Patents by Inventor Hideyoshi Harashima
Hideyoshi Harashima has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10092655Abstract: The invention provides a cationic lipid capable of achieving higher intracellular delivery efficiency than conventional cationic lipids, when used as a lipid membrane structure which is a carrier for delivering functional nucleic acid. The cationic lipid is represented by the formula (1): wherein each symbol is as defined herein.Type: GrantFiled: August 7, 2015Date of Patent: October 9, 2018Assignees: NOF CORPORATION, NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Shota Sasaki, Masaki Ota, Kazuhiro Kubo, Hideyoshi Harashima, Hidetaka Akita, Hiroto Hatakeyama, Yuki Noguchi, Kota Tange, Yuta Nakai, Nayuta Shimizu
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Publication number: 20180155304Abstract: The present invention aims to provide a cationic lipid that can be used as a nucleic acid delivery carrier, a lipid membrane structure using a cationic lipid, a nucleic acid-introducing agent using a cationic lipid, and a method of achieving nucleic acid introduction by using a nucleic acid-introducing agent containing a cationic lipid. A lipid membrane structure containing a cationic lipid represented by the formula (1) wherein each symbol is as defined in the DESCRIPTION, is superior in the stability in blood and tumor accumulation property. A nucleic acid-introducing agent using the cationic lipid can achieve high nucleic acid delivery efficiency of nucleic acid to be delivered into the cytoplasm.Type: ApplicationFiled: January 29, 2016Publication date: June 7, 2018Applicants: NOF CORPORATION, NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Yuta NAKAI, Kota TANGE, Hidetaka AKITA, Hideyoshi HARASHIMA, Ryohei TOGASHI, Naoya MIURA, Mio MAETA
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Publication number: 20170274086Abstract: The invention provides a cationic lipid capable of achieving higher intracellular delivery efficiency than conventional cationic lipids, when used as a lipid membrane structure which is a carrier for delivering functional nucleic acid. The cationic lipid is represented by the formula (1): wherein each symbol is as defined herein.Type: ApplicationFiled: August 7, 2015Publication date: September 28, 2017Applicants: NOF CORPORATION, NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Shota SASAKI, Masaki OTA, Kazuhiro KUBO, Hideyoshi HARASHIMA, Hidetaka AKITA, Hiroto HATAKEYAMA, Yuki NOGUCHI, Kota TANGE, Yuta NAKAI, Nayuta SHIMIZU
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Publication number: 20170273905Abstract: A lipid membrane structure encapsulating an siRNA inside thereof and containing a lipid compound of the formula (I) as a lipid component (R1 and R2 represent CH3—(CH2)n—CH?CH—CH2—CH?CH—(CH2)m—, n represents an integer of 3 to 5, m represents an integer of 6 to 10, p represents an integer of 2 to 7, and R3 and R4 represent a C1-4 alkyl group or a C2-4 alkenyl group.Type: ApplicationFiled: May 18, 2015Publication date: September 28, 2017Applicant: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Hideyoshi Harashima, Yusuke Sato, Shota Warashina, Hiroto Hatakeyama, Mamoru Hyodo, Takashi Nakamura
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Patent number: 9717687Abstract: [Problem] To provide: a lipid membrane structure which has such a particle diameter that the lipid membrane structure can be sterilized by filtration, contains a lipid that is bound to a peptide composed of multiple arginine residues as a constituent lipid, and includes a bacterial cell component having dispersibility in a non-polar solvent; and a method for producing a lipid membrane structure which has such a particle diameter that the lipid membrane structure can be sterilized by filtration and includes a substance of interest having dispersibility in a non-polar solvent. [Solution] A lipid membrane structure which has such a particle diameter that the lipid membrane structure can be sterilized by filtration, contains a lipid that is bound to a peptide composed of multiple arginine residues as a constituent lipid, and includes a bacterial cell component having dispersibility in a non-polar solvent.Type: GrantFiled: August 27, 2013Date of Patent: August 1, 2017Assignees: National University Corporation Hokkaido University, Japan BCG Laboratory, University of TsukubaInventors: Hideyoshi Harashima, Takashi Nakamura, Masafumi Fukiage, Megumi Higuchi, Hideyuki Akaza, Jun Miyazaki, Akihiro Nakaya
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Patent number: 9708628Abstract: The present invention relates to a compound represented by the formula (1) wherein Xa and Xb are each independently X1 or X2; s is 1 or 2, R4 is an alkyl group having 1-6 carbon atoms, na and nb are each independently 0 or 1, R1a and R1b are each independently an alkylene group having 1-6 carbon atoms, R2a and R2b are each independently an alkylene group having 1-6 carbon atoms, Ya and Yb are each independently an ester bond, an amide bond, a carbamate bond, an ether bond or a urea bond, and R3a and R3b are each independently a sterol residue, a liposoluble vitamin residue or an aliphatic hydrocarbon group having 12-22 carbon atoms, and use thereof.Type: GrantFiled: May 16, 2014Date of Patent: July 18, 2017Assignees: NOF Corporation, National University Corporation Hokkaido UniversityInventors: Kota Tange, Masaya Arai, Kazuhiro Kubo, Hidetaka Akita, Hideyoshi Harashima, Hiroto Hatakeyama, Ryohei Ishiba, Masami Ukawa, Hiroki Tanaka
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Publication number: 20170056509Abstract: Hyaluronic acid modified with sphingosine-1-phosphate, and a medicine comprising the hyaluronic acid as an active ingredient, wherein the medicine can alleviate hepatic disorder caused by hypoxia/reoxygenation by protecting the liver sinusoidal endothelial cells, and the medicine can prevent and/or treat liver failure due to liver transplantation, hepatectomy, or other surgeries associated with liver ischemia/reperfusion.Type: ApplicationFiled: March 5, 2015Publication date: March 2, 2017Inventors: Hideyoshi HARASHIMA, Mamoru HYODO, Naoyuki TORIYABE, Nobuhiro OHKOHCHI, Takafumi TAMURA, Naoki SANO
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Patent number: 9532950Abstract: The present invention provides a liposome having excellent lung migration ability that had not existed hitherto, by including GALA or Chol-GALA in the liposome. Furthermore, by using the liposome, a pulmonary delivery carrier having a stronger knockdown effect of siRNA when compared to existing carriers is provided.Type: GrantFiled: March 13, 2012Date of Patent: January 3, 2017Assignees: National University Corporation Hokkaido University, Taiho Pharmaceutical Co., Ltd.Inventors: Kenji Kusumoto, Hideyoshi Harashima, Hidetaka Akita, Hiroto Hatakeyama, Taichi Ishitsuka
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Publication number: 20150245997Abstract: [Problem] To provide: a lipid membrane structure which has such a particle diameter that the lipid membrane structure can be sterilized by filtration, contains a lipid that is bound to a peptide composed of multiple arginine residues as a constituent lipid, and includes a bacterial cell component having dispersibility in a non-polar solvent; and a method for producing a lipid membrane structure which has such a particle diameter that the lipid membrane structure can be sterilized by filtration and includes a substance of interest having dispersibility in a non-polar solvent. [Solution] A lipid membrane structure which has such a particle diameter that the lipid membrane structure can be sterilized by filtration, contains a lipid that is bound to a peptide composed of multiple arginine residues as a constituent lipid, and includes a bacterial cell component having dispersibility in a non-polar solvent.Type: ApplicationFiled: August 27, 2013Publication date: September 3, 2015Inventors: Hideyoshi Harashima, Takashi Nakamura, Masafumi Fukiage, Megumi Higuchi, Hideyuki Akaza, Jun Miyazaki, Akihiro Nakaya
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Publication number: 20150202154Abstract: A lipid membrane structure for delivering a substance into a nucleus of a cell, wherein lipid membrane is modified with (a) a polypeptide comprising the amino acid sequence of SEQ ID NO: 1, and/or (b) a polypeptide consisting of an amino acid sequence comprising the amino acid sequence of SEQ ID NO: 1, but including deletion and/or substitution and/or insertion of one or several amino acid residues, and having an activity of promoting migration of the lipid membrane structure into a nucleus of a cell, which can efficiently deliver a nucleic acid into a nucleus of an immunocyte such as dendritic cell.Type: ApplicationFiled: February 9, 2015Publication date: July 23, 2015Applicant: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Hideyoshi HARASHIMA, Hidetaka AKITA, Mohammad Shaheen SHARIF, Takashi NAKAMURA, Soichiro ISHII, Shiroh FUTAKI
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Publication number: 20150140066Abstract: A liposome comprising a lipid covalently bonded with a polyarginine peptide consisting of 4 to 20 continuous arginine residues and a lipid covalently bonded with a GALA peptide consisting of the amino acid sequence of SEQ ID NO: 1 and/or an R-GALA peptide consisting of the amino acid sequence of SEQ ID NO: 2 as component lipids of lipid membrane, and having a lipid membrane in which a protein to be intracellularly delivered is non-covalently bound to outer surface of the membrane, which is for quickly and conveniently deriver a functional protein, especially such a protein having a high molecular weight, into a cell.Type: ApplicationFiled: September 19, 2012Publication date: May 21, 2015Inventors: Hideyoshi Harashima, Yuma Yamada, Sandra Milena Vergara Perez, Yukari Yasuzaki
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Patent number: 8981044Abstract: A lipid membrane structure for delivering a substance into a nucleus of a cell, wherein lipid membrane is modified with (a) a polypeptide comprising the amino acid sequence of SEQ ID NO: 1, and/or (b) a polypeptide consisting of an amino acid sequence comprising the amino acid sequence of SEQ ID NO: 1, but including deletion and/or substitution and/or insertion of one or several amino acid residues, and having an activity of promoting migration of the lipid membrane structure into a nucleus of a cell, which can efficiently deliver a nucleic acid into a nucleus of an immunocyte such as dendritic cell.Type: GrantFiled: April 20, 2011Date of Patent: March 17, 2015Assignee: National University Corporation Hokkaido UniversityInventors: Hideyoshi Harashima, Hidetaka Akita, Mohammad Shaheen Sharif, Takashi Nakamura, Soichiro Ishii, Shiroh Futaki
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Patent number: 8946380Abstract: With the aim of providing a liposome, having a hydrophilic polymer introduced into the outer surface of the liposome membrane, which is a liposome capable of allowing the liposome-entrapped substance to escape from the endosome and be released into the cytoplasm, a liposome membrane component bound to the peptide shown by (a) or (b) below and a liposome membrane component bound to one end of a hydrophilic polymer the other end of which is bound to the peptide shown by (a) or (b) below are included in the liposome: (a) a peptide comprising the amino acid sequence of SEQ ID NO:1; (b) a peptide comprising the amino acid sequence of SEQ ID NO:1 with 1 or more amino acids deleted, replaced or added therein, and capable of fusing lipid membranes with one another under acidic conditions.Type: GrantFiled: January 12, 2007Date of Patent: February 3, 2015Assignee: Japan Science and Technology AgencyInventors: Kentaro Kogure, Shiroh Futaki, Hideyoshi Harashima, Shigetada Chaki
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Patent number: 8920845Abstract: It is intended to provide a method whereby various kinds of microcapsules, which are usable in, for example, a gene vector, can be produced in a small amount. This production method comprises: feeding a fluid containing a substance to be encapsulated into at lease one (13) of material-feeding microchannels in a substrate in which these material-feeding microchannels (12, 13, 14) and a reaction microchannel (18) connected to these material-feeding microchannels are formed; feeding an envelop-forming fluid containing a material for forming envelopes into at least one of the other material-feeding microchannels (12, 14); and allowing the envelope-forming fluid and the fluid containing the encapsulated substance that converge in the reaction microchannel from each material-feeding microchannel to flow through the reaction microchannel while continuously maintaining the interface between the fluids formed at the confluence thereof.Type: GrantFiled: November 2, 2007Date of Patent: December 30, 2014Assignee: National University Corporation Nagoya UniversityInventors: Yoshinobu Baba, Manabu Tokeshi, Noritada Kaji, Hideyoshi Harashima, Kentaro Kogure, Yasuo Shinohara, Hiroshi Kuramoto
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Publication number: 20140335157Abstract: The present invention relates to a compound represented by the formula (1) wherein Xa and Xb are each independently X1 or X2; s is 1 or 2, R4 is an alkyl group having 1-6 carbon atoms, na and nb are each independently 0 or 1, R1a and R1b are each independently an alkylene group having 1-6 carbon atoms, R2a and R2b are each independently an alkylene group having 1-6 carbon atoms, Ya and Yb are each independently an ester bond, an amide bond, a carbamate bond, an ether bond or a urea bond, and R3a and R3b are each independently a sterol residue, a liposoluble vitamin residue or an aliphatic hydrocarbon group having 12-22 carbon atoms, and use thereof.Type: ApplicationFiled: May 16, 2014Publication date: November 13, 2014Inventors: Kota TANGE, Masaya ARAI, Kazuhiro KUBO, Hidetaka AKITA, Hideyoshi HARASHIMA, Hiroto HATAKEYAMA, Ryohei ISHIBA, Masami UKAWA, Hiroki TANAKA
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Patent number: 8809495Abstract: Provided are peptides imparting cell permeability to a lipid membrane structure and/or enhancing the cell permeability of a lipid membrane structure, and a lipid membrane structure which comprises, as a constituent lipid, a lipid bound to such a peptide and has cell permeability or shows enhanced cell permeability. The amino acid sequences of the peptides imparting cell permeability to a lipid membrane structure and/or enhancing the cell permeability of a lipid membrane structure are represented by: LX1X2X1X1X1L, LLX2X1X1X1L and LX1X2X1X1L (wherein L represents a leucine residue; X1 represents a polar amino acid residue; and X2 represents a polar, non-charged and branched chain amino acid residue).Type: GrantFiled: December 14, 2010Date of Patent: August 19, 2014Assignee: National University Corporation Hokkaido UniversityInventors: Hideyoshi Harashima, Takahiro Fujiwara, Hidetaka Akita
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Publication number: 20140079770Abstract: The present invention provides a liposome having excellent lung migration ability that had not existed hitherto, by including GALA or Chol-GALA in the liposome. Furthermore, by using the liposome, a pulmonary delivery carrier having a stronger knockdown effect of siRNA when compared to existing carriers is provided.Type: ApplicationFiled: March 13, 2012Publication date: March 20, 2014Applicants: TAIHO PHARMACEUTICAL CO., LTD., NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Kenji Kusumoto, Hideyoshi Harashima, Hidetaka Akita, Hiroto Hatakeyama, Taichi Ishitsuka
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Publication number: 20130315925Abstract: Methods and compositions for treating or preventing type 2 diabetes by inhibiting expression or activity of monoacylglycerol O-acyltransferase 1 (MOGAT1) are disclosed. Also disclosed are methods to identify such compositions.Type: ApplicationFiled: May 23, 2013Publication date: November 28, 2013Applicant: BIOMEDCORE, INC.Inventors: Hideyoshi HARASHIMA, Yasuhiro HAYASHI, Kazuaki KAJIMOTO
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Patent number: 8592210Abstract: With the object of providing a liposome having cellular and nuclear entry ability, to achieve this object, a liposome is provided having on its surface a peptide comprising multiple consecutive arginine residues, and specifically a liposome is provided wherein the peptide is modified with a hydrophobic group or hydrophobic compound and the hydrophobic group or hydrophobic compound is inserted into a lipid bilayer so that the peptide is exposed on the surface of the bilayer.Type: GrantFiled: March 31, 2006Date of Patent: November 26, 2013Assignee: Japan Science and Technology AgencyInventors: Hideyoshi Harashima, Shiroh Futaki, Kentaro Kogure
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Publication number: 20130195962Abstract: A lipid membrane structure for delivering a substance to a target cell having satisfactory cell permeability, selectivity for target cell, and in vivo stability, wherein lipid membrane is modified with (a) a polyalkylene glycol bound with a target cell-selective ligand; and (b) a polypeptide comprising a plurality of arginine residues.Type: ApplicationFiled: February 23, 2011Publication date: August 1, 2013Applicant: NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Hideyoshi Harashima, Hiroto Hatakeyama, Ikramy Khalil, Kazuhiro Takara