Abstract: A sterilization method for a medical instrument housed in a housing container includes a temperature-increasing step of housing the housing container in the autoclave and introducing high pressure steam into the autoclave to increase a temperature within the autoclave to a prescribed temperature, a cover sheet being thermally bonded to a peripheral wall portion of the container main body; a sterilizing step of holding the temperature within the autoclave at the prescribed temperature for a prescribed time to sterilize a medical instrument; and a cooling step of reducing the temperature within the autoclave. In the temperature-increasing step, the sterilizing step, and the cooling step, compressed gas is introduced into the autoclave under a steam atmosphere obtained by the introduction of the high pressure steam to increase a current pressure in the autoclave to higher than or equal to a pressure in the housing container and inhibit the cover sheet from peeling from the peripheral wall portion.

Abstract: A sterilization method for a medical instrument housed in a housing container includes a temperature-increasing step of housing the housing container in the autoclave and introducing high pressure steam into the autoclave to increase a temperature within the autoclave to a prescribed temperature, a cover sheet being thermally bonded to a peripheral wall portion of the container main body; a sterilizing step of holding the temperature within the autoclave at the prescribed temperature for a prescribed time to sterilize a medical instrument; and a cooling step of reducing the temperature within the autoclave. In the temperature-increasing step, the sterilizing step, and the cooling step, compressed gas is introduced into the autoclave under a steam atmosphere obtained by the introduction of the high pressure steam to increase a current pressure in the autoclave to higher than or equal to a pressure in the housing container and inhibit the cover sheet from peeling from the peripheral wall portion.

Abstract: A carbon dioxide clathrate having a lump diameter enough to precipitate on a desired abyssal position of the sea without being drifted by a seawater flow of the abyss can be produced by bringing an aqueous solution into contact with carbon dioxide with the interposition of an interface in a reactor and retaining this contact; adjusting the pressure in the reactor to 13 atm or more, the temperature of the aqueous solution to a level of more than 5.degree. C. to 10.degree. C., the temperature of the interface to a level of more than 10.degree. C. to 15.degree. C. and the temperature of a lug inserted into the aqueous solution to a level of more than 0.degree. C. to 5.degree. C.; producing/growing the carbon dioxide clathrate on the lug; and then heating the lug to a temperature of more than 10.degree. C. to 30.degree. C. to separate/drop the carbon dioxide clathrate therefrom.