Abstract: Kits for detecting a mutated target base sequence are provided. For example, the kits can be for detecting a target base sequence (A) containing a nucleotide with a mutated base from a nucleic acid sample. The kits contain a fluorescent-labeled detection probe and a competitive probe combination that is to improve detection by reducing noise. The probe combination is produced using a process that includes, for example, determining the base length and the base sequence of each of the fluorescent-labeled detection probe and the competitive probe. The determining can include experimentally determining the amount to be added to the nucleic acid sample of each of the fluorescent-labeled detection probe and the competitive probe.

Abstract: Methods for designing and producing a fluorescent-labeled detection probe and a competitive probe combination are provided to improve detection by reducing noise. A method for designing the fluorescent-labeled detection probe and a competitive probe combination includes, for example, determining the base length and the base sequence of each of the fluorescent-labeled detection probe and the competitive probe. The determining can include experimentally determining the amount to be added to the nucleic acid sample of each of the fluorescent-labeled detection probe and the competitive probe. The methods provide a functional result of a first order derivative curve for the control target reaction sample having a substantial peak (maximum value), but a first order derivative curve for the control non-target reaction sample not having a substantial peak, the functional result improving detection by reducing noise.

Abstract: The present invention addresses the issue of providing a target base sequence detection method, etc., whereby a determination can be readily made regarding whether or not a target base sequence is present in a nucleic acid sample. A fluorescent-labeled detection probe and a competitive probe are added to a nucleic acid sample and caused to hybridize with the nucleic acid in the sample, the fluorescence intensity is measured while changing the temperature of the reaction sample, and first order differentiation is performed on a temperature-fluorescence intensity curve.

Abstract: The present invention addresses the issue of providing a target base sequence detection method, etc., whereby a determination can be readily made regarding whether or not a target base sequence is present in a nucleic acid sample. A fluorescent-labeled detection probe and a competitive probe are added to a nucleic acid sample and caused to hybridize with the nucleic acid in the sample, the fluorescence intensity is measured while changing the temperature of the reaction sample, and first order differentiation is performed on a temperature-fluorescence intensity curve.

Abstract: The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.

Abstract: A compound container has a container main body forming a first accommodation chamber, an auxiliary container forming a second accommodation chamber, a mounting part on which the auxiliary container is mounted in the container main body, a cutting part which cuts a second accommodation chamber partition wall which partitions a part of the second accommodation chamber formed in the auxiliary container, a contents outlet port in the container main body, and an opening pouring element mounted on the contents outlet port and having another cutting part which cuts a first accommodation chamber partition wall which partitions a part of the first accommodation chamber formed in the container main body.

Abstract: The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.

Abstract: Provided is a container 1 for inspection which stores and pours out contents therein, and the container is provided with a container main body 2 having a mouth portion 22 formed thereon, a cover portion 3 attached to the mouth portion 22 to seal the container main body 2, and an opening cap 4 attached to the cover portion 3. The cover portion 3 has a closing wall 34 for closing the mouth portion 22, and the opening cap 4 is provided with an pouring outlet 421, and a cutting portion 431 for cutting the closing wall 34. When the opening cap 4 is attached to the cover portion 3, the cutting section 431 cuts the closing wall 34 to enable pouring out the contents. Thus, in the provided container 1 for inspection, sealing properties of the container main body 2 are ensured by the cover portion 3, and the closing wall 34 of the cover portion 3 is cut by the opening cap 4 attached to the cover portion 3 without removing the cover portion 3, whereby the contents can be poured out.

Abstract: A compound container which is comprised of a container main body 2 forming a first accommodation chamber 20 and an auxiliary container 3 forming a second accommodation chamber 30, wherein a mounting part 22 on which the auxiliary container 3 is mounted is provided in the container main body 2 and a cutting part 221 which cuts a second accommodation chamber partition wall 30a which partitions part of the second accommodation chamber 30 formed in the auxiliary container 3 is formed, and when the auxiliary container 3 is mounted on the container main body 2, the second accommodation chamber partition wall 30a is cut, whereby the first accommodation chamber 20 and the second accommodation chamber 30 are intercommunicated.

Abstract: The object of the present invention is to provide a method that allows stable amplification of an internal standard material while maintaining an accurate assay value for a target nucleic acid in a nucleic acid detection system involving the use of an internal standard material and a reagent kit used therefor. The present invention relates to a method for nucleic acid amplification comprising preventing an internal standard amplification product from affecting amplification reaction of a target nucleic acid by performing amplification of an internal standard material prior to amplification of the target nucleic acid in the method for amplifying a target nucleic acid in a sample using an internal standard material and a reagent and reagent kit used therefor.

Abstract: Provided is a container 1 for inspection which stores and pours out contents therein, and the container is provided with a container main body 2 having a mouth portion 22 formed thereon, a cover portion 3 attached to the mouth portion 22 to seal the container main body 2, and an opening cap 4 attached to the cover portion 3. The cover portion 3 has a closing wall 34 for closing the mouth portion 22, and the opening cap 4 is provided with an pouring outlet 421, and a cutting portion 431 for cutting the closing wall 34. When the opening cap 4 is attached to the cover portion 3, the cutting section 431 cuts the closing wall 34 to enable pouring out the contents. Thus, in the provided container 1 for inspection, sealing properties of the container main body 2 are ensured by the cover portion 3, and the closing wall 34 of the cover portion 3 is cut by the opening cap 4 attached to the cover portion 3 without removing the cover portion 3, whereby the contents can be poured out.

Abstract: The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3?-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.

Abstract: The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3?-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.

Abstract: The method of the present invention is based on a method wherein a nucleic acid is synthesized utilizing the hybridized 3?-end, which is synthesized by complementary strand synthesis, on a specific region of a target nucleotide sequence existing as the nucleotide sequence of the same strand as the origin for the next round of complementary strand synthesis. The hybridization to a specific region, which region is searched for mutations and polymorphisms, is repeatedly carried out according to the present invention. Thus, mutations and polymorphisms in a target nucleotide sequence are exactly copied to the reaction products.