: The present invention provides an expression cassette comprising (1) an expression regulatory region having a sequence corresponding to a transcriptional activator-binding region and (2) a nucleic acid encoding a transcriptional activator which is capable of binding to the expression regulatory region, wherein the nucleic acid is operably linked to the expression regulatory region; a method for expressing a target product in a mammalian cell, a method for producing a cell expressing a target product, and a method for producing a target product in a mammalian cell using the expression cassette; and a kit comprising the expression cassette.Type: ApplicationFiled: December 10, 2013Publication date: November 19, 2015Applicant: TAKARA BIO INC.Inventors: Katsuyuki DODO, Takahisa TSUKIHARA, Mayurni SHIMOMURA, Koichi INOUE, Hideto CHONO, Junichi MINENO, Masanari KITAGAWA
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Patent number: 9057056Abstract: The N-acetylglucosaminyltransferase III activity is enhanced in a cell carrying retrovirus-origin gag-pol gene and env gene. By constructing a retrovirus vector with the use of the above cell, a retrovirus vector having a modified sugar chain structure can be obtained. The retrovirus vector constructed by this method shows a high infection efficiency particularly in the presence of a functional substance.Type: GrantFiled: September 28, 2005Date of Patent: June 16, 2015Assignee: TAKARA BIO INC.Inventors: Hideto Chono, Hiromi Okuyama, Tomoe Egashira, Nobuto Koyama, Junichi Mineno, Ikunoshin Kato
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Publication number: 20150037300Abstract: A nucleic acid comprising a transcription regulation sequence whose transcription is induced by a trans-acting factor of a human immunodeficiency virus and a gene encoding a polypeptide having an endoribonuclease activity specific to single-stranded RNA, wherein the gene is located in such a position that the expression of the gene can be regulated by the transcription regulation sequence; a method for production of a cell showing an inhibited replication of a human immunodeficiency virus therein, the method comprising the step of introducing the nucleic acid into a cell; and a method for treatment or prevention of a human immunodeficiency virus infection.Type: ApplicationFiled: October 16, 2014Publication date: February 5, 2015Inventors: Hideto CHONO, Kazuya Matsumoto, Junichi Mineno, Ikunoshin Kato
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Publication number: 20140349404Abstract: Disclosed is a simple and highly efficient method for introducing a gene into a target cell using a retrovirus vector. The method comprises the steps of (a) placing a liquor containing a retrovirus vector having a foreign gene carried thereon into a bag for cell culture on which a retrovirus-binding substance has been immobilized, and incubating the liquor at a temperature lower than 25° C. for 8-48 hours, thereby producing a culture bag having the retrovirus vector bound thereto, (b) adding a target cell to the culture bag that has been produced in step (a) and incubating the culture bag for 8 hours or less, and (c) flipping the culture bag upside down and incubating the culture bag. The gene introduction method is useful particularly in medicine, cell technology, gene technology, and embryologic technology.Type: ApplicationFiled: August 12, 2014Publication date: November 27, 2014Inventors: Katsuyuki DODO, Naoki SAITO, Hideto CHONO, Junichi MINENO
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Patent number: 8889844Abstract: A nucleic acid comprising a transcription regulation sequence whose transcription is induced by a trans-acting factor of a human immunodeficiency virus and a gene encoding a polypeptide having an endoribonuclease activity specific to single-stranded RNA, wherein the gene is located in such a position that the expression of the gene can be regulated by the transcription regulation sequence; a method for production of a cell showing an inhibited replication of a human immunodeficiency virus therein, the method comprising the step of introducing the nucleic acid into a cell; and a method for treatment or prevention of a human immunodeficiency virus infection.Type: GrantFiled: August 10, 2006Date of Patent: November 18, 2014Assignee: Takara Bio Inc.Inventors: Hideto Chono, Kazuya Matsumoto, Junichi Mineno, Ikunoshin Kato
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Patent number: 8852915Abstract: A method of retrovirus storage, characterized in that a retrovirus is sustained in the presence of a substance with retrovirus binding activity immobilized on a solid phase. Further, there is provided a retrovirus composition characterized in that a retrovirus in the form of binding to a substance with retrovirus binding activity is sealed in a container holding a solid phase having the substance with retrovirus binding activity immobilized thereon.Type: GrantFiled: October 12, 2012Date of Patent: October 7, 2014Assignee: Takara Bio Inc.Inventors: Hideto Chono, Yasushi Katayama, Hiromi Okuyama, Junichi Mineno, Kiyozo Asada, Ikunoshin Kato
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Patent number: 8841126Abstract: Disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which is simple and highly efficient. Specifically disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which comprises the steps of (a) placing a liquor containing a retrovirus vector having a foreign gene carried thereon into a culture vessel on which a retrovirus-binding substance has been immobilized, and incubating the liquor at a temperature lower than 25° C. for 4 hours or longer, thereby producing a culture vessel having the retrovirus vector bound thereto, and (b) adding a target cell to the culture vessel that has been produced in step (a) and incubating the culture vessel. The gene introduction method is simple and highly efficient, and is useful particularly in the fields of medicine, cell technology, gene technology, embryologic technology and the like.Type: GrantFiled: June 29, 2011Date of Patent: September 23, 2014Assignee: Takara Bio Inc.Inventors: Katsuyuki Dodo, Naoki Saito, Hideto Chono, Junichi Mineno
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Patent number: 8815597Abstract: The present invention provides a baglike container for centrifugation that is mounted in a centrifuge to thereby allow centrifugation of a dispersion liquid accommodated therein. The baglike container for centrifugation is less likely to tear or break as a result of centrifugation by disposing a container wall surface of the baglike container so as to apply centrifugal force perpendicular to the container wall surface.Type: GrantFiled: August 23, 2007Date of Patent: August 26, 2014Assignee: Takara Bio Inc.Inventors: Hideto Chono, Junichi Mineno, Kazutoh Takesako, Takao Yoshida, Takashi Morimura, Kenji Sakai, Shin-ichi Yamada, Noritsugu Yabe, Yuko Taguchi
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Publication number: 20140170709Abstract: The present invention provides a retroviral vector containing a transcription unit comprising a transcription regulatory sequence and a gene encoding a polypeptide having single-stranded RNA-specific endoribonuclease activity which is placed so that its expression can be controlled by the regulatory sequence, wherein the unit is placed so that the direction of the transcription of mRNA from the unit is opposite to the direction of transcription of the RNA genome of the retroviral vector. By using the vector constructed as described above, viral supernatant showing high gene transfer efficiency can be prepared. The retroviral vector of the present invention is useful for the treatment and/or prevention of cancers and virus infections.Type: ApplicationFiled: January 31, 2014Publication date: June 19, 2014Applicant: TAKARA BIO INC.Inventors: Hideto CHONO, Kazuya MATSUMOTO, Hajime MATSUMURA, Junichi MINENO, Ikunoshin KATO
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Publication number: 20130142766Abstract: The present invention addresses the problem of providing cell populations having a high proportion of CD4-positive naive T cells and/or CD4-positive central memory T cells, and a production method thereof. The present invention provides a production method for CD4-positive T cell populations which is characterized by using anti-CD3 antibodies, fibronectin fragments, and Interleukin-4. The method is characterized not only by the attainment of a cell group with a high proportion of CD4-positive naive T cells and/or CD4-positive central memory T cells, but also by a high bulk yield.Type: ApplicationFiled: August 9, 2011Publication date: June 6, 2013Inventors: Katsuyuki Dodo, Hideto Chono, Junichi Mineno
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Publication number: 20130109096Abstract: Disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which is simple and highly efficient. Specifically disclosed is a method for introducing a gene into a target cell using a retrovirus vector, which comprises the steps of (a) placing a liquor containing a retrovirus vector having a foreign gene carried thereon into a culture vessel on which a retrovirus-binding substance has been immobilized, and incubating the liquor at a temperature lower than 25° C. for 4 hours or longer, thereby producing a culture vessel having the retrovirus vector bound thereto, and (b) adding a target cell to the culture vessel that has been produced in step (a) and incubating the culture vessel. The gene introduction method is simple and highly efficient, and is useful particularly in the fields of medicine, cell technology, gene technology, embryologic technology and the like.Type: ApplicationFiled: June 29, 2011Publication date: May 2, 2013Inventors: Katsuyuki Dodo, Naoki Saito, Hideto Chono, Junichi Mineno
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Publication number: 20120142109Abstract: Disclosed is a method for producing a cell population containing memory-like T cells, said method being characterized by including a step for in vitro culturing of a cell population containing T cells or T cell precursor cells using a retinoic acid and a CD3 ligand. Further disclosed is a method for producing a cell population wherein the proportion of memory-like T cells is increased and wherein a desired gene is introduced with a high efficiency and is highly expressed.Type: ApplicationFiled: August 24, 2010Publication date: June 7, 2012Inventors: Yoshinori Katayama, Kensuke Sakai, Hirofumi Yoshioka, Hikaru Takakura, Hideto Chono, Naoki Saito, Junichi Mineno
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Publication number: 20100137415Abstract: The present invention provides a retroviral vector containing a transcription unit comprising a transcription regulatory sequence and a gene encoding a polypeptide having single-stranded RNA-specific endoribonuclease activity which is placed so that its expression can be controlled by the regulatory sequence, wherein the unit is placed so that the direction of the transcription of mRNA from the unit is opposite to the direction of transcription of the RNA genome of the retroviral vector. By using the vector constructed as described above, viral supernatant showing high gene transfer efficiency can be prepared. The retroviral vector of the present invention is useful for the treatment and/or prevention of cancers and virus infections.Type: ApplicationFiled: April 16, 2008Publication date: June 3, 2010Applicant: TAKARA BIO INC.Inventors: Hideto Chono, Kazuya Matsumoto, Hajima Matsumura, Junichi Mineno, Ikunoshin Kato
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Publication number: 20100136680Abstract: Baglike container (1) that is fitted in a centrifuge to thereby allow centrifugation of any dispersion liquid placed therein. The baglike container for centrifugation is one comprising a main frame constituted of first container wall (3) with recess portion (6) formed so as to allow fitting in a centrifuge and with flange portion (7) provided on an open face border of the recess portion (6) and constituted of flexible second container wall (4) having its peripheral portion sealed mutually with the flange portion (7) of the first container wall (3) so as to define storing space (6?) for dispersion liquid in cooperation with the recess portion (6) and further comprising port (5) fitted to the main frame in a fashion allowing communication with the storing space (6?), wherein the recess portion (6) defining the storing space (6?) can be set in the centrifuge so that centrifugal force perpendicular to the bottom plane of the recess portion (6) applies.Type: ApplicationFiled: August 23, 2006Publication date: June 3, 2010Inventors: Hideto Chono, Junichi Mineno, Kazutoh Takesako, Takao Yoshida, Takashi Morimura, Kenji Sakai, Shin-ichi Yamada, Noritsugu Yabe, Yuko Taguchi
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Publication number: 20100113566Abstract: A nucleic acid comprising a transcription regulation sequence whose transcription is induced by a trans-acting factor of a human immunodeficiency virus and a gene encoding a polypeptide having an endoribonuclease activity specific to single-stranded RNA, wherein the gene is located in such a position that the expression of the gene can be regulated by the transcription regulation sequence; a method for production of a cell showing an inhibited replication of a human immunodeficiency virus therein, the method comprising the step of introducing the nucleic acid into a cell; and a method for treatment or prevention of a human immunodeficiency virus infection.Type: ApplicationFiled: August 10, 2006Publication date: May 6, 2010Inventors: Hideto Chono, Kazuya Matsumoto, Junichi Mineno, Ikunoshin Kato