Patents by Inventor Hirobumi Aoki
Hirobumi Aoki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11123656Abstract: The present invention relates to a method of separating and analyzing a mixture of oligonucleotides, including performing liquid chromatography using a column packed with a packing material obtained by fixing a diol to a surface of each of porous particles formed of a crosslinked organic polymer. According to this method, the oligonucleotides can be separated and analyzed with higher sensitivity compared to cases where columns having silica gel as a base material are used. In addition, the column can be washed with an alkaline solution.Type: GrantFiled: June 21, 2018Date of Patent: September 21, 2021Assignee: SHOWA DENKO K.K.Inventors: Junji Sasuga, Hirobumi Aoki, Yuzuru Kokido
-
Publication number: 20200054966Abstract: The present invention relates to a method of separating and analyzing a mixture of oligonucleotides, including performing liquid chromatography using a column packed with a packing material obtained by fixing a diol to a surface of each of porous particles formed of a crosslinked organic polymer. According to this method, the oligonucleotides can be separated and analyzed with higher sensitivity compared to cases where columns having silica gel as a base material are used. In addition, the column can be washed with an alkaline solution.Type: ApplicationFiled: June 21, 2018Publication date: February 20, 2020Applicant: SHOWA DENKO K.K.Inventors: Junji SASUGA, Hirobumi AOKI, Yuzuru KOKIDO
-
Publication number: 20180298052Abstract: An object of the present invention is to obtain a protein monomer useful as a raw material for medicines industrially and economically in high yield and high purity. In the method for purifying a protein of the present invention, a protein solution containing a protein monomer and a protein aggregate is passed through a column holding a porous rigid polymeric self-supporting structure to which a hydrophobic group is immobilized, and then recovering the protein monomer in a flow-through mode.Type: ApplicationFiled: April 28, 2016Publication date: October 18, 2018Applicant: SHOWA DENKO K.K.Inventors: Natsuno MATSUI, Yuzuru KOKIDO, Hirobumi AOKI, Tadashi YONEDA
-
Publication number: 20180079778Abstract: Provided is a method of removing a protein aggregate which can obtain a useful protein monomer in a high yield and a high purity as a raw material of a pharmaceutical product or the like. The method of removing a protein aggregate includes: a step of making the monomer of a protein and the aggregate of proteins adsorb to the column by making a solution containing a monomer of a protein and an aggregate of proteins pass through a column containing a hard porous polymer self-supporting structure to which a strong cation exchange group is fixed; and a step of making a mobile phase consisting of a mixed solution of a buffer solution and an ionic buffer solution pass through the column to which the monomer of a protein and the aggregate of proteins are adsorbed, to selectively elute the monomer of a protein.Type: ApplicationFiled: April 28, 2016Publication date: March 22, 2018Applicant: SHOWA DENKO K.K.Inventors: Yuzuru KOKIDO, Natsuno MATSUI, Hirobumi AOKI, Tadashi YONEDA
-
Patent number: 9677096Abstract: A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequeType: GrantFiled: November 28, 2013Date of Patent: June 13, 2017Assignee: SHOWA DENKO K.K.Inventors: Hirobumi Aoki, Yuzuru Kokido, Yoko Hashimoto, Tadashi Yoneda
-
Patent number: 9434965Abstract: The present invention relates to a method of producing organic acids, comprising biochemical synthesis of organic acid-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of the organic acid-CoA using CoA transferase in the presence of acetic acid, in which organic acid is obtained by culturing transformed microorganisms which have an enzyme gene cluster for the synthesis of organic acid-CoA using acetyl-CoA as a substrate and a CoA transferase gene.Type: GrantFiled: October 10, 2012Date of Patent: September 6, 2016Assignees: SHOWA DENKO K.K., NATIONAL UNIVERSITY CORPORATION HOKKAIDO UNIVERSITYInventors: Ken'ichiro Matsumoto, Seiichi Taguchi, Hirobumi Aoki
-
Publication number: 20150376657Abstract: A method of manufacturing 1,4-butanediol through acetyl-CoA, acetoacetyl-CoA, 3-hydroxybutyryl-CoA, crotonyl-CoA, and 4-hydroxybutyryl-CoA by using a microbe and/or a culture thereof, wherein the microbe in the manufacturing method for 1,4-butanediol includes any one of genes among (a) a gene that has a base sequence of sequence number 1, (b) a gene that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and (c) a gene that hybridizes with a gene that has a base sequence complementary with a gene that has a base sequence described in sequence number 1 on a stringent condition, and includes any one or more genes among (d) genes that have base sequences of sequence numbers 2 to 9, (e) genes that have base sequences such that one or more bases are deleted, substituted, or added in base sequences of sequeType: ApplicationFiled: November 28, 2013Publication date: December 31, 2015Applicant: SHOWA DENKO K. K.Inventors: Hirobumi AOKI, Yuzuru KOKIDO, Yoko HASHIMOTO, Tadashi YONEDA
-
Publication number: 20150353962Abstract: A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acyl-CoA reductase, via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA in this order, wherein the reactivity of the acyl-CoA reductase to 4-hydroxybutyryl CoA is greater than or equal to 0.05 times of the reactivity of the acyl-CoA reductase to 3-hydroxybutyryl-CoA.Type: ApplicationFiled: September 20, 2013Publication date: December 10, 2015Inventors: Hirobumi AOKI, Yuzuru KOKIDO, Yoko HASHIMOTO, Tadashi YONEDA
-
Publication number: 20150307905Abstract: A method of manufacturing a butanediol through 3-hydroxybutyryl-CoA that uses a microbe and/or a culture thereof and utilizes an enzyme reaction that is caused by an acyl-CoA reductase, wherein the microbe in the manufacturing method for a butanediol is characterized in that an activity of an acyl-CoA hydratase (EC number: 3. 1. 2. ?) is deleted or reduced.Type: ApplicationFiled: December 4, 2013Publication date: October 29, 2015Inventors: Hirobumi AOKI, Yuzuru KOKIDO, Yoko HASHIMOTO, Tadashi YONEDA
-
Publication number: 20150291985Abstract: A method of manufacturing 1,4-butanediol by an enzyme reaction system via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA, in this order, using a microbe and/or a culture thereof, wherein the 3-hydroxybutyryl-CoA is an optically active substance, and wherein the microbe includes (1) a gene that codes enoyl-CoA hydratase, (2) a gene that codes vinylacetyl-CoA delta-isomerase, (3) a gene that codes 4-hydroxybutyryl CoA dehydratase, and (4) a gene that codes acyl-CoA reductase whose substrate specificity has an optical selectivity opposite to that of the 3-hydroxybutyryl-CoA.Type: ApplicationFiled: September 20, 2013Publication date: October 15, 2015Inventors: Hirobumi Aoki, Yuzuru Kokido, Yoko Hashimoto, Tadashi Yoneda
-
Publication number: 20150275236Abstract: A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acetoacetyl-CoA reductase and enoyl-CoA hydratase, via acetoacetyl-CoA, 3-hydroxybutyryl-CoA and crotonyl-CoA in this order, wherein each of the acetoacetyl-CoA reductase and the enoyl-CoA hydratase is specific to a stereoisomer of 3-hydroxybutyryl-CoA.Type: ApplicationFiled: September 13, 2013Publication date: October 1, 2015Inventors: Hirobumi Aoki, Yuzuru Kokido, Yoko Hashimoto, Tadashi Yoneda
-
Publication number: 20150252390Abstract: A method of manufacturing a butanediol includes a step of culturing a microbe that contains a gene that codes acetoacetyl-CoA synthase that catalyzes a reaction that irreversibly produces acetoacetyl-CoA from acetyl-CoA and malonyl-CoA, acetoacetyl-CoA reductase that catalyzes a reaction that produces 3-hydroxybutyryl-CoA from acetoacetyl-CoA, and an enzyme that catalyzes a reaction for producing a butanediol from 3-hydroxybutyryl-CoA.Type: ApplicationFiled: August 20, 2013Publication date: September 10, 2015Applicant: SHOWA DENKOInventors: Hirobumi Aoki, Yuzuru Kokido, Tadashi Yoneda
-
Publication number: 20150232830Abstract: A gene is selected from the group consisting of a gene (a) that has a base sequence of sequence number 1, a gene (b) that has a base sequence such that one or more bases are deleted, substituted, or added in a base sequence of sequence number 1, wherein the gene (b) has a base sequence with an identity greater than or equal to 90% with respect to the base sequence of sequence number 1, and a gene (c) that hybridizes with a gene that has a base sequence complimentary with a gene that has a base sequence of sequence number 1 on a stringent condition, wherein the selected gene in combination with a gene that codes enoyl-CoA hydratase is able to provide a microbe or a culture of the microbe with an ability to convert 3-hydroxybutyryl-CoA into 4-hydroxybutyryl-CoA or 4-hydroxybutyryl-CoA into 3-hydroxybutyryl-CoA.Type: ApplicationFiled: September 19, 2013Publication date: August 20, 2015Inventors: Hirobumi Aoki, Yuzuru Kokido, Tadashi Yoneda
-
Publication number: 20140234927Abstract: The present invention relates to a method of producing organic acids, comprising biochemical synthesis of organic acid-CoA using acetyl-CoA as a substrate and conducting CoA elimination reaction of the organic acid-CoA using CoA transferase in the presence of acetic acid, in which organic acid is obtained by culturing transformed microorganisms which have an enzyme gene cluster for the synthesis of organic acid-CoA using acetyl-CoA as a substrate and a CoA transferase gene.Type: ApplicationFiled: October 10, 2012Publication date: August 21, 2014Applicant: SHOWA DENKO K.K.Inventors: Ken'ichiro Matsumoto, Seiichi Taguchi, Hirobumi Aoki
-
Patent number: 8551509Abstract: A skin agent for external use and a cosmetic agent are provided, by transdermal administration of which expected actions and effects of ubiquinone derivatives, salts thereof, ubiquinones and ubiquinols are effectively obtained. The skin agent for external use includes a ubiquinone derivative or a salt thereof as an active ingredient. The ubiquinone derivative is represented by the formula (1): wherein R1 and R2 are each a hydrogen atom or a phosphoric group, at least one of R1 and R2 is a phosphoric group, and n is an integer in the range of 1 to 9.Type: GrantFiled: October 12, 2007Date of Patent: October 8, 2013Assignee: Showa Denko K.K.Inventors: Hirobumi Aoki, Yohei Kurata, Harumi Kamachi, Hirotsugu Nakanishi
-
Patent number: 7943351Abstract: The present invention relates to a method for acyltransferase reaction in which an acyl group of acyl coenzyme A is transferred to an acyl group receptor characterized in that the reaction is carried out by production and/or reproduction of acyl coenzyme A from coenzyme A in a reaction system by a chemical thioester exchange reaction with acylthioester. The present invention, wherein expensive acyl CoA is reproduced nonenzymatically in a reaction system, enables to continuously carry out acyltransferase reaction only by putting a small amount of acyl CoA with a donor and a receptor of an acyl group into a system. Accordingly, the method of the present invention can be applied to an industrial production method of various kinds of compounds including useful biological molecules and synthesis of polymers such as polyester.Type: GrantFiled: November 25, 2008Date of Patent: May 17, 2011Assignee: Showa Denko K.K.Inventors: Motoaki Kamachi, Harumi Kamachi, Hirobumi Aoki, Tomoki Erata, Kenji Tajima
-
Publication number: 20100322887Abstract: It is an object of the present invention to provide a hair nourishing cosmetic which allows an antioxidative action of ubiquinone to contribute to hair nourishment. The hair nourishing cosmetic of the present invention comprises a ubiquinone derivative represented by the following formula (1) and/or its salt, wherein R1 and R2 are each independently a hydrogen atom or a phosphoric acid group, at least one of R1 and R2 is a phosphoric acid group, and n is an integer of 1 to 9.Type: ApplicationFiled: February 6, 2009Publication date: December 23, 2010Applicant: SHOWA DENKO K.K.Inventors: Hirobumi Aoki, Harumi Kamachi
-
Patent number: 7846970Abstract: Hydroxycitric acid derivatives and salts thereof are provided which are useful as active ingredients of skin external preparations and cosmetics. Also provided are processes for production of the hydroxycitric acid derivatives, and skin external preparations and cosmetics containing the hydroxycitric acid derivatives. Specific hydroxycitric acid derivatives or salts thereof are produced wherein at least one of the hydroxyl groups of hydroxycitric acid or least one of the hydroxyl groups and at least one carboxyl group of hydroxycitric acid are modified to linkage moieties breakable by biological enzyme reaction. The hydroxycitric acid derivatives or salts thereof are added in skin external preparations and cosmetics.Type: GrantFiled: September 22, 2005Date of Patent: December 7, 2010Assignee: Showa Denko K.K.Inventors: Harumi Kamachi, Tsuyoshi Katoh, Hirobumi Aoki
-
Patent number: 7842726Abstract: To provide novel carnitine derivatives and salts thereof that are resistant to hydrolysis in the presence of aqueous media, and also provide external skin preparations and cosmetics that are excellent in storage stability, product life, skin affinity and percutaneous absorption properties. In particular, to provide novel ?-branched acyl carnitine derivatives and salts thereof, external skin preparations and cosmetics comprising specific ?-branched acyl carnitine derivatives and/or salts thereof.Type: GrantFiled: September 21, 2006Date of Patent: November 30, 2010Assignee: Showa Denko K.K.Inventors: Hirobumi Aoki, Harumi Kamachi, Yohei Kurata
-
Publication number: 20100280111Abstract: An object of the present invention is to provide skin external preparations and cosmetics which contain a branched acyl carnitine and have excellent formulation stability. A skin external preparation of the present invention includes a carnitine derivative represented by the following Formula (1) and/or a carnitine derivative salt represented by the following Formula (2), and an amphoteric surfactant. In Formula (1), R1 and R2 are each independently a C1-18 optionally branched, saturated or unsaturated aliphatic hydrocarbon group. In Formula (2), R1 and R2 are the same as in Formula (1), X? is a specific anion and Y+ is a specific cation.Type: ApplicationFiled: December 19, 2008Publication date: November 4, 2010Applicant: SHOWA DENKO K.K.Inventors: Hirobumi Aoki, Harumi Kamachi