Patents by Inventor Hirofumi Yamano

Hirofumi Yamano has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200190595
    Abstract: The present invention easily identifies a genotype for gene mutations related to myeloproliferative neoplasms. The present invention comprises a mutant probe that specifically hybridizes with a gene mutation related to myeloproliferative neoplasms in JAK2, a mutant probe that specifically hybridizes with a gene mutation related to myeloproliferative neoplasms in CALR, and a mutant probe that specifically hybridizes with a gene mutation related to myeloproliferative neoplasms in MPL.
    Type: Application
    Filed: June 28, 2018
    Publication date: June 18, 2020
    Applicants: Toyo Kohan Co., Ltd., Yamaguchi University
    Inventors: Emi Takamitsu, Mitsuyoshi Oba, Junichi Morihiro, Hirofumi Yamano, Toshiaki Yujiri, Masafumi Matsuguma
  • Publication number: 20190161791
    Abstract: In the case of using a blocking nucleic acid to prevent non-specific hybridization of a target nucleic acid with a nucleic acid probe, further excellent efficiency of detecting the target nucleic acid is achieved. A buffer composition used in hybridization of a target nucleic acid with a nucleic acid probe, wherein the buffer composition for hybridization contains a blocking nucleic acid comprising a nucleotide sequence complementary to a region comprising at least a non-detection target nucleotide in a non-target nucleic acid, in a concentration of one or more times higher than the concentration of a nucleic acid in a nucleic acid mixture consisting of the target nucleic acid and the non-target nucleic acid.
    Type: Application
    Filed: August 2, 2017
    Publication date: May 30, 2019
    Applicants: TOYO KOHAN CO., LTD., YAMAGUCHI UNIVERSITY
    Inventors: Toshiya TSUDA, Shuichi KAMEI, Mitsuyoshi OBA, Hirofumi YAMANO, Ryouichi TSUNEDOMI, Shoichi HAZAMA, Hiroaki NAGANO
  • Publication number: 20170253932
    Abstract: An object of the present invention is to detect a mutation related to BCR-ABL inhibitor resistance with high accuracy for chronic myelogenous leukemia or the like. A method for detecting the mutation comprises the steps of: amplifying a region including a fusion site and a mutation site related to BCR-ABL inhibitor resistance contained in a BCR-ABL fusion gene using a biological sample collected from a subject; and genotyping of the mutation site related to BCR-ABL inhibitor resistance using nucleic acids amplified in the above step.
    Type: Application
    Filed: October 16, 2015
    Publication date: September 7, 2017
    Applicants: Toyo Kohan Co., Ltd., Yamaguchi University
    Inventors: Mitsuyoshi OBA, Kenichi ABE, Hirofumi YAMANO, Toshiaki YUJIRI
  • Patent number: 9050577
    Abstract: A solid support for maintaining a uniform spot shape while improving the capacity for immobilization of nucleic acids upon spotting of nucleic acids onto a solid support is provided. A substrate has a base material, an electrostatic layer formed on the base material in order to electrostatically attract nucleic acids, and carboxyl groups formed on the electrostatic layer, wherein the ratio, (COO peak intensity)/(C—C peak intensity) in the C1s spectra obtained by X-ray photoelectron spectrometry (XPS) performed for the surface of the substrate ranges from 0.10 to 0.20. The solid support for immobilizing nucleic acids is produced by active esterification of carboxyl groups of the substrate.
    Type: Grant
    Filed: September 9, 2010
    Date of Patent: June 9, 2015
    Assignee: Toyo Kohan Co., Ltd.
    Inventor: Hirofumi Yamano
  • Patent number: 8778667
    Abstract: The present invention relates to a unit to be used for detection of interactions of biologically relevant molecules using a carrier on which the biologically relevant molecules are immobilized, comprising: a hollow holder having an open part at one end with the other end being closed; and a carrier-supporting member that can be inserted into the hollow holder, on which is mounted a carrier upon which biologically relevant molecules are immobilized, wherein: while the carrier-supporting member is being inserted into the hollow holder, a rear-end portion of the carrier-supporting member is engaged with an edge of the open part of the hollow holder, so that the hollow holder is sealed and the positions of the carrier-supporting member and the hollow holder are determined; and the area on the left and the area on the right of the axial center, which are defined by the inner side of the hollow holder and the external side of the carrier-supporting member on which the carrier has been mounted, are approximately th
    Type: Grant
    Filed: May 26, 2009
    Date of Patent: July 15, 2014
    Assignee: Toyo Kohan Co., Ltd.
    Inventors: Hirofumi Yamano, Shuuichi Kamei, Michifumi Tanga
  • Patent number: 8563239
    Abstract: It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.
    Type: Grant
    Filed: October 6, 2008
    Date of Patent: October 22, 2013
    Assignee: Toyo Kohan Co., Ltd.
    Inventors: Hiroshi Okamura, Michifumi Tanga, Hirofumi Yamano, Mitsuyoshi Ohba
  • Patent number: 8551765
    Abstract: It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.
    Type: Grant
    Filed: July 3, 2003
    Date of Patent: October 8, 2013
    Assignee: Toyo Kohan Co., Ltd.
    Inventors: Hiroshi Okamura, Michifumi Tanga, Hirofumi Yamano, Mitsuyoshi Ohba
  • Patent number: 8497068
    Abstract: A method for detecting hybridization between a probe polynucleotide and a target polynucleotide on a microarray is presented. The method may be used to determine the accuracy of hybridization and possible causes of inaccuracy, such as insufficient washing or deterioration of the microarray.
    Type: Grant
    Filed: June 10, 2010
    Date of Patent: July 30, 2013
    Assignee: Toyo Kohan Co., Ltd.
    Inventors: Koichi Hirayama, Hirofumi Yamano, Michifumi Tanga
  • Patent number: 8481461
    Abstract: Described is a means for objectively determining hybridization failure in addition to performance degradation, insufficient washing and the like in a microarray. In particular a method for detecting the hybridization between a probe polynucleotide and a target polynucleotide by using a microarray is presented.
    Type: Grant
    Filed: December 13, 2010
    Date of Patent: July 9, 2013
    Assignee: Toyo Kohan Co., Ltd.
    Inventors: Hirofumi Yamano, Koichi Hirayama, Akane Ito
  • Publication number: 20120289427
    Abstract: Described is a means for objectively determining hybridization failure in addition to performance degradation, insufficient washing and the like in a microarray. In particular a method for detecting the hybridization between a probe polynucleotide and a target polynucleotide by using a microarray is presented.
    Type: Application
    Filed: December 13, 2010
    Publication date: November 15, 2012
    Applicant: TOYO KOHAN CO., LTD.
    Inventors: Hirofumi Yamano, Koichi Hirayama, Akane Ito
  • Publication number: 20120171503
    Abstract: A solid support for maintaining a uniform spot shape while improving the capacity for immobilization of nucleic acids upon spotting of nucleic acids onto a solid support is provided. A substrate has a base material, an electrostatic layer formed on the base material in order to electrostatically attract nucleic acids, and carboxyl groups formed on the electrostatic layer, wherein the ratio, (COO peak intensity)/(C—C peak intensity) in the C1s spectra obtained by X-ray photoelectron spectrometry (XPS) performed for the surface of the substrate ranges from 0.10 to 0.20. The solid support for immobilizing nucleic acids is produced by active esterification of carboxyl groups of the substrate.
    Type: Application
    Filed: September 9, 2010
    Publication date: July 5, 2012
    Applicant: Toyo Kohan, Co., Ltd.
    Inventor: Hirofumi Yamano
  • Publication number: 20120088690
    Abstract: A method for detecting hybridization between a probe polynucleotide and a target polynucleotide on a microarray is presented. The method may be used to determine the accuracy of hybridization and possible causes of inaccuracy, such as insufficient washing or deterioration of the microarray.
    Type: Application
    Filed: June 10, 2010
    Publication date: April 12, 2012
    Applicant: Toyo Kohan Co., Ltd.
    Inventors: Koichi Hirayama, Hirofumi Yamano, Michifumi Tanga
  • Publication number: 20110111531
    Abstract: The present invention relates to a unit to be used for detection of interactions of biologically relevant molecules using a carrier on which the biologically relevant molecules are immobilized, comprising: a hollow holder having an open part at one end with the other end being closed; and a carrier-supporting member that can be inserted into the hollow holder, on which is mounted a carrier upon which biologically relevant molecules are immobilized, wherein: while the carrier-supporting member is being inserted into the hollow holder, a rear-end portion of the carrier-supporting member is engaged with an edge of the open part of the hollow holder, so that the hollow holder is sealed and the positions of the carrier-supporting member and the hollow holder are determined; and the area on the left and the area on the right of the axial center, which are defined by the inner side of the hollow holder and the external side of the carrier-supporting member on which the carrier has been mounted, are approximately th
    Type: Application
    Filed: May 26, 2009
    Publication date: May 12, 2011
    Applicant: TOYO KOHAN CO., LTD.
    Inventors: Hirofumi Yamano, Shuuichi Kamei, Michifumi Tanga
  • Publication number: 20100300527
    Abstract: [Problem] A substrate for a compound semiconductor solar cell which maintains excellent elasticity even after a high temperature process at the time of forming a thin film is provided. [Means for Resolution] The substrate for a compound semiconductor solar cell is formed of a steel plate, and a Cr layer having a coating film quantity of 300 to 8000 mg/m2 is formed on a surface of the substrate on a side to which a solar cell layer is laminated. The Cr layer having a coating film quantity of 500 to 3000 mg/m2 may be formed on the surface of the substrate on a side to which the solar cell layer is laminated, and a film forming temperature of the solar cell layer is less than 550° C. The Cr layer having a coating film quantity of 2000 to 8000 mg/m2 may be formed on the surface of the substrate on a side to which the solar cell layer is laminated, and a film forming temperature of the solar cell layer is more than 800° C.
    Type: Application
    Filed: May 28, 2010
    Publication date: December 2, 2010
    Applicant: TOYO KOHAN CO., LTD.
    Inventors: Hiroshi Okamura, Hirofumi Yamano, Masanobu Matsubara, Shigeyoshi Nishiyama
  • Publication number: 20090111113
    Abstract: It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.
    Type: Application
    Filed: October 6, 2008
    Publication date: April 30, 2009
    Applicant: Toyo Kohan Co. Ltd.
    Inventors: Hiroshi Okamura, Michifumi Tanga, Hirofumi Yamano, Mitsuyoshi Ohba
  • Publication number: 20090098301
    Abstract: It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.
    Type: Application
    Filed: October 6, 2008
    Publication date: April 16, 2009
    Applicant: Toyo Kohan Co, Ltd.
    Inventors: Hiroshi Okamura, Michifumi Tanga, Hirofumi Yamano, Mitsuyoshi Ohba
  • Publication number: 20060204959
    Abstract: It is intended to provide a solid support capable of immobilizing nucleic acid molecules in a high proportion, and with a high bond strength to nucleic acid molecules. The solid support comprises a substrate and, provided thereon, an electrostatic layer for electrostatically attracting nucleic acid molecules and functional groups capable of covalently binding to nucleic acid molecules.
    Type: Application
    Filed: July 3, 2003
    Publication date: September 14, 2006
    Applicant: Tokyo Kohan Co. Ltd.
    Inventors: Hiroshi Okamura, Michifumi Tanga, Hirofumi Yamano, Mitsuyoshi Ohba
  • Publication number: 20060141465
    Abstract: It is intended to provide a method of immobilizing a polynucleotide on a solid support by spotting the polynucleotide on the solid support in a uniform spot shape without any irregularity. Namely, a method of immobilizing a polynucleotide on a solid support by spotting a solution containing the polynucleotide together with polyether or glycol on the solid support.
    Type: Application
    Filed: November 28, 2003
    Publication date: June 29, 2006
    Applicant: Toyo Kohan Co. Ltd.
    Inventors: Michifumi Tanga, Hiroshi Okamura, Hirofumi Yamano, Mityuyoshi Ohba, Shuuichi Kamei, Teruhisa Ichihara
  • Publication number: 20060121473
    Abstract: Means for effecting rapid mass spectrometry of a multiplicity of samples; and a method of rapidly analyzing biosubstances such as nucleic acids and proteins. The above means is a solid support provided with a carbon layer onto which substances separated by gel electrophoresis are transferred. The above method is one for mass spectrometry of multiple substances which comprises separating substances of a sample by gel electrophoresis, transferring separated substances of the gel onto the above solid support so as to immobilize the same and subjecting the immobilized substances to desorption/ionization.
    Type: Application
    Filed: August 18, 2003
    Publication date: June 8, 2006
    Applicant: Toyo Kohan Co. Ltd.
    Inventors: Michifumi Tanga, Shuuichi Kamei, Hiroshi Okamura, Kaoru Yamakawa, Hirofumi Yamano, Mityuyoshi Ohba, Hisashi Hirano