Abstract: An object achieved by the present invention is to provide a coated article having excellent appearance, corrosion resistance, weatherability, and chipping resistance obtained by a wet-on-wet coating.

Abstract: The present invention provides the following method for forming a multilayer coating film. The method comprises forming an uncured coating film of a specific primer paint composition (A) on a substrate, forming a top coating film of a specific top paint composition (B) on the uncured coating film, and simultaneously drying the films. The primer paint composition (A) is a paint composition comprising an epoxy resin (a1), a rust preventive pigment (a2), a color pigment (a3), and an extender pigment (a4); and the top paint composition (B) is a paint composition comprising an acrylic resin (b1) and an active methylene blocked polyisocyanate compound (b2).

Abstract: The present invention provides the following method for forming a multilayer coating film. The method comprises forming an uncured coating film of a specific primer paint composition (A) on a substrate, forming a top coating film of a specific top paint composition (B) on the uncured coating film, and simultaneously drying the films. The primer paint composition (A) is a paint composition comprising an epoxy resin (a1), a rust preventive pigment (a2), a color pigment (a3), and an extender pigment (a4); and the top paint composition (B) is a paint composition comprising an acrylic resin (b1) and an active methylene blocked polyisocyanate compound (b2).

Abstract: An object achieved by the present invention is to provide a coated article having excellent appearance, corrosion resistance, weatherability, and chipping resistance obtained by a wet-on-wet coating.

Abstract: The present invention provides a thermosetting powder coating composition that is capable of forming a coating film having excellent moisture resistance, excellent corrosion resistance, etc. More specifically, the present invention provides a thermosetting powder coating composition comprising a carboxy-containing resin (A), a ?-hydroxyalkylamide curing agent (B), and an anticorrosive (C) that comprises a strontium carbonate-modified aluminum phosphate.

Abstract: The method of the invention provides the use of a replacement-type targeting construct to delete large fragments of genomic DNA by gene targeting. The replacement targeting construct, which may contain a selectable marker, is constructed to contain two regions of sequences which are homologous to the 5? and 3? flanking sequences of the targeted locus. After transfection of the targeting construct into the desired cell line, gene targeted-mediated deletions are identified by selection and further characterized. The invention is useful in any situation where one would want to create a large genomic deletion. Examples of suitable loci include MHC Class I and II antigens and immunoglobulin genes, including, for example, variable and constant region of kappa, lambda, or heavy chains.

Abstract: The method of the invention provides the use of a replacement-type targeting construct to delete large fragments of genomic DNA by gene targeting. The replacement targeting construct, which may contain a selectable marker, is constructed to contain two regions of sequences which are homologous to the 5' and 3' flanking sequences of the targeted locus. After transfection of the targeting construct into the desired cell line, gene targeted-mediated deletions are identified by selection and further characterized. The invention is useful in any situation where one would want to create a large genomic deletion. Examples of suitable loci include MHC Class I and II antigens and immunoglobulin genes, including, for example, variable and constant region of kappa, lambda, or heavy chains.