Patents by Inventor Hirofumi Yoshioka
Hirofumi Yoshioka has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10072250Abstract: The present invention provides a method for efficiently manufacturing a non-enveloped virus with high purity without laborious operation by cultivating cells having the ability to produce a non-enveloped virus and bringing the cells and an acidic solution into contact with each other. A non-enveloped virus vector manufactured by the method of the present invention and a composition having the non-enveloped viral vector as an active ingredient are very useful as gene transfer methods in the fields of basic research and clinical application gene therapy.Type: GrantFiled: July 10, 2014Date of Patent: September 11, 2018Assignee: TAKARA BIO INC.Inventors: Shuhei Sakamoto, Yasuhiro Kawano, Katsuyuki Dodo, Tatsuji Enoki, Hirofumi Yoshioka, Hikaru Takakura, Junichi Mineno
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Patent number: 9399780Abstract: The present invention provides a method for producing a virus vector, which involves a step of culturing a cell capable of producing the virus vector in a culture medium containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate; and a culture medium for use in the production of a virus vector, which is characterized by containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate as active ingredients.Type: GrantFiled: March 14, 2013Date of Patent: July 26, 2016Assignee: TAKARA BIO INC.Inventors: Kazuhisa Shinmura, Hirofumi Yoshioka, Hikaru Takakura, Junichi Mineno
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Publication number: 20160152955Abstract: The present invention provides a method for efficiently manufacturing a non-enveloped virus with high purity without laborious operation by cultivating cells having the ability to produce a non-enveloped virus and bringing the cells and an acidic solution into contact with each other. A non-enveloped virus vector manufactured by the method of the present invention and a composition having the non-enveloped viral vector as an active ingredient are very useful as gene transfer methods in the fields of basic research and clinical application gene therapy.Type: ApplicationFiled: July 10, 2014Publication date: June 2, 2016Applicant: TAKARA BIO INC.Inventors: Shuhei SAKAMOTO, Yasuhiro KAWANO, Katsuyuki DODO, Tatsuji ENOKI, Hirofumi YOSHIOKA, Hikaru TAKAKURA, Junichi MINENO
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Patent number: 9102943Abstract: The present invention provides a method for producing a virus vector, which comprises a step wherein cells that are capable of producing a virus vector are cultured in a culture medium that contains, as active components, a retinoic acid and a histone deacetylase inhibiting substance; and a culture medium for the production of a virus vector, which is characterized by containing, as active components, a retinoic acid and a histone deacetylase inhibiting substance.Type: GrantFiled: October 4, 2011Date of Patent: August 11, 2015Assignee: TAKARA BIO INC.Inventors: Kazuhisa Shinmura, Yoshinori Katayama, Kensuke Sakai, Toshihiro Shodai, Hirofumi Yoshioka, Junichi Mineno
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Patent number: 9051569Abstract: The present invention is intended to provide means of pest control which achieve marked pest control effect. The pest control effect is achieved by the incorporation of an inhibitor (IAP inhibitor) against inhibitor of apoptosis (IAP) into the body of the target pest. The expression of the IAP is preferably inhibited by RNAi.Type: GrantFiled: June 4, 2010Date of Patent: June 9, 2015Assignee: National University Corporation Nagoya UniversityInventors: Teruyuki Niimi, Hirofumi Yoshioka, Yutaka Sato
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Publication number: 20150079050Abstract: The present invention provides a method for producing a virus vector, which involves a step of culturing a cell capable of producing the virus vector in a culture medium containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate; and a culture medium for use in the production of a virus vector, which is characterized by containing a retinoic acid compound, a histone deacetylase-inhibiting substance and a substance capable of forming a chelate as active ingredients.Type: ApplicationFiled: March 14, 2013Publication date: March 19, 2015Inventors: Kazuhisa Shinmura, Hirofumi Yoshioka, Hikaru Takakura, Junichi Mineno
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Publication number: 20130183719Abstract: The present invention provides a method for producing a virus vector, which comprises a step wherein cells that are capable of producing a virus vector are cultured in a culture medium that contains, as active components, a retinoic acid and a histone deacetylase inhibiting substance; and a culture medium for the production of a virus vector, which is characterized by containing, as active components, a retinoic acid and a histone deacetylase inhibiting substance.Type: ApplicationFiled: October 4, 2011Publication date: July 18, 2013Inventors: Kazuhisa Shinmura, Yoshinori Katayama, Kensuke Sakai, Toshihiro Shodai, Hirofumi Yoshioka, Junichi Mineno
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Publication number: 20120151631Abstract: The present invention is intended to provide means of pest control which achieve marked pest control effect. The pest control effect is achieved by the incorporation of an inhibitor (IAP inhibitor) against inhibitor of apoptosis (IAP) into the body of the target pest. The expression of the IAP is preferably inhibited by RNAi.Type: ApplicationFiled: June 4, 2010Publication date: June 14, 2012Applicant: NATIONAL UNIVERSITY CORPORATION NAGOYA UNIVERSITYInventors: Teruyuki Niimi, Hirofumi Yoshioka, Yutaka Sato
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Publication number: 20120142109Abstract: Disclosed is a method for producing a cell population containing memory-like T cells, said method being characterized by including a step for in vitro culturing of a cell population containing T cells or T cell precursor cells using a retinoic acid and a CD3 ligand. Further disclosed is a method for producing a cell population wherein the proportion of memory-like T cells is increased and wherein a desired gene is introduced with a high efficiency and is highly expressed.Type: ApplicationFiled: August 24, 2010Publication date: June 7, 2012Inventors: Yoshinori Katayama, Kensuke Sakai, Hirofumi Yoshioka, Hikaru Takakura, Hideto Chono, Naoki Saito, Junichi Mineno
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Patent number: 7667094Abstract: The present invention relates to isolated promoter sequences responsive to germ infection and functioning in plants; vectors and DNA comprising the promoter sequences are also disclosed. The invention further relates to methods of transforming plants with a DNA construct comprising a germ responsive promoter operably linked to a pathogen resistant gene.Type: GrantFiled: December 1, 2003Date of Patent: February 23, 2010Assignee: Nagoya Industrial Science Research InstituteInventor: Hirofumi Yoshioka
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Patent number: 7485448Abstract: A method for obtaining a virus vector free of a serum, and a serum-free medium for cultivation of a virus producer cell which can be used for the method are provided. By cultivating a virus producer cell using a serum-free medium containing serum albumin, it is possible to cultivate the cell in a state equivalent to that in a serum-containing medium to produce a virus vector at a sufficient titer. The virus vector prepared from the virus producer cell cultivated in the medium exhibits gene transfer efficiency comparable to that of a conventional vector. The medium is useful for gene therapy and studies thereof.Type: GrantFiled: September 13, 2006Date of Patent: February 3, 2009Assignee: Takara Bio Inc.Inventors: Hirofumi Yoshioka, Yasushi Katayama, Daisuke Tomura, Masao Funakoshi, Kazuya Matsumoto, Junichi Mineno, Kazutoh Takesako, Ikunoshin Kato
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Publication number: 20080044903Abstract: Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferring receptor, or introducing a new functional group into the functional substance.Type: ApplicationFiled: July 27, 2007Publication date: February 21, 2008Applicants: Takara Shuzo Co., Ltd., TAKARA BIO INC.Inventors: Mitsuhiro UENO, Hirofumi Yoshioka, Haruko Konishi, Kimikazu Hashino, Mio Morishita, Hideto Chono, Tsuyoshi Miyamura, Mutsumi Sano, Kiyozo Asada, Kei Fujinaga, Ikunoshin Kato
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Publication number: 20070065854Abstract: A method for obtaining a virus vector free of a serum, and a serum-free medium for cultivation of a virus producer cell which can be used for the method are provided. By cultivating a virus producer cell using a serum-free medium containing serum albumin, it is possible to cultivate the cell in a state equivalent to that in a serum-containing medium to produce a virus vector at a sufficient titer. The virus vector prepared from the virus producer cell cultivated in the medium exhibits gene. transfer efficiency comparable to that of a conventional vector. The medium is useful for gene therapy and studies thereof.Type: ApplicationFiled: September 13, 2006Publication date: March 22, 2007Applicant: Takara Bio Inc.Inventors: Hirofumi Yoshioka, Yasushi Katayama, Daisuke Tomura, Masao Funakoshi, Kazuya Matsumoto, Junichi Mineno, Kazutoh Takesako, Ikunoshin Kato
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Publication number: 20060166924Abstract: Gene therapeutics to be used in treating diseases showing sensitivity to gene therapy, characterized by containing as the active ingredient an efficacious amount of a functional substance which has a function of having an affinity for a virus containing a gene usable in the gene therapy and another function of having an affinity specific for a target cell with a need for the gene transfer, or an efficacious amount of a functional substance which has an affinity for the above virus and an efficacious amount of another functional substance which has an affinity specific for the above cell.Type: ApplicationFiled: March 28, 2006Publication date: July 27, 2006Applicant: TAKARA BIO INC.Inventors: Ikunoshin Kato, Kiyozo Asada, Mitsuhiro Ueno, Kimikazu Hashino, Hirofumi Yoshioka, Keiji Tanaka
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Publication number: 20060053509Abstract: It is intended to provide a promoter responding specifically to germ infection (i.e. germ-responsive promoter). Namely, a germ-responsive promoter containing DNA comprising the PVS3 promoter region (SEQ ID NO:1) of potato plant. A region (SEQ ID NO:23) being important in the promoter activity.Type: ApplicationFiled: December 1, 2003Publication date: March 9, 2006Inventor: Hirofumi Yoshioka
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Patent number: 6787359Abstract: Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferrin receptor, or introducing a new functional group into the functional substance.Type: GrantFiled: January 9, 2001Date of Patent: September 7, 2004Assignee: Takara Shuzo Co., Ltd.Inventors: Mitsuhiro Ueno, Hirofumi Yoshioka, Haruko Konishi, Kimikazu Hashino, Mio Morishita, Hideto Chono, Tsuyoshi Miyamura, Mutsumi Sano, Kiyozo Asada, Kei Fujinaga, Ikunoshin Kato
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Publication number: 20040058447Abstract: Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferrin receptor, or introducing a new functional group into the functional substance.Type: ApplicationFiled: September 9, 2003Publication date: March 25, 2004Applicant: Takara Shuzo Co., Ltd.Inventors: Mitsuhiro Ueno, Hirofumi Yoshioka, Haruko Konishi, Kimikazu Hashino, Mio Morishita, Hideto Chono, Tsuyoshi Miyamura, Mutsumi Sano, Kiyozo Asada, Kei Fujinaga, Ikunoshin Kato
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Patent number: 5684146Abstract: An isolated DNA is provided coding for the variable region of an anti-human influenza A type virus antibody having the following characteristics: (a) specifically binds to the stem region of haemaggulutinin molecule of H1N1 and H2N2 subtypes of human influenza A type virus but does not specifically bind to the stem region of haemaggulutinin molecule of H3N2 subtype; and (b) has a neutralization activity for the H1N1 and H2N2 subtypes of human influenza A type virus but no neutralization activity for the H3N2 subtype.Type: GrantFiled: March 10, 1995Date of Patent: November 4, 1997Assignee: Takara Shuzo Co., Ltd.Inventors: Yoshinobu Okuno, Atsushi Oshima, Hirofumi Yoshioka, Takashi Takabatake, Ikunoshin Kato