Patents by Inventor Hidetoshi Andou

Hidetoshi Andou has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Process for preparing purified active monomer of bone-derived factor
    Patent number: 7811793
    Abstract: A process for preparing a purified refolded monomer or dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to c) in sequence: a) introducing a polynucleotide encoding a bone morphogenetic factor into a bacterium, expressing said bone morphogenetic factor in the form of an inclusion body, recovering said inclusion body and treating it with a denaturing agent to obtain a solubilized monomer, b) treating the solubilized monomer without purification directly with a refolding solution to obtain a refolded monomeric bone morphogenetic factor, c) subjecting the refolded monomeric bone morphogenetic factor to purification.
    Type: Grant
    Filed: December 15, 2003
    Date of Patent: October 12, 2010
    Assignee: Biopharma Gesellschaft zur Biotechnologischen Entwicklung von Pharmaka MBH
    Inventors: Hidetoshi Andou, Jun Honda, Shunjiro Sugimoto, Gertrud Hötten, Rolf Bechtold, Jens Pohl
  • Process for preparing purified active monomer of bone-derived factor
    Publication number: 20050026247
    Abstract: A process for preparing a purified refolded monomer or dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to c) in sequence: a) introducing a polynucleotide encoding a bone morphogenetic factor into a bacterium, expressing said bone morphogenetic factor in the form of an inclusion body, recovering said inclusion body and treating it with a denaturing agent to obtain a solubilized monomer, b) treating the solubilized monomer without purification directly with a refolding solution to obtain a refolded monomeric bone morphogenetic factor, c) subjecting the refolded monomeric bone morphogenetic factor to purification.
    Type: Application
    Filed: December 15, 2003
    Publication date: February 3, 2005
    Applicant: Biopharma Gesellschaft zur Biotechnologischen Entwicklung von Pharmaka GmbH
    Inventors: Hidetoshi Andou, Jun Honda, Shunjiro Sugimoto, Gertrud Hotten, Rolf Bechtold, Jens Pohl
  • Process for preparing purified active monomer of bone-derived factor
    Publication number: 20040019185
    Abstract: A process for preparing a purified refolded monomer or dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to c) in sequence:
    Type: Application
    Filed: April 16, 2003
    Publication date: January 29, 2004
    Inventors: Hidetoshi Andou, Jun Honda, Shunjiro Sugimoto, Gertrud Hotten, Rolf Bechtold, Jens Pohl
  • Process for preparing purified dimer of bone-derived factor
    Patent number: 6551801
    Abstract: A process for preparing a purified dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to e) in sequence: a) the step of treating an inclusion body of a bone-derived factor with a unfolding agent to prepare a solubilized monomer; b) the step of treating the solubilized monomer with a refolding solution to prepare a dimer; c) the step of subjecting the refolded dimer to ultrafiltration and solvent replacement; d) the step of subjecting the dimer solution prepared above to isoelectric precipitation; and e) the step of subjecting the isoelectrically precipitated dimer to reverse-phase chromatography.
    Type: Grant
    Filed: July 7, 1999
    Date of Patent: April 22, 2003
    Assignee: Biopharm Gesellschaft zur Biotechnologischen Entwicklung von Pharmaka GmbH
    Inventors: Hidetoshi Andou, Jun Honda, Sjunjiro Sugimoto