Patents by Inventor Hirohide Saito

Hirohide Saito has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210079440
    Abstract: The present invention provides an mRNA switch which has improved expression level and sensitivity. An mRNA comprising: (i) a nucleic acid sequence specifically recognized by a miRNA or protein; and (ii) a nucleic acid sequence corresponding to a coding region for a marker protein, wherein a nucleotide contained in the mRNA comprises N1-methyl pseudouridine.
    Type: Application
    Filed: July 12, 2018
    Publication date: March 18, 2021
    Inventors: Hirohide Saito, Kuang YI, Callum PARR
  • Patent number: 10889853
    Abstract: Interaction with a protein is detected by using an RNA probe containing the following sequences; (i) a complementary strand sequence to a DNA barcode sequence, (ii) a sequence of a first stem portion, (iii) a sequence of a second stem portion complementary to the first stem portion for hybridizing with the first stem portion to form a double-stranded stem, and (iv) a sequence of a loop portion contained in RNA for linking the first and second stem portions.
    Type: Grant
    Filed: September 6, 2019
    Date of Patent: January 12, 2021
    Assignee: KYOTO UNIVERSITY
    Inventors: Hirohide Saito, Toshiki Taya, Shunichi Kashida
  • Publication number: 20200232049
    Abstract: A method is provided for distinguishing living cells in a living state with high accuracy. Provided is a method for distinguishing a desired cell type from a cell group comprising two or more types of cells, using the expression of miRNA as an indicator, wherein the method comprises the following steps: (1) a step of introducing mRNA comprising a marker gene operably linked to the target sequence of miRNA used as an indicator into a cell group; and (2) a step of distinguishing a cell type, using the translation level of the marker gene as an indicator.
    Type: Application
    Filed: February 13, 2020
    Publication date: July 23, 2020
    Inventors: HIROHIDE SAITO, KEI ENDO
  • Patent number: 10689624
    Abstract: [Problem to be Solved] To provide a compound for removing pluripotent cells from a cell population potentially containing the pluripotent cells. [Solution] A polyphenylalanine derivative is contacted with a cell population of interest.
    Type: Grant
    Filed: January 14, 2016
    Date of Patent: June 23, 2020
    Assignee: Kyoto University
    Inventors: Hirohide Saito, Yi Kuang
  • Publication number: 20200182923
    Abstract: A testing apparatus for measuring a strength of a chip includes: a cassette mounting base on which to mount a cassette capable of accommodating wafer units; a frame fixing mechanism that fixes an annular frame of the wafer unit; a conveying mechanism that conveys the wafer unit between the cassette and the frame fixing mechanism; a pushing-up mechanism that pushes up a predetermined chip included in the wafer supported by the annular frame fixed by the frame fixing mechanism; a pick-up mechanism having a collet picking up the chip pushed up by the pushing-up mechanism; a strength measuring mechanism having a support unit supporting the chip picked up by the collet; and a collet moving mechanism that moves the collect from a position facing the pushing-up mechanism to a position facing the support unit.
    Type: Application
    Filed: December 9, 2019
    Publication date: June 11, 2020
    Inventors: Makoto KOBAYASHI, Okito UMEHARA, Yoshinobu SAITO, Yusaku ITO, Hirohide YANO, Kazunari TAMURA
  • Patent number: 10620109
    Abstract: The present invention aims to provide a method of sorting a skeletal muscle progenitor cell from a cell population containing the skeletal muscle progenitor cell. The above-mentioned problem is solved by providing a step of introducing miRNA-responsive mRNA into a cell population. The miRNA-responsive mRNA contains (i) a nucleic acid having a sequence specifically recognized by miRNA specifically expressed in a skeletal muscle progenitor cell, and (ii) a nucleic acid containing a sequence encoding a marker protein.
    Type: Grant
    Filed: January 14, 2016
    Date of Patent: April 14, 2020
    Assignee: KYOTO UNIVERSITY
    Inventors: Hirohide Saito, Seiya Takahashi, Hidetoshi Sakurai, Takahiko Sato, Satoru Takayama
  • Patent number: 10604770
    Abstract: A method for extracting differentiated cells from a cell population comprising undifferentiated cells after induction of the differentiation of pluripotent stem cells. A method for extracting differentiated cells from a cell population, comprising the following steps: (1) a step of introducing, into a cell population, mRNA comprising a marker gene operably linked to the target sequence of miRNA specifically expressed in pluripotent stem cells; and (2) a step of extracting cells in which the marker gene has been translated.
    Type: Grant
    Filed: July 16, 2015
    Date of Patent: March 31, 2020
    Assignee: KYOTO UNIVERSITY
    Inventors: Hirohide Saito, Kei Endo, Shota Katayama, Callum Parr
  • Patent number: 10590492
    Abstract: A method for distinguishing living cells in a living state with high accuracy. A method for distinguishing a desired cell type from a cell group comprising two or more types of cells, using the expression of miRNA as an indicator, wherein the method comprises the following steps: (1) a step of introducing mRNA comprising a marker gene operably linked to the target sequence of miRNA used as an indicator into a cell group; and (2) a step of distinguishing a cell type, using the translation level of the marker gene as an indicator.
    Type: Grant
    Filed: January 9, 2015
    Date of Patent: March 17, 2020
    Assignee: KYOTO UNIVERSITY
    Inventors: Hirohide Saito, Kei Endo
  • Publication number: 20200056248
    Abstract: An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.
    Type: Application
    Filed: October 22, 2019
    Publication date: February 20, 2020
    Inventors: Yoshinori Yoshida, Hirohide Saito, Kenji Miki, Kei Endo, Seiya Takahashi
  • Publication number: 20200048685
    Abstract: Interaction with a protein is detected by using an RNA probe containing the following sequences; (i) a complementary strand sequence to a DNA barcode sequence, (ii) a sequence of a first stem portion, (iii) a sequence of a second stem portion complementary to the first stem portion for hybridizing with the first stem portion to form a double-stranded stem, and (iv) a sequence of a loop portion contained in RNA for linking the first and second stem portions.
    Type: Application
    Filed: September 6, 2019
    Publication date: February 13, 2020
    Inventors: Hirohide Saito, Toshiki Taya, Shunichi Kashida
  • Patent number: 10538740
    Abstract: An object of the present invention is to provide a novel method for sorting cardiomyocytes. Another object of the present invention is to provide a method for producing high-purity cardiomyocytes and a kit used therefor. The present invention provides a method for sorting cardiomyocytes, comprising a step of introducing miRNA-responsive mRNA into a cell group, wherein the miRNA-responsive mRNA consists of a sequence comprising the following (i) and (ii): (i) a nucleic acid specifically recognized by miRNA specifically expressed in cardiomyocytes, and (ii) a nucleic acid corresponding to the coding region of a gene, wherein translation of (ii) the nucleic acid corresponding to the coding region of a gene into protein is regulated by the nucleic acid sequence in (i) above, thereby achieving the aforementioned objects.
    Type: Grant
    Filed: March 20, 2015
    Date of Patent: January 21, 2020
    Assignee: Kyoto University
    Inventors: Yoshinori Yoshida, Hirohide Saito, Kenji Miki, Kei Endo, Seiya Takahashi
  • Patent number: 10501811
    Abstract: An object of the present invention is to provide a method for increasing the purity of a type of tissue cell such as an endothelial cell, a hepatocyte, or an insulin-producing cell. The present invention solves the problem by providing a method comprising a step of introducing, into a cell population, an mRNA comprising a nucleic acid sequence recognized by an miRNA specifically expressed in endothelial cells, hepatocytes, or insulin-producing cells.
    Type: Grant
    Filed: April 22, 2016
    Date of Patent: December 10, 2019
    Assignee: KYOTO UNIVERSITY
    Inventors: Yoshinori Yoshida, Hirohide Saito, Kenji Miki, Kei Endo, Seiya Takahashi
  • Patent number: 10435738
    Abstract: Interaction with a protein is detected by using an RNA probe containing the following sequences; (i) a complementary strand sequence to a DNA barcode sequence, (ii) a sequence of a first stem portion, (iii) a sequence of a second stem portion complementary to the first stem portion for hybridizing with the first stem portion to form a double-stranded stem, and (iv) a sequence of a loop portion contained in RNA for linking the first and second stem portions.
    Type: Grant
    Filed: January 9, 2015
    Date of Patent: October 8, 2019
    Assignee: KYOTO UNIVERSITY
    Inventors: Hirohide Saito, Toshiki Taya, Shunichi Kashida
  • Publication number: 20190256866
    Abstract: An mRNA forcibly expresses a protein gene in response to a miRNA, and a method for forcibly expressing the same, are provided. An artificial mRNA comprising a sequence encoding a protein gene, a miRNA target sequence linked to the 3?-terminal side of a Poly A sequence, and a translational repression sequence linked to the 3?-terminal side of the miRNA target sequence; and a method for expressing a protein gene in response to the expression of a miRNA, comprising a step of introducing the artificial mRNA into a cell.
    Type: Application
    Filed: June 27, 2017
    Publication date: August 22, 2019
    Applicant: KYOTO UNIVERSITY
    Inventors: Hirohide Saito, Yoshihiko Fujita
  • Publication number: 20190256830
    Abstract: A method is provided for regulating the activity of a nuclease in response to a cell-specific miRNA by using a nucleic acid sequence specifically recognized by the miRNA in conjunction with a nucleic acid sequence encoding the nuclease.
    Type: Application
    Filed: May 18, 2017
    Publication date: August 22, 2019
    Applicant: KYOTO UNIVERSITY
    Inventors: Hirohide Saito, Moe Hirosawa
  • Patent number: 10378070
    Abstract: Provided is a method for continuously visualizing and accurately determining a living cellular state per se. Disclosed are: a vector comprising 5?- and 3?-end nucleic acid sequences for integration and a reporter gene sequence positioned between the 5?- and 3?-end nucleic acid sequences for integration; a gene introduction agent comprising a first vector and a second vector; a cell comprising the vector or the agent; and a method for determining a cellular state by using the cell.
    Type: Grant
    Filed: September 7, 2017
    Date of Patent: August 13, 2019
    Assignee: Kyoto University
    Inventors: Hirohide Saito, Hideyuki Nakanishi
  • Publication number: 20190151474
    Abstract: Engineered synthetic RNA-based genetic circuits are provided that are regulated exclusively at the post-transcriptional level.
    Type: Application
    Filed: September 8, 2015
    Publication date: May 23, 2019
    Applicants: Massachusetts Institute of Technology, Kyoto University
    Inventors: Ron Weiss, Liliana Wroblewska, Velia Siciliano, Tasuku Kitada, Maria Hottelet Foley, Katie Bodner, Hirohide Saito, Kei Endo, Darrell J. Irvine, Tyler Wagner, Jacob Becraft
  • Publication number: 20190144873
    Abstract: A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5?UTR regulation structure comprising: (1) a cap structure at the 5? terminus, (2) a spacer positioned on the 3? side of the cap structure, and (3) one or more RNA motifs positioned on the 3? side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3? side of the 5?UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases.
    Type: Application
    Filed: January 25, 2019
    Publication date: May 16, 2019
    Applicant: Kyoto University
    Inventors: Hirohide Saito, Kei Endo, Tan Inoue
  • Patent number: 10227596
    Abstract: A translational control method using an RNA-protein interaction motif is provided. The method comprises a step of introducing an mRNA having: a 5?UTR regulation structure comprising: (1) a cap structure at the 5? terminus, (2) a spacer positioned on the 3? side of the cap structure, and (3) one or more RNA motifs positioned on the 3? side of the spacer, which comprises an RNA-protein interaction motif-derived nucleotide sequence or a variant thereof; and a nucleotide sequence encoding a target protein gene on the 3? side of the 5?UTR regulation structure, into a cell in the presence of a protein specifically binding to the RNA motifs, wherein a translational level is decreased as the number of bases of the spacer decreases, and the translational level is decreased as the number of the RNA motifs increases.
    Type: Grant
    Filed: August 24, 2016
    Date of Patent: March 12, 2019
    Assignee: Kyoto University
    Inventors: Hirohide Saito, Kei Endo, Tan Inoue
  • Publication number: 20190071736
    Abstract: Provided is a method for continuously visualizing and accurately determining a living cellular state per se. Disclosed are: a vector comprising 5?- and 3?-end nucleic acid sequences for integration and a reporter gene sequence positioned between the 5?- and 3?-end nucleic acid sequences for integration; a gene introduction agent comprising a first vector and a second vector; a cell comprising the vector or the agent; and a method for determining a cellular state by using the cell.
    Type: Application
    Filed: September 7, 2017
    Publication date: March 7, 2019
    Inventors: Hirohide Saito, Hideyuki Nakanishi