Abstract: A method for producing a brain organoid having an aggregated tau protein including (a) culturing pluripotent stem cells in the presence of a SMAD inhibitor to form an embryoid body, (b) embedding the embryoid body in an extracellular matrix and three-dimensionally culturing the embryoid body in the presence of a SMAD inhibitor and a GSK3? inhibitor to form an organoid that includes neural precursor cells, (c) extracting the organoid from the extracellular matrix and suspension-culturing the organoid in the presence of LIF to form a brain organoid, (d) forcing the brain organoid to express a mutant MAPT gene, and (e) further suspension-culturing the brain organoid after (d) to obtain a brain organoid having an aggregated tau protein.

Abstract: Provided is a packing material for HILIC columns for more accurately and more easily performing oligosaccharide analysis by liquid chromatography; an HILIC column which is filled with the packing material for HILIC columns; and a method for analyzing an oligosaccharide with use of this packing material for HILIC columns A packing material for HILIC columns according to the present invention is composed of particles, each of which is obtained by reacting glycidol to a hydroxyl group of a porous cross-linked polymer base material having the hydroxyl group, and which have a hydrophilicity index of 2.30 or more and a surface-pH index of from 0.95 to 1.05.

Abstract: A liquid chromatography packing material which includes a polymer packing material into which 1.50 mmol or more of carboxyl groups are introduced per 1 g of the packing material, and an index indicating pH of the surface of the polymer packing material as determined by hydrophilic interaction chromatography (HILIC) is 1.30 or more and the index indicating hydrophilicity of the surface of the polymer packing material as determined by a hydrophilic interaction chromatography (HILIC) is from 1.00 to 1.30.

Abstract: A method for forming a dry coating film on a surface of an object to be coated including the steps of: setting a dry coating film thickness T not smaller than 300 ?m; formulating or preparing a colored paint containing a binder resin and a color pigment and satisfying conditions that (a) a scaly pigment having a prescribed shape is further contained, (b) a content of the scaly pigment is from 5 to 45 volume % in components forming the coating film, (c) a coating film having the thickness T hides the surface, and (d) a color difference between the coating film having the thickness T and a coating film having a thickness of 0.7 T is not smaller than 2.0; coating the surface with the colored paint until the surface is hidden; and drying the coating film, as well as a paint used therefor are provided.

Abstract: A liquid chromatography packing material employing crosslinked polymer particles, wherein the volume average particle size of the crosslinked polymer particles is at least 2 ?m but not more than 10 ?m, the amount of fine particles having a particle size of 1 ?m or less among the crosslinked polymer particles is less than 1% by volume, and the identity coefficient in the particle size distribution is not more than 1.15.

Abstract: Provided is a packing material for HILIC columns for more accurately and more easily performing oligosaccharide analysis by liquid chromatography; an HILIC column which is filled with the packing material for HILIC columns; and a method for analyzing an oligosaccharide with use of this packing material for HILIC columns A packing material for HILIC columns according to the present invention is composed of particles, each of which is obtained by reacting glycidol to a hydroxyl group of a porous cross-linked polymer base material having the hydroxyl group, and which have a hydrophilicity index of 2.30 or more and a surface-pH index of from 0.95 to 1.05.

Abstract: A packing material for liquid chromatography, which is excellent in durability, and a column for liquid chromatography, which is filled with the packing material, are provided. The packing material for liquid chromatography is characterized by comprising a hydrophilic resin containing a polyvinyl alcohol resin, to said hydrophilic resin an amino group represented by the formula (1) having been bonded through a spacer. In the formula (1), R1 represents a hydrogen atom or an alkyl group having 1 to 4 carbon atoms, R2 represents an alkyl group having 1 to 6 carbon atoms and having one or more hydroxyl groups, and ? represents a bonding position to the spacer.

Abstract: A liquid chromatographic column includes: a cylindrical column body; an inflow-side filter that is disposed at an eluent inflow-side end of the column body; an outflow-side filter that is disposed at an eluent outflow-side end of the column body; and a filler that is filled between the inflow-side filter and the outflow-side filter, in which the inflow-side filter has a two-layer structure consisting of a first resin filter member and a second resin filter member which are disposed in this order from a side of the filler, and the first resin filter member has an indentation elastic modulus lower than that of the second resin filter member.

Abstract: A mass spectrometry method by a liquid chromatograph-mass spectrometer that can detect an organic acid with high sensitivity by retaining, separating and eluting the organic acid without adding any nonvolatile substance to a mobile phase and without receiving any restriction of a ratio of a water-soluble organic solvent, and an analytical column are provided. The mass spectrometry method is a mass spectrometry method for an organic acid by a liquid chromatograph-mass spectrometer, wherein a column packed with a hydrophilic polymer having an anion-exchange group is used, and as a mobile phase, a water-soluble organic solvent-water mixed solution is used. The analytical column is a column for liquid chromatography for an organic acid, in which a polyether ether ketone (PEEK) resin housing for liquid chromatography is packed with a hydrophilic polymer having an anion-exchange group.

Abstract: A packing material for liquid chromatography, which is excellent in durability, and a column for liquid chromatography, which is filled with the packing material, are provided. The packing material for liquid chromatography is characterized by comprising a hydrophilic resin containing a polyvinyl alcohol resin, to said hydrophilic resin an amino group represented by the formula (1) having been bonded through a spacer. In the formula (1), R1 represents a hydrogen atom or an alkyl group having 1 to 4 carbon atoms, R2 represents an alkyl group having 1 to 6 carbon atoms and having one or more hydroxyl groups, and ? represents a bonding position to the spacer.

Abstract: A method for forming a dry coating film on a surface of an object to be coated including the steps of: setting a dry coating film thickness T not smaller than 300 ?m; formulating or preparing a colored paint containing a binder resin and a color pigment and satisfying conditions that (a) a scaly pigment having a prescribed shape is further contained, (b) a content of the scaly pigment is from 5 to 45 volume % in components forming the coating film, (c) a coating film having the thickness T hides the surface, and (d) a color difference between the coating film having the thickness T and a coating film having a thickness of 0.7 T is not smaller than 2.0; coating the surface with the colored paint until the surface is hidden; and drying the coating film, as well as a paint used therefor are provided.

Abstract: An inspection method is provided for accurate measurement of conductive materials as defects within a silicon oxide film base material embedded in a SOI wafer sample. In the method, the internal state of a sample 2 is inspected by measuring an conductive material within an insulating base material 11 formed upon the sample 2. Ions or electrons are irradiated upon the surface of the inspection region of the base material 11. A surface image is imaged with secondary electrons emitted from the surface 11a and the vicinity of the surface. The inspection region is etched and a surface image is imaged successively with secondary electrons emitted from a surface 11b and from its vicinity, renewed successively at the etched depth. The conductive material within the base material 11 is measured based upon the accumulated surface images.

Abstract: The invention relates to an analysis method which can conduct analysis on low molecular weight compound in a sample containing water-soluble polymer and low molecular weight compound under isocratic conditions without being affected by proteins or the like and in which water-soluble polymer and low molecular weight compound can be separated efficiently and to a column for such an analysis by high performance liquid chromatography, packed with a packing material comprising a crosslinked organic polymer compound obtained by polymerizing glycerin dimethacrylate at 90 mass % or more as starting material, having the exclusion limit molecular weight measured with pullulan of 30000 or less but 3000 or more and having a mass average particle diameter of 0.1 to 100 ?m.

Abstract: A vehicle door handle includes a handle main body and a base member for attaching the handle main body to the vehicle door. The handle main body is lever-shaped, and is supported by the base member to be rotatable in a predetermined range, the handle main body having one of both ends serving as a spindle and the other end serving as a free end, a hook protruding toward the base member in a longitudinal direction of the handle main body formed on the free end of the handle main body. A stopper abutting on the base member formed on a tip end of the hook, and guide walls adjacent to both side surfaces of the stopper, are formed on the base member.

Abstract: The present invention relates to a packing material for ion chromatography, wherein a quaternary ammonium base represented by the following formula (1) is bonded to the substrate directly or through a spacer: wherein R1 represents a group having at least one olefinic double bond or conjugated double bond, each R2 and R3 independently represents an organic residue which may be the same with or different from R1; and separating equipment for chemical substances and a separating method using the packing material. The packing material for ion chromatography, separating equipment for chemical substances and a separating method using the packing material of the present invention, enable to sufficiently separate seven standard inorganic anions and three halogen oxide anions in either of the conductometric detection or ultraviolet detection by postcolumn derivatization while preventing increase in the pressure and imposing no limitations on the measurement temperature or flow rate of mobile phase.

Abstract: A vehicle door handle includes a handle main body arranged at a predetermined position of an exterior of a vehicle door, and operated when the door is opened or closed; and a base member for attaching the handle main body to the vehicle door, wherein the handle main body is lever-shaped, and is supported by the base member to be rotatable in a predetermined range, the handle main body having one of both ends serving as a spindle and the other end serving as a free end, a hook protruding toward the base member in a longitudinal direction of the handle main body is formed on the free end of the handle main body, and a stopper abutting on the base member and restricting a rotation range of the handle main body is formed on a tip end of the hook, thereby setting an operated position and a non-operated position of the handle main body, and guide walls adjacent to both side surfaces of the stopper, respectively, at the non-operated position of the handle main body, are formed on the base member.

Abstract: An inspection method is provided for accurate measurement of conductive materials as defects within a silicon oxide film base material embedded in a SOI wafer sample. In the method, the internal state of a sample 2 is inspected by measuring an conductive material within an insulating base material 11 formed upon the sample 2. Ions or electrons are irradiated upon the surface of the inspection region of the base material 11. A surface image is imaged with secondary electrons emitted from the surface 11a and the vicinity of the surface. The inspection region is etched and a surface image is imaged successively with secondary electrons emitted from a surface 11b and from its vicinity, renewed successively at the etched depth. The conductive material within the base material 11 is measured based upon the accumulated surface images.

Abstract: The invention intends to provide means for producing halogenated aromatic methylamine useful as an intermediate in the production of agrochemical or medical preparations, by an industrially advantageous method. The process according to the present invention for producing halogenated aromatic methylamine is characterized by comprising hydrogen-reducing a halogenated aromatic nitrile represented by formula (1): (wherein X represents a chlorine atom or a fluorine atom, m represents an integer of 1 to 5, n represents an integer of 1 to 5, m+n?6, and when n is 2 or more, each X may be the same or different) using a hydrogenating catalyst in the presence of an organic acid in a solvent to produce a halogenated aromatic methylamine represented by formula (2): (wherein X, m and n have the same meanings as defined above, and a represents an integer of 1 to m).

Abstract: A clad billet 120 has a core material 122 of which outer surface is coated by a coating material 124. A circular front plate 126 is provided at the head of the clad billet 120. The front plate 126 is made of the same material as the coating material 124. As the clad billet 120 is extruded, the front plate 126 first flows out. Accordingly, instead of the core material 122, the front plate 126 forms dead metal. Moreover, since this front plate 126 is made of the same material as the coating material 124, a defective clad such as a three layer clad are not formed. Also, since a billet thrusting face 102a is tapered toward an axis A of a die at an angle of 55-85 degrees, the volume of dead metal itself is reduced, and therefore it is possible to flow out the defective clad, even if it is generated, at an early stage of extrusion.

Abstract: The invention intends to provide means for producing halogenated aromatic methylamine useful as an intermediate in the production of agrochemical or medical preparations, by an industrially advantageous method.