Abstract: A two-component system and device for efficient analysis of Nucleic Acid Amplification Tests (NAAT) on a cartridge that is portable, low cost, delivers rapid results with direct sample application, minimal sample volume, and has a user-friendly interface. The system and device can be used to perform NAAT reactions, either thermal or isothermal and either in single-plex or multi-plex, on a sample within a single-use test cartridge that is integrated for use with a reusable separately housed amplification module and analysis module to generate test results.