Patents by Inventor Hirofumi Doi
Hirofumi Doi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11286285Abstract: An object of this invention is to provide a streptavidin mutant reduced in affinity to the naturally-occurring biotin, and to provide a modified biotin which shows a high affinity to such streptavidin mutant reduced in affinity to the naturally-occurring biotin. This invention can provide a compound composed of a dimer of modified biotin, a streptavidin mutant, and usage of them.Type: GrantFiled: February 18, 2015Date of Patent: March 29, 2022Assignee: SAVID THERAPEUTICS INC.Inventors: Akira Sugiyama, Hirofumi Doi, Tatsuhiko Kodama, Tsuyoshi Inoue, Eiichi Mizohata, Tatsuya Kawato, Tomohiro Meshizuka, Motomu Kanai, Yohei Shimizu, Noriaki Takasu, Mari Takatsu
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Patent number: 11161884Abstract: An object of this invention is to provide a streptavidin mutant reduced in affinity to the naturally-occurring biotin, and to provide a modified biotin which shows a high affinity to such streptavidin mutant reduced in affinity to the naturally-occurring biotin. This invention can provide a compound composed of a dimer of modified biotin, a streptavidin mutant, angsd usage of them.Type: GrantFiled: July 16, 2019Date of Patent: November 2, 2021Assignee: SAVID THERAPEUTICS INC.Inventors: Akira Sugiyama, Hirofumi Doi, Tatsuhiko Kodama, Tsuyoshi Inoue, Eiichi Mizohata, Tatsuya Kawato, Tomohiro Meshizuka, Motomu Kanai, Yohei Shimizu, Noriaki Takasu, Mari Takatsu
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Patent number: 11124549Abstract: An object of this invention is to provide a streptavidin mutant reduced in affinity to the naturally-occurring biotin, and to provide a modified biotin which shows a high affinity to such streptavidin mutant reduced in affinity to the naturally-occurring biotin. This invention can provide a compound composed of a dimer of modified biotin, a streptavidin mutant, angsd usage of them.Type: GrantFiled: July 16, 2019Date of Patent: September 21, 2021Assignee: SAVID THERAPEUTICS INC.Inventors: Akira Sugiyama, Hirofumi Doi, Tatsuhiko Kodama, Tsuyoshi Inoue, Eiichi Mizohata, Tatsuya Kawato, Tomohiro Meshizuka, Motomu Kanai, Yohei Shimizu, Noriaki Takasu, Mari Takatsu
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Publication number: 20200017558Abstract: An object of this invention is to provide a streptavidin mutant reduced in affinity to the naturally-occurring biotin, and to provide a modified biotin which shows a high affinity to such streptavidin mutant reduced in affinity to the naturally-occurring biotin. This invention can provide a compound composed of a dimer of modified biotin, a streptavidin mutant, angsd usage of them.Type: ApplicationFiled: July 16, 2019Publication date: January 16, 2020Applicant: SAVID THERAPEUTICS INC.Inventors: Akira SUGIYAMA, Hirofumi DOI, Tatsuhiko KODAMA, Tsuyoshi INOUE, Eiichi MIZOHATA, Tatsuya KAWATO, Tomohiro MESHIZUKA, Motomu KANAI, Yohei SHIMIZU, Noriaki TAKASU, Mari TAKATSU
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Patent number: 9670255Abstract: It is an object of the present invention to provide a mutant streptavidin with a reduced affinity for natural biotin, and also to provide a modified biotin having a high affinity for the mutant streptavidin with a reduced affinity for natural biotin. According to the present invention, there is provided a reagent kit for use in treatments or diagnoses, which comprises: (a) a mutant streptavidin with a reduced affinity for natural biotin or biocytin; and a modified biotin having a high affinity for the above-described mutant streptavidin.Type: GrantFiled: February 18, 2014Date of Patent: June 6, 2017Assignee: SAVID THERAPEUTICS INC.Inventors: Akira Sugiyama, Hirofumi Doi, Tatsuhiko Kodama, Tsuyoshi Inoue, Eiichi Mizohata, Tatsuya Kawato, Tomohiro Meshizuka, Motomu Kanai, Yohei Shimizu, Tomohiro Yamamoto
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Publication number: 20170145063Abstract: An object of this invention is to provide a streptavidin mutant reduced in affinity to the naturally-occurring biotin, and to provide a modified biotin which shows a high affinity to such streptavidin mutant reduced in affinity to the naturally-occurring biotin. This invention can provide a compound composed of a dimer of modified biotin, a streptavidin mutant, and usage of them.Type: ApplicationFiled: February 18, 2015Publication date: May 25, 2017Applicant: SAVID THERAPEUTICS INC.Inventors: Akira SUGIYAMA, Hirofumi DOI, Tatsuhiko KODAMA, Tsuyoshi INOUE, Eiichi MIZOHATA, Tatsuya KAWATO, Tomohiro MESHIZUKA, Motomu KANAI, Yohei SHIMIZU, Noriaki TAKASU, Mari TAKATSU
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Publication number: 20160226355Abstract: A first stator core and a second stator core are configured by arranging pairs of core blocks into an annular shape, the pairs of core blocks being configured by stacking together core blocks so as to be spaced apart axially, the core blocks including circular arc-shaped core back portions and teeth, permanent magnets are each configured so as to be divided into a plurality of magnet blocks that are held between the pairs of core blocks so as to fit inside the pairs of core blocks, and the magnet blocks include a base portion that is held between the core back portions, and that has an external shape in which two circumferential side surfaces are positioned circumferentially inside two circumferential side surfaces of the core back portions.Type: ApplicationFiled: June 18, 2014Publication date: August 4, 2016Applicant: MITSUBISHI ELECTRIC CORPORATIONInventors: Hideaki ARITA, Akihiro DAIKOKU, Hirofumi DOI
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Publication number: 20160137704Abstract: It is an object of the present invention to provide a mutant streptavidin with a reduced affinity for natural biotin, and also to provide a modified biotin having a high affinity for the mutant streptavidin with a reduced affinity for natural biotin. According to the present invention, there is provided a reagent kit for use in treatments or diagnoses, which comprises: (a) a mutant streptavidin with a reduced affinity for natural biotin or biocytin; and a modified biotin having a high affinity for the above-described mutant streptavidin.Type: ApplicationFiled: February 18, 2014Publication date: May 19, 2016Applicant: SAVID THERAPEUTICS INC.Inventors: Akira SUGIYAMA, Hirofumi DOI, Tatsuhiko KODAMA, Tsuyoshi INOUE, Eiichi MIZOHATA, Tatsuya KAWATO, Tomohiro MESHIZUKA, Motomu KANAI, Yohei SHIMIZU, Tomohiro YAMAMOTO
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Patent number: 9051610Abstract: A method for identifying the presence of a target gene mRNA is provided, which involves hybridizing one or more oligonucleic acid probes with the target gene mRNA expressed in a tissue sample, and detecting a low-molecular-weight compound label added to at least one of the bases of the oligonucleic acid probes. The oligonucleic acid probes are contacted with the tissue sample for hybridization with the target gene mRNA after pretreating (prehybridizing) one or more dummy oligonucleic acids with the tissue sample, or a mixture of the oligonucleic acid probes and the dummy oligonucleic acids is contacted with the sample tissue to hybridize the oligonucleic acid probes with the target gene mRNA.Type: GrantFiled: May 19, 2009Date of Patent: June 9, 2015Assignee: CELISH FD, INC.Inventors: Hirofumi Doi, Masahiro Matsuoka, Tatsuhiro Nakano, Chizuru Nagayoshi
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Patent number: 8546537Abstract: It is an object of the present invention to provide a mutant streptavidin wherein the immunogenicity (antigenicity) in mammals of a streptavidin is reduced. The present invention provides a mutant streptavidin, which comprises an amino acid sequence in which (a) the arginine residue at position 72 is substituted with another amino acid residue, and (b) any one or more of the tyrosine residue at position 10, the tyrosine residue at position 71, the glutamic acid residue at position 89, the arginine residue at position 91, and the glutamic acid residue at position 104 are substituted with other amino acid residues, with respect to the amino acid sequence of a core streptavidin as shown in SEQ ID NO: 2, and which has decreased immunogenicity as compared with that of a wild-type streptavidin.Type: GrantFiled: February 19, 2010Date of Patent: October 1, 2013Assignees: The University of Tokyo, Perseus Proteomics Inc.Inventors: Tatsuhiko Kodama, Takao Hamakubo, Hirofumi Doi, Akira Sugiyama, Kouhei Tsumoto
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Publication number: 20120039879Abstract: It is an object of the present invention to provide a mutant streptavidin wherein the immunogenicity (antigenicity) in mammals of a streptavidin is reduced. The present invention provides a mutant streptavidin, which comprises an amino acid sequence in which (a) the arginine residue at position 72 is substituted with another amino acid residue, and (b) any one or more of the tyrosine residue at position 10, the tyrosine residue at position 71, the glutamic acid residue at position 89, the arginine residue at position 91, and the glutamic acid residue at position 104 are substituted with other amino acid residues, with respect to the amino acid sequence of a core streptavidin as shown in SEQ ID NO: 2, and which has decreased immunogenicity as compared with that of a wild-type streptavidin.Type: ApplicationFiled: February 19, 2010Publication date: February 16, 2012Applicants: PERSEUS PROTEOMICS INC., THE UNIVERSITY OF TOKYOInventors: Tatsuhiko Kodama, Takao Hamakubo, Hirofumi Doi, Akira Sugiyama, Kouhei Tsumoto
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Patent number: 8089185Abstract: An actuator having an output axis which is moved linearly by a rotatory force, and a boss for holding the above-mentioned output axis in such a way that the output axis can move linearly includes a connecting member for propagating the linear motion of the above-mentioned output axis to an external device. The above-mentioned connecting member is comprised of a joint connected to the above-mentioned output axis, and a link plate having an end rotatably connected to the above-mentioned joint, and another end rotatably connected to the external device. A mechanism for preventing rotation of the above-mentioned output axis is constructed of only a connecting portion for connecting the above-mentioned joint, the above-mentioned link plate, and the above-mentioned external device to one another.Type: GrantFiled: April 19, 2007Date of Patent: January 3, 2012Assignee: Mitsubishi Electric CorporationInventors: Kenta Hatano, Hirofumi Doi, Toshihiko Miyake
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Patent number: 8003346Abstract: The present invention is to construct a DNA replication reaction system which is excellent in versatility and is easily used. An amino acid sequence of a PCNA monomer which is one of factors involved in DNA replication is prepared so that amino acid residues causing mutual charge repulsion constitute a site which causes, when an N terminal region of the PCNA monomer and a C terminal region of another PCNA monomer act as an interface to form a multimeric complex, an intermolecular interaction of the monomers in an interface region of the monomers.Type: GrantFiled: July 4, 2006Date of Patent: August 23, 2011Assignee: Celestar Lexico-Sciences, Inc.Inventors: Tadaaki Tokida, Satoshi Hihara, Takashi Kudou, Akira Kawamura, Hirofumi Doi, Yoshizumi Ishino
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Patent number: 7990001Abstract: A terminal structure for a vehicle-mounted motor includes a motor body having a motor terminal 28 connected to a coil, and a circuit body 5 holding a circuit board 18 for controlling a rotation of the motor body and is assembled in the motor body, wherein a stress relaxation section 35 is provided in an intermediate of an intermediate conductor 34, in order to reduce stress acting on the intermediate conductor 34 connecting the motor terminal 28 and the circuit board 18 and to thereby improve the conductive reliability of electrical joint.Type: GrantFiled: October 30, 2007Date of Patent: August 2, 2011Assignee: Mitsubishi Electric CorporationInventors: Kenta Hatano, Naoki Miyamoto, Toshiyuki Umemoto, Hirofumi Doi
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Publication number: 20110183331Abstract: Disclosed is a method for identifying the presence of a target gene mRNA, which comprises hybridizing one or more oligonucleic acid probes with the target gene mRNA expressed in a tissue sample, and detecting a low-molecular-weight compound label added to at least one of the bases of the oligonucleic acid probes, wherein the oligonucleic acid probes are contacted with the tissue sample for hybridization with the target gene mRNA after pretreating (prehybridizing) one or more dummy oligonucleic acids with the tissue sample, or a mixture of the oligonucleic acid probes and the dummy oligonucleic acids is contacted with the sample tissue to hybridize the oligonucleic acid probes with the target gene mRNA, and wherein the dummy oligonucleic acids are substantially equal in length with the oligonucleic acid probes, and are neither hybridizable with regions of the target gene mRNA with which the oligonucleic acid probes hybridize nor with the oligonucleic acid probes.Type: ApplicationFiled: May 19, 2009Publication date: July 28, 2011Applicant: Celish FD, INC.Inventors: Hirofumi Doi, Masahiro Matsuoka, Tatsuhiro Nakano, Chizuru Nagayoshi
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Patent number: 7820423Abstract: The present application provides a method for predicting the functional site of a protein using data of the entire proteins of an organism of which genome data or cDNA data is known. More specifically, the present application provides a method for predicting a protein functional site, comprising the steps of calculating the frequency of occurrence of an oligopeptide in the entire proteins, calculating the value of each amino-acid residue contributing to the frequency of occurrence as the representative value of the function, and predicting the protein functional site by using the representative value of function as an indicator. The present also provides a system for predicting a functional site for automatically performing said methods.Type: GrantFiled: May 31, 2007Date of Patent: October 26, 2010Assignee: Japan Science And Technology CorporationInventors: Hirofumi Doi, Hideaki Hiraki, Akio Kanai
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Publication number: 20100230907Abstract: An actuator equipped with a boot for preventing an intruder from intruding into the actuator by way of an output axis's surface includes a plate formed in a cylindrical shape, having an end in which a flange to which an end portion of the above-mentioned boot is locked is formed and being fitted to the above-mentioned output axis, and a projection formed on an outer surface of the above-mentioned output axis in such away as to lock the above-mentioned plate. A breathing hole for communicating the interior of the above-mentioned boot with the exterior of the above-mentioned boot is disposed between the above-mentioned output axis and the above-mentioned plate.Type: ApplicationFiled: April 19, 2007Publication date: September 16, 2010Inventors: Kenta Hatano, Hirofumi Doi, Toshihiko Miyake
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Publication number: 20100231070Abstract: An actuator having an output axis which is moved linearly by a rotatory force, and a boss for holding the above-mentioned output axis in such a way that the output axis can move linearly includes a connecting member for propagating the linear motion of the above-mentioned output axis to an external device. The above-mentioned connecting member is comprised of a joint connected to the above-mentioned output axis, and a link plate having an end rotatably connected to the above-mentioned joint, and another end rotatably connected to the external device. A mechanism for preventing rotation of the above-mentioned output axis is constructed of only a connecting portion for connecting the above-mentioned joint, the above-mentioned link plate, and the above-mentioned external device to one another.Type: ApplicationFiled: April 19, 2007Publication date: September 16, 2010Inventors: Kenta Hatano, Hirofumi Doi, Toshihiko Miyake
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Publication number: 20100071435Abstract: A method of producing a heat shield includes the step of producing a plate-shaped material provided by developing a funnel shape having a plurality of face sections (20, 21, 22) which are adjacent to each other and one (20) of which is a mounting surface and the bending step of bending the plate-shaped material such that the face sections (21, 22) other than the face section (20) corresponding to the mounting surface are orthogonal to the mounting surface section.Type: ApplicationFiled: December 26, 2007Publication date: March 25, 2010Inventors: Kenta Hatano, Toshiyuki Umemoto, Hirofumi Doi
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Publication number: 20100007228Abstract: A terminal structure for a vehicle-mounted motor includes a motor body having a motor terminal 28 connected to a coil, and a circuit body 5 holding a circuit board 18 for controlling a rotation of the motor body and is assembled in the motor body, wherein a stress relaxation section 35 is provided in an intermediate of an intermediate conductor 34, in order to reduce stress acting on the intermediate conductor 34 connecting the motor terminal 28 and the circuit board 18 and to thereby improve the conductive reliability of electrical joint.Type: ApplicationFiled: October 30, 2007Publication date: January 14, 2010Inventors: Kenta Hatano, Naoki Miyamoto, Toshiyuki Umemoto, Hirofumi Doi