Abstract: Devices and methods for preparing RNA and DNA from single cells are disclosed. In particular, the invention relates to a method of simultaneously extracting RNA and DNA from single cells and separating the nucleic acids electrophoretically. An electric field is used to lyse a single target cell, such that the plasma membrane is selectively disrupted without lysing the nuclear membrane. Cytoplasmic RNA is separated from the nucleus by performing isotachophoresis (ITP) in the presence of a sieving matrix that preferentially reduces the mobility of the nucleus. During ITP, the cytoplasmic RNA accumulates at an ITP interface between leading and trailing electrolytes and can later be extracted free of nuclear DNA. The method can be performed in a microfluidic device that fully automates all steps of the process.

Abstract: An artificial sensory epithelium (1) to be embedded in a cochlea (301) of a patient includes a piezoelectric film (2) and multitude microelectrodes (3), (3), . . . arranged on the cochlea. The piezoelectric film is arranged along a basilar membrane (306) in the cochlea. A portion of the piezoelectric film that corresponds to a portion of the basilar membrane that is induced to vibrate by sound waves coming from outside the cochlea vibrates. A microelectrode of the microelectrodes that is arranged on the portion of the piezoelectric film provides electric stimuli to spiral ganglion neurons (311). The artificial sensory epithelium preferably includes a support member (4) configured to support the piezoelectric film and be fixable in the cochlea.

Abstract: An artificial sensory epithelium (1) to be embedded in a cochlea (301) of a patient includes a piezoelectric film (2) and multitude microelectrodes (3), (3), . . . arranged on the cochlea. The piezoelectric film is arranged along a basilar membrane (306) in the cochlea. A portion of the piezoelectric film that corresponds to a portion of the basilar membrane that is induced to vibrate by sound waves coming from outside the cochlea vibrates. A microelectrode of the microelectrodes that is arranged on the portion of the piezoelectric film provides electric stimuli to spiral ganglion neurons (311). The artificial sensory epithelium preferably includes a support member (4) configured to support the piezoelectric film and be fixable in the cochlea.

Abstract: Devices and methods for preparing RNA and DNA from single cells are disclosed. In particular, the invention relates to a method of simultaneously extracting RNA and DNA from single cells and separating the nucleic acids electrophoretically. An electric field is used to lyse a single target cell, such that the plasma membrane is selectively disrupted without lysing the nuclear membrane. Cytoplasmic RNA is separated from the nucleus by performing isotachophoresis (ITP) in the presence of a sieving matrix that preferentially reduces the mobility of the nucleus. During ITP, the cytoplasmic RNA accumulates at an ITP interface between leading and trailing electrolytes and can later be extracted free of nuclear DNA. The method can be performed in a microfluidic device that fully automates all steps of the process.