Patents by Inventor Hikaru Takakura
Hikaru Takakura has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10072250Abstract: The present invention provides a method for efficiently manufacturing a non-enveloped virus with high purity without laborious operation by cultivating cells having the ability to produce a non-enveloped virus and bringing the cells and an acidic solution into contact with each other. A non-enveloped virus vector manufactured by the method of the present invention and a composition having the non-enveloped viral vector as an active ingredient are very useful as gene transfer methods in the fields of basic research and clinical application gene therapy.Type: GrantFiled: July 10, 2014Date of Patent: September 11, 2018Assignee: TAKARA BIO INC.Inventors: Shuhei Sakamoto, Yasuhiro Kawano, Katsuyuki Dodo, Tatsuji Enoki, Hirofumi Yoshioka, Hikaru Takakura, Junichi Mineno
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Patent number: 10023846Abstract: Provided are a production method for non-enveloped virus particles which is characterized in that a sample including non-envelopes virus particles is treated with PEG in at least two concentrations; a kit used in said production method; non-enveloped virus particles produced using said production method; and a pharmaceutical composition having the non-envelopes virus particles as an active ingredient.Type: GrantFiled: July 9, 2015Date of Patent: July 17, 2018Assignee: TAKARA BIO INC.Inventors: Toshikazu Nishie, Yasuhiro Kawano, Shuhei Sakamoto, Tatsuji Enoki, Hikaru Takakura, Junichi Mineno
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Publication number: 20170166871Abstract: Provided are a production method for non-enveloped virus particles which is characterized in that a sample including non-envelopes virus particles is treated with PEG in at least two concentrations; a kit used in said production method; non-enveloped virus particles produced using said production method; and a pharmaceutical composition having the non-envelopes virus particles as an active ingredient.Type: ApplicationFiled: July 9, 2015Publication date: June 15, 2017Applicant: TAKARA BIO INC.Inventors: Toshikazu NISHIE, Yasuhiro KAWANO, Shuhei SAKAMOTO, Tatsuji ENOKI, Hikaru TAKAKURA, Junichi MINENO
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Patent number: 9399780Abstract: The present invention provides a method for producing a virus vector, which involves a step of culturing a cell capable of producing the virus vector in a culture medium containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate; and a culture medium for use in the production of a virus vector, which is characterized by containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate as active ingredients.Type: GrantFiled: March 14, 2013Date of Patent: July 26, 2016Assignee: TAKARA BIO INC.Inventors: Kazuhisa Shinmura, Hirofumi Yoshioka, Hikaru Takakura, Junichi Mineno
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Publication number: 20160152955Abstract: The present invention provides a method for efficiently manufacturing a non-enveloped virus with high purity without laborious operation by cultivating cells having the ability to produce a non-enveloped virus and bringing the cells and an acidic solution into contact with each other. A non-enveloped virus vector manufactured by the method of the present invention and a composition having the non-enveloped viral vector as an active ingredient are very useful as gene transfer methods in the fields of basic research and clinical application gene therapy.Type: ApplicationFiled: July 10, 2014Publication date: June 2, 2016Applicant: TAKARA BIO INC.Inventors: Shuhei SAKAMOTO, Yasuhiro KAWANO, Katsuyuki DODO, Tatsuji ENOKI, Hirofumi YOSHIOKA, Hikaru TAKAKURA, Junichi MINENO
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Publication number: 20150079050Abstract: The present invention provides a method for producing a virus vector, which involves a step of culturing a cell capable of producing the virus vector in a culture medium containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate; and a culture medium for use in the production of a virus vector, which is characterized by containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate as active ingredients.Type: ApplicationFiled: March 14, 2013Publication date: March 19, 2015Inventors: Kazuhisa Shinmura, Hirofumi Yoshioka, Hikaru Takakura, Junichi Mineno
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Publication number: 20120142109Abstract: Disclosed is a method for producing a cell population containing memory-like T cells, said method being characterized by including a step for in vitro culturing of a cell population containing T cells or T cell precursor cells using a retinoic acid and a CD3 ligand. Further disclosed is a method for producing a cell population wherein the proportion of memory-like T cells is increased and wherein a desired gene is introduced with a high efficiency and is highly expressed.Type: ApplicationFiled: August 24, 2010Publication date: June 7, 2012Inventors: Yoshinori Katayama, Kensuke Sakai, Hirofumi Yoshioka, Hikaru Takakura, Hideto Chono, Naoki Saito, Junichi Mineno
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Patent number: 8034597Abstract: A polypeptide having an endonuclease activity derived from a psychrophilic microorganism Shewanella sp. strain Ac10, which exhibits high activity at low temperatures, can remove any nucleic acid present in a protein solution and can reduce the viscosity of a protein extract; and a nucleic acid encoding the polypeptide.Type: GrantFiled: March 8, 2006Date of Patent: October 11, 2011Assignee: Takara Bio Inc.Inventors: Naoyuki Awazu, Toshihiro Shodai, Hikaru Takakura, Masanari Kitagawa, Hiroyuki Mukai, Ikunoshin Kato
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Patent number: 8008055Abstract: Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.Type: GrantFiled: August 19, 2008Date of Patent: August 30, 2011Assignee: Takara Bio Inc.Inventors: Takashi Uemori, Yoshimi Sato, Nobuto Koyama, Ryo Hirano, Hikaru Takakura, Hiroshi Kobori, Yuji Hashimoto, Kikyozo Asada, Ikunoshin Kato
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Patent number: 7553946Abstract: Isolated DNAs which are DNAs having a base sequence represented by any of SEQ ID NOS:1 to 6 in Sequence Listing or fragments thereof and showing a stationary phase-specific promoter activity in gram positive bacteria.Type: GrantFiled: August 8, 2006Date of Patent: June 30, 2009Assignee: Takara Bio Inc.Inventors: Tomoko Shimojo, Hikaru Takakura, Kazuyori Ochiai, Kiyozo Asada, Ikunoshin Kato
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Publication number: 20090098600Abstract: Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.Type: ApplicationFiled: August 19, 2008Publication date: April 16, 2009Applicant: TAKARA BIO INC.Inventors: Takashi Uemori, Yoshimi Sato, Nobuto Koyama, Ryo Hirano, Hikaru Takakura, Hiroshi Kobori, Yuji Hashimoto, Kikyozo Asada, Ikunoshin Kato
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Publication number: 20090047705Abstract: A polypeptide having an endonuclease activity derived from a psychrophilic microorganism Shewanella sp. strain AC10, which exhibits high activity at low temperatures, can remove any nucleic acid present in a protein solution and can reduce the viscosity of a protein extract; and a nucleic acid encoding the polypeptide.Type: ApplicationFiled: March 8, 2006Publication date: February 19, 2009Applicant: Takara Bio Inc.Inventors: Naoyuki Awazu, Toshihiro Shodai, Hikaru Takakura, Masanari Kitagawa, Hiroyuki Mukai, Ikunoshin Kato
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Publication number: 20090029416Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.Type: ApplicationFiled: November 27, 2007Publication date: January 29, 2009Applicant: TAKARA BIO INC.Inventors: Hikaru Takakura, Mio Morishita, Katsuhiko Yamamoto, Masanori Mitta, Kiyozo Asada, Susumu Tsunasawa, Ikunoshin Kato
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Patent number: 7422888Abstract: Polypeptides having an RNase H activity highly useful in genetic engineering; genes encoding these polypeptides; and a process for genetic engineeringly producing these polypeptides.Type: GrantFiled: September 13, 2001Date of Patent: September 9, 2008Assignee: Takara Bio Inc.Inventors: Takashi Uemori, Yoshimi Sato, Nobuto Koyama, Ryo Hirano, Hikaru Takakura, Hiroshi Kobori, Yuji Hashimoto, Kiyozo Asada, Ikunoshin Kato
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Publication number: 20080206811Abstract: A process for producing a target protein at low temperature, comprising inducing expression of not only a vector having introduced therein a gene coding for the target protein but also a vector having a chaperone gene introduced therein.Type: ApplicationFiled: August 19, 2005Publication date: August 28, 2008Applicant: Takara Bio Inc.Inventors: Toshihiro Shodai, Hiroshi Kobori, Takehiro Sagara, Hiroshi Endo, Hikaru Takakura, Jun Tomono, Hiroaki Sagawa, Hiroyuki Mukai, Ikunoshin Kato
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Patent number: 7314744Abstract: There are provided hyperthermostable proteases having an amino acid sequences represented by SEQ ID Nos. 1, 3 and 5 of the Sequence Listing or functional equivalents thereof and hyperthermostable protease genes encoding those hyperthermostable protease. There is also disclosed a process for preparation of a hyperthermostable protease by culturing a transformant containing the gene.Type: GrantFiled: March 16, 2004Date of Patent: January 1, 2008Assignee: Takara Bio Inc.Inventors: Hikaru Takakura, Mio Morishita, Katsuhiko Yamamoto, Masanori Mitta, Kiyozo Asada, Susumu Tsunasawa, Ikunoshin Kato
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Patent number: 7144719Abstract: A hyperthermostable protease having the amino acid sequence represented by the SEQ ID NO:1 of the Sequence Listing or a sequence derived therefrom by deletion, substitution, insertion or addition of one to several amino acid residues, a gene encoding the hyperthermostable protease, and a process for preparing the protease, aiming at providing by genetic engineering techniques a hyperthermophile protease which is advantageous for industrial use.Type: GrantFiled: July 12, 2004Date of Patent: December 5, 2006Assignee: Takara Bio, Inc.Inventors: Hikaru Takakura, Mio Morishita, Tomoko Shimojo, Kiyozo Asada, Ikunoshi Kato
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Publication number: 20060269999Abstract: Isolated DNAs which are DNAs having a base sequence represented by any of SEQ ID NOS:1 to 6 in Sequence Listing or fragments thereof and showing a stationary phase-specific promoter activity in gram positive bacteria.Type: ApplicationFiled: August 8, 2006Publication date: November 30, 2006Applicant: TAKARA BIO INC.Inventors: Tomoko Shimojo, Hikaru Takakura, Kazuyori Ochiai, Kiyozo Asada, Ikunoshin Kato
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Patent number: 7125690Abstract: Isolated DNAs which are DNAs having a base sequence represented by any of SEQ ID NOS: 1 to 6 in Sequence Listing or fragments thereof and showing a stationary phase-specific promoter activity in Gram-positive bacteria.Type: GrantFiled: March 13, 2002Date of Patent: October 24, 2006Assignee: Takara Bio Inc.Inventors: Tomoko Shimojo, Hikaru Takakura, Kazuyori Ochiai, Kiyozo Asada, Ikunoshin Kato
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Patent number: 6921656Abstract: Polypeptides having the amino acid sequence represented by SEQ ID NO:6 in Sequence Listing or an amino acid sequence derived from the above sequence by at least one of deletion, addition, insertion or substitution of one or more amino acids and showing a thermophilic glucoamylase activity.Type: GrantFiled: July 26, 2000Date of Patent: July 26, 2005Assignee: Takara Bio Inc.Inventors: Nobuto Koyama, Toshitake Okui, Hikaru Takakura, Kiyozo Asada, Ikunoshin Kato