Patents by Inventor Hilde E. Smith

Hilde E. Smith has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Processing of proteins
    Patent number: 5246838
    Abstract: The subject invention describes the cloning and overexpression of leader peptidase genes. A method for isolating a leader peptidase gene is disclosed. Overexpression of the signal peptidase in a suitable host species leads to an enhanced rate of protein processing.
    Type: Grant
    Filed: February 28, 1991
    Date of Patent: September 21, 1993
    Assignee: Gist-brocades, N.V.
    Inventors: Jan M. Van Dijl, Hilde E. Smith, Sierd Bron, Wilhelmus J. Quax, Gerard Venema
  • Secretory signal selection vectors for extracellular protein synthesis in Bacilli
    Patent number: 5212070
    Abstract: Novel vectors are provided for identifying secretory signal sequences from DNA fragments of unicellular microorganisms. The plasmids comprise a multiple cloning site with restriction sites in reading frame with a structural gene which permits rapid screening of the secreted expression product. Optionally, the vectors may include a promoter region upstream from the multiple cloning site. The invention is exemplified with Bacillus. Specific secretory signal sequences have been isolated with those vectors, allowing for efficient secretion into the supernatant, and not just to the periplasmic space to provide proteins in economically high yields. Secretory sequences are provided superior to other previously known sequences.
    Type: Grant
    Filed: December 13, 1990
    Date of Patent: May 18, 1993
    Assignee: Gist-brocades
    Inventors: Hilde E. Smith, Jan H. Van Ee, Ben P. H. Peeters, Sierd Bron, Gerard Venema
  • Secretory signal selection vectors for extracellular protein synthesis bacilli
    Patent number: 5037760
    Abstract: Novel vectors are provided for identifying secretory signal sequences from DNA fragments of unicellular microorganisms. The plasmids comprise a multiple cloning site with restriction sites in reading frame with a structural gene which permits rapid screening of the screted expression product. Optionally, the vectors may include a promoter region upstream from the multiple cloning site. The invention is exemplified with Bacillus. Specific secretory signal sequence have been isolated with those vectors, allowing for efficient secretion into the supernatant, and not just to the periplasmic space to provide proteins in economically high yields. Secretory sequences are provided superior to other previously known sequences.
    Type: Grant
    Filed: May 1, 1987
    Date of Patent: August 6, 1991
    Assignee: Gist-Brocades NV
    Inventors: Hilde E. Smith, Jan H. Van Ee, Ben P. H. Peeters, Sierd Bron, Gerard Venema