Abstract: An image processing method includes: a fluorescent image capturing step of capturing a fluorescent image of a fluorescently labeled tissue specimen; a creating step of creating a fluorescent whole slide image based on the captured fluorescent image; and a storing step of storing the created fluorescent whole slide image. The tissue specimen is fluorescently labelled by using, as a staining reagent, fluorescent substance integrated nanoparticles obtained by bonding a biological substance recognition part to fluorescent particles on which a plurality of fluorescent substances are integrated.

Abstract: An image processing method includes: a fluorescent image capturing step of capturing a fluorescent image of a fluorescently labeled tissue specimen; a creating step of creating a fluorescent whole slide image based on the captured fluorescent image; and a storing step of storing the created fluorescent whole slide image. The tissue specimen is fluorescently labelled by using, as a staining reagent, fluorescent substance integrated nanoparticles obtained by bonding a biological substance recognition part to fluorescent particles on which a plurality of fluorescent substances are integrated.

Abstract: An image processing device includes an inputter, a hardware processor, and a storage. The inputter is to input a morphological image, and a plurality of fluorescent images which having focal planes different at a predetermined interval in a height direction of the tissue sample in a same range as the morphological image and representing expression of the biological substance in the tissue sample with a fluorescent bright spot. The hardware processor extracts a cell region, specifies a focal plane most in focus as an in-focus plane for each cell region, specifies a coordinate in the in-focus plane of the cell region, extracts a fluorescent bright spot region from a fluorescent image in a focal plane corresponding to the in-focus plane, and calculates a luminance value or a number of the fluorescent nanoparticle in the fluorescent bright spot region. The storage stores the in-focus plane and the coordinate.

Abstract: An image processing method includes: a fluorescent image capturing step of capturing a fluorescent image of a fluorescently labeled tissue specimen; a creating step of creating a fluorescent whole slide image based on the captured fluorescent image; and a storing step of storing the created fluorescent whole slide image. The tissue specimen is fluorescently labelled by using, as a staining reagent, fluorescent substance integrated nanoparticles obtained by bonding a biological substance recognition part to fluorescent particles on which a plurality of fluorescent substances are integrated.