Patents by Inventor Hiroaki Udagawa
Hiroaki Udagawa has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20140287475Abstract: The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the filamentous fungal strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein the first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the filamentous fungal strain under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expType: ApplicationFiled: April 17, 2014Publication date: September 25, 2014Applicants: Novozymes A/S, Novozymes, Inc.Inventors: Howard Brody, Suchindra Maiyuran, Hiroaki Udagawa
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Patent number: 8841091Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.Type: GrantFiled: November 2, 2012Date of Patent: September 23, 2014Assignees: Novozymes Als, Novozymec North America Inc.Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen
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Patent number: 8802412Abstract: The present invention relates to isolated polynucleotides having promoter activity the use of the isolated polynucleotides for the production of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.Type: GrantFiled: June 23, 2011Date of Patent: August 12, 2014Assignee: Novozymes A/SInventors: Hiroshi Teramoto, Hiroaki Udagawa, Rikako Taira
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Patent number: 8735563Abstract: The present invention relates to isolated polynucleotides having promoter activity the juse of the isolated polynucleotides for the procuction of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.Type: GrantFiled: June 23, 2011Date of Patent: May 27, 2014Assignee: Novozymes A/SInventors: Hiroshi Teramoto, Hiroaki Udagawa
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Publication number: 20140127753Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.Type: ApplicationFiled: November 2, 2012Publication date: May 8, 2014Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen
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Patent number: 8716023Abstract: The present invention relates to methods for reducing or eliminating the expression of a target gene in a filamentous fungal strain, comprising: (a) inserting into the genome of the filamentous fungal strain a double-stranded transcribable nucleic acid construct comprising a first nucleotide sequence comprising a promoter operably linked to a homologous coding region of the target gene and a second nucleotide sequence comprising the homologous coding region, or a portion thereof, of the target gene, wherein the first and second nucleotide sequences are complementary to each other and the second nucleotide sequence is in reverse orientation relative to the first nucleotide sequence; and (b) inducing production of an interfering RNA encoded by the double-stranded transcribable nucleic acid construct by cultivating the filamentous fungal strain under conditions conducive for production of the interfering RNA; wherein the interfering RNA interacts with RNA transcripts of the target gene to reduce or eliminate expType: GrantFiled: December 9, 2004Date of Patent: May 6, 2014Assignees: Novozymes, Inc., Novozymes A/SInventors: Howard Brody, Suchindra Maiyuran, Hiroaki Udagawa
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Publication number: 20140120625Abstract: The invention relates to a method for the simultaneous integration of two or more copies of a polynucleotide of interest into the chromosome of a fungal host cell comprising at least two pairs of recognition sequences of a site-specific recombinase, each pair flanking a resident negative selection marker; transformation of the cell with a construct carrying a gene of interest also flanked by the recognition sequences to ensure double-crossover events after transient expression of the recombinase, followed by selection for excision of all negative selection markers from the cell.Type: ApplicationFiled: May 23, 2012Publication date: May 1, 2014Inventor: Hiroaki Udagawa
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Publication number: 20140113304Abstract: The present invention relates to a bi-directional cytosine deaminase-encoding selection marker as well as methods for using said marker.Type: ApplicationFiled: May 23, 2012Publication date: April 24, 2014Applicant: Novozymes A/SInventor: Hiroaki Udagawa
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Patent number: 8633010Abstract: The present invention relates to an isolated polypeptide having PepC inhibitory activity as well as to a method for producing a heterologous polypeptide of interest in an Aspergillus host cell comprising: (a) cultivating the Aspergillus host cell comprising a first and a second nucleic acid sequences under conditions conducive for the expression of the polypeptides encoded by the said first and second nucleic acid sequences, and wherein the first nucleic acid sequence encodes a heterologous polypeptide of interest and the second nucleic acid encodes the inhibitor polypeptide of the invention, and wherein the inhibitor polypeptide is expressed from a recombinant nucleic acid construct resulting in an increased level of the inhibitor polypeptide compared to an Aspergillus host cell not comprising the recombinant nucleic acid construct; and (b) recovering the heterologous polypeptide.Type: GrantFiled: December 2, 2008Date of Patent: January 21, 2014Assignee: Novozymes A/SInventors: Jan Lehmbeck, Hiroaki Udagawa
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Patent number: 8512986Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.Type: GrantFiled: December 20, 2010Date of Patent: August 20, 2013Assignees: Novozymes A/S, Novozymes North America, Inc.Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen, Sara Landvik
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Publication number: 20130095525Abstract: The present invention relates to isolated polynucleotides having leader sequence function. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods using the polynucleotides for production of polypeptides.Type: ApplicationFiled: June 23, 2011Publication date: April 18, 2013Applicant: NOVOZYMES A/SInventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi
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Publication number: 20130095550Abstract: The present invention relates to isolated polynucleotides having promoter activity the use of the isolated polynucleotides for the production of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.Type: ApplicationFiled: June 23, 2011Publication date: April 18, 2013Applicant: NOVOZYMES A/SInventors: Hiroshi Teramoto, Hiroaki Udagawa, Rikako Taira
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Publication number: 20130090463Abstract: The present invention relates to isolated polynucleotides having promoter activity the juse of the isolated polynucleotides for the procuction of a polypeptide. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing a desired polypeptide using the polypeptide having promoter activity.Type: ApplicationFiled: June 23, 2011Publication date: April 11, 2013Applicant: NOVOZYMES A/SInventors: Hiroshi Teramoto, Hiroaki Udagawa
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Patent number: 8383391Abstract: This disclosure relates to mutant cells with deleted or disrupted genes encoding protease(s).Type: GrantFiled: September 30, 2008Date of Patent: February 26, 2013Assignee: Novozymes A/SInventors: Hiroaki Udagawa, Christian Isak Jørgensen
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Publication number: 20120294978Abstract: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.Type: ApplicationFiled: July 19, 2012Publication date: November 22, 2012Applicants: NOVOZYMES NORTH AMERICA, INC., NOVOZYMES A/SInventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi, Carsten Hjort, Anders Vikso-Nielson, Eric Allain, Shiro Fukuyama, Tomoko Matsui
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Patent number: 8263381Abstract: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.Type: GrantFiled: August 20, 2010Date of Patent: September 11, 2012Assignees: Novozyms A/S, Novozymes North America, Inc.Inventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi, Carsten Hjort, Anders Vikso-Nielsen, Eric Allain, Shiro Fukuyama, Tomoko Matsui
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Patent number: 8148127Abstract: The present invention relates to polypeptides having glucoamylase activity and isolated polynucleotides encoding said polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. The invention also relates to the composition comprising a glucoamylase of the invention as well as the use such compositions for starch conversion processes, brewing, including processes for producing fermentation products or syrups.Type: GrantFiled: December 10, 2009Date of Patent: April 3, 2012Assignees: Novozymes A/S, Novozymes North America, Inc.Inventors: Hiroaki Udagawa, Sara Landvik, Michiko Ihara, Jiyin Liu, Chee Leong Soong, Eric Allain, Shiro Fukuyama
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Publication number: 20110104759Abstract: The present invention relates to polypeptides comprising a carbohydrate-binding module amino acid sequence and an alpha-amylase amino acid sequence as well as to the application of such polypeptides.Type: ApplicationFiled: December 20, 2010Publication date: May 5, 2011Applicants: Novozymes A/S, Novozymes North America, Inc.Inventors: Shiro Fukuyama, Tomoko Matsui, Chee Leong Soong, Eric Allain, Anders Vikso Nielsen, Hiroaki Udagawa, Ye Liu, Junxin Duan, Wenping Wu, Lene Nonboe Andersen
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Patent number: 7883883Abstract: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.Type: GrantFiled: June 25, 2004Date of Patent: February 8, 2011Assignees: Novozymes A/S, Novozymes North America, Inc.Inventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi, Carsten Hjort, Anders Vikso-Nielsen, Eric Allain, Shiro Fukuyama, Tomoko Matsui
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Publication number: 20100323061Abstract: The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.Type: ApplicationFiled: August 20, 2010Publication date: December 23, 2010Applicants: Novozymes A/S, Novozymes North America, Inc.Inventors: Hiroaki Udagawa, Rikako Taira, Shinobu Takagi, Eric Allain, Carsten Hjort, Anders Vikso-Nielsen, Shiro Fukuyama, Tomoko Matsui