Patents by Inventor Hirofumi Shiono

Hirofumi Shiono has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11192106
    Abstract: A fluidic device includes a first circulation flow path and a second circulation flow path which circulate a solution containing a sample material, the first circulation flow path and the second circulation flow path share at least a part of the flow path, and at least one selected from the group consisting of a capture unit which captures the sample material, a detection unit which detects the sample material, a valve, and a pump is provided on the shared flow path.
    Type: Grant
    Filed: December 4, 2018
    Date of Patent: December 7, 2021
    Assignee: Nikon Corporation
    Inventors: Ryo Kobayashi, Keiji Mitsui, Taro Ueno, Hirofumi Shiono
  • Publication number: 20210348992
    Abstract: The present invention provides a fluidic device in which solutions of different concentrations can be easily obtained.
    Type: Application
    Filed: June 29, 2018
    Publication date: November 11, 2021
    Inventors: Ryo KOBAYASHI, Hirofumi SHIONO
  • Patent number: 10775368
    Abstract: A fluidic device includes: a circulation flow path; and a capture part arranged on the circulation flow path and configured to capture a sample substance in a solution and/or a detection part arranged on the circulation flow path and configured to detect a sample substance in a solution.
    Type: Grant
    Filed: August 17, 2017
    Date of Patent: September 15, 2020
    Assignees: The University of Tokyo, NIKON CORPORATION
    Inventors: Takanori Ichiki, Taro Ueno, Ryo Kobayashi, Hirofumi Shiono
  • Patent number: 10385305
    Abstract: A device for measuring activity of cultured cells includes a position detecting unit specifying a position of a cell to be measured, a microchamber controlling unit disposing in the culture container a microchamber which surrounds the cell and forms a measurement space, the measurement space being minute with respect to a volume of the culture container, and a measuring unit measuring environmental factors contained in the measurement space.
    Type: Grant
    Filed: March 9, 2015
    Date of Patent: August 20, 2019
    Assignees: THE UNIVERSITY OF TOKYO, NIKON CORPORATION
    Inventors: Takanori Ichiki, Hirofumi Shiono
  • Patent number: 10294472
    Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5?-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3?-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.
    Type: Grant
    Filed: September 10, 2015
    Date of Patent: May 21, 2019
    Assignees: THE UNIVERSITY OF TOKYO, NIKON CORPORATION
    Inventors: Takanori Ichiki, Shingo Ueno, Manish Biyani, Ryo Kobayashi, Hirofumi Shiono
  • Publication number: 20190111429
    Abstract: A fluidic device includes a first circulation flow path and a second circulation flow path which circulate a solution containing a sample material, the first circulation flow path and the second circulation flow path share at least a part of the flow path, and at least one selected from the group consisting of a capture unit which captures the sample material, a detection unit which detects the sample material, a valve, and a pump is provided on the shared flow path.
    Type: Application
    Filed: December 4, 2018
    Publication date: April 18, 2019
    Inventors: Ryo KOBAYASHI, Keiji Mitsui, Taro Ueno, Hirofumi Shiono
  • Patent number: 9952215
    Abstract: A method of analyzing an exosome is provided for detecting abnormality in a cell, including: (a) a step of preparing an exosome from a sample; (b) a step of bringing the exosome prepared in the step (a) into contact with a first antibody to a protein which exists on the surface of the exosome as an antigen and forming a first antibody-exosome complex; and (c) a step of measuring a zeta potential of the first antibody-exosome complex.
    Type: Grant
    Filed: February 23, 2015
    Date of Patent: April 24, 2018
    Assignees: The University of Tokyo, Nikon Corporation
    Inventors: Takanori Ichiki, Takanori Akagi, Hirofumi Shiono
  • Publication number: 20180080076
    Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5? protruding end or a 3? protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of
    Type: Application
    Filed: November 16, 2017
    Publication date: March 22, 2018
    Applicants: The University of Tokyo, Nikon Corporation
    Inventors: Taro Ueno, Takashi Funatsu, Takanori Ichiki, Hirofumi Shiono
  • Publication number: 20180001320
    Abstract: A fluidic device includes: a first flow path in which two or more solutions are mixed; and a second circulation flow path in which a solution mixed in the first flow path is circulated and which has a capture part configured to capture a sample substance included in the solution and/or a detection part configured to detect a sample substance included in the solution.
    Type: Application
    Filed: August 24, 2017
    Publication date: January 4, 2018
    Inventors: Takanori ICHIKI, Taro UENO, Ryo KOBAYASHI, Hirofumi SHIONO
  • Publication number: 20170370922
    Abstract: A fluidic device includes: a circulation flow path; and a capture part arranged on the circulation flow path and configured to capture a sample substance in a solution and/or a detection part arranged on the circulation flow path and configured to detect a sample substance in a solution.
    Type: Application
    Filed: August 17, 2017
    Publication date: December 28, 2017
    Inventors: Takanori ICHIKI, Taro UENO, Ryo KOBAYASHI, Hirofumi SHIONO
  • Patent number: 9850535
    Abstract: A method for detecting a target nucleic acid, comprising: (a) contacting a nucleic acid sample comprising a target nucleic acid, comprising a first portion and a second portion, with: (i) a detection probe, wherein the detection probe is labeled with a labeling substance and comprises a nucleic acid sequence that forms a stem-loop structure and having a 5? protruding end or a 3? protruding end that is capable of hybridizing to the second portion, and (ii) a capture probe comprising a nucleic acid sequence capable of hybridizing to the first portion, wherein the capture probe is immobilized to a substrate, under conditions to form a target nucleic acid-detection probe-capture probe complex by hybridizing the second portion to the detection probe and hybridizing the first portion to the capture probe; (b) ligating a first end of the detection probe with an end of the target nucleic acid and ligating a second end of the detection probe with an end of the capture probe; and (c) detecting the labeling substance of
    Type: Grant
    Filed: October 9, 2014
    Date of Patent: December 26, 2017
    Assignees: The University of Tokyo, Nikon Corporation
    Inventors: Taro Ueno, Takashi Funatsu, Takanori Ichiki, Hirofumi Shiono
  • Patent number: 9844764
    Abstract: The present invention relates to a protein or peptide printing method, comprising (a) a step for preparing nucleic acids and a cell-free protein synthesis system in an engraved plate composed of microscopic grooves having a specific opening shape, (b) a step for superimposing a substrate on the engraved plate so as to contact a protein or peptide to be synthesized in the microscopic grooves, and (c) a step for synthesizing the protein or peptide from the nucleic acids using the cell-free protein synthesis system in the microscopic grooves, and immobilizing the protein or peptide on the substrate along the specific opening shapes of the microscopic grooves.
    Type: Grant
    Filed: February 21, 2013
    Date of Patent: December 19, 2017
    Assignees: University of Tokyo, Nikon Corporation
    Inventors: Takanori Ichiki, Manish Biyani, Hirofumi Shiono
  • Publication number: 20160319236
    Abstract: A culture device containing a culture vessel for culturing a cell, in which the culture device includes a thermostatic vessel having a stacker containing a plurality of culture vessels for culturing a cell, and an observation portion arranged to be isolated from an atmosphere at inside of the thermostatic vessel and having an object lens, an object lens drive portion, and a camera portion to observe a cell at inside of the culture vessel installed at an observation position at inside of the thermostatic vessel.
    Type: Application
    Filed: May 23, 2016
    Publication date: November 3, 2016
    Applicant: NIKON CORPORATION
    Inventors: Yasujiro KIYOTA, Takayuki UOZUMI, Hirofumi SHIONO, Nobuhiko MAIYA
  • Publication number: 20160298070
    Abstract: A transfer device for a culture vessel for transferring a culture vessel for culturing a cell, a culture device including the transfer device for a culture vessel, and a holder for a culture vessel for holding a culture vessel, in which the transfer device for a culture vessel includes a transferring unit transferring a culture vessel for culturing a cell, an inputting unit inputting a kind of the culture vessel, a speed setting unit setting a transfer speed of the culture vessel based on a kind of the culture vessel inputted to the inputting unit, and a controlling unit controlling a transfer speed of the transferring unit to be the transfer speed set by the speed setting unit.
    Type: Application
    Filed: April 12, 2016
    Publication date: October 13, 2016
    Applicant: NIKON CORPORATION
    Inventors: Takayuki UOZUMI, Yasujiro KIYOTA, Nobuhiko MAIYA, Hirofumi SHIONO
  • Patent number: 9376657
    Abstract: A culture device containing a culture vessel for culturing a cell, in which the culture device includes a thermostatic vessel having a stacker containing a plurality of culture vessels for culturing a cell, and an observation portion arranged to be isolated from an atmosphere at inside of the thermostatic vessel and having an object lens, an object lens drive portion, and a camera portion to observe a cell at inside of the culture vessel installed at an observation position at inside of the thermostatic vessel.
    Type: Grant
    Filed: June 7, 2006
    Date of Patent: June 28, 2016
    Assignee: NIKON CORPORATION
    Inventors: Yasujiro Kiyota, Takayuki Uozumi, Hirofumi Shiono, Nobuhiko Maiya
  • Patent number: 9340764
    Abstract: A transfer device for a culture vessel for transferring a culture vessel for culturing a cell, a culture device including the transfer device for a culture vessel, and a holder for a culture vessel for holding a culture vessel, in which the transfer device for a culture vessel includes a transferring unit transferring a culture vessel for culturing a cell, an inputting unit inputting a kind of the culture vessel, a speed setting unit setting a transfer speed of the culture vessel based on a kind of the culture vessel inputted to the inputting unit, and a controlling unit controlling a transfer speed of the transferring unit to be the transfer speed set by the speed setting unit.
    Type: Grant
    Filed: June 27, 2006
    Date of Patent: May 17, 2016
    Assignee: NIKON CORPORATION
    Inventors: Takayuki Uozumi, Yasujiro Kiyota, Nobuhiko Maiya, Hirofumi Shiono
  • Patent number: 9334492
    Abstract: A protein-immobilizing solid phase is a protein-immobilizing solid phase comprising an mRNA-nucleic acid linker-protein complex, obtained by linking the mRNA and the protein encoded by that mRNA through the nucleic acid linker, immobilized on the solid phase, wherein the nucleic acid linker has a photocleavage site and a solid phase binding site.
    Type: Grant
    Filed: April 30, 2014
    Date of Patent: May 10, 2016
    Assignees: The University of Tokyo, NIKON CORPORATION
    Inventors: Shingo Ueno, Naoto Nemoto, Takanori Ichiki, Hirofumi Shiono, Hisao Osawa
  • Publication number: 20160076022
    Abstract: The present invention relates to a nucleic acid linker for producing a complex of mRNA, and a protein or a peptide which is encoded by the mRNA, the linker comprising: a spacer portion at the 5?-terminal; a polynucleotide portion hybridizable with at least a part of a sequence of the mRNA; and an arm portion which has a connection portion for the protein or the peptide at the 3?-terminal, in which the spacer portion, the polynucleotide portion, and the arm portion form a single strand, and in which the polynucleotide portion contains a photoreactive base derivative.
    Type: Application
    Filed: September 10, 2015
    Publication date: March 17, 2016
    Applicants: The University of Tokyo, Nikon Corporation
    Inventors: Takanori Ichiki, Shingo Ueno, Manish Biyani, Ryo Kobayashi, Hirofumi Shiono
  • Publication number: 20150225688
    Abstract: A device for measuring activity of cultured cells includes a position detecting unit specifying a position of a cell to be measured, a microchamber controlling unit disposing in the culture container a microchamber which surrounds the cell and forms a measurement space, the measurement space being minute with respect to a volume of the culture container, and a measuring unit measuring environmental factors contained in the measurement space.
    Type: Application
    Filed: March 9, 2015
    Publication date: August 13, 2015
    Inventors: Takanori ICHIKI, Hirofumi SHIONO
  • Publication number: 20150168400
    Abstract: A method of analyzing an exosome is provided for detecting abnormality in a cell, including: (a) a step of preparing an exosome from a sample; (b) a step of bringing the exosome prepared in the step (a) into contact with a first antibody to a protein which exists on the surface of the exosome as an antigen and forming a first antibody-exosome complex; and (c) a step of measuring a zeta potential of the first antibody-exosome complex.
    Type: Application
    Filed: February 23, 2015
    Publication date: June 18, 2015
    Applicants: The University of Tokyo, NIKON CORPORATION
    Inventors: Takanori ICHIKI, Takanori Akagi, Hirofumi Shiono