Abstract: Provided are a uricase activator and a uricase activation method which are capable of highly activating uricase. Also provided are a uric acid measurement reagent and a uric acid measurement method which have a wide measurable concentration range. Further provided are a uricase activator comprising hydroxyisourate hydrolase, and a uric acid measurement reagent for use in measuring a uric acid concentration in a sample collected from a living body, comprising uricase and hydroxyisourate.

Abstract: A blade attachment tool includes a first support member supported by a pressing support portion supporting a blade formed of an elastic member configured to wipe/scrape liquid/solids spreading on an abutment surface by being pressed against the abutment surface and being long in one direction such that the blade is pressed against the abutment surface with a predetermined pressing force, a second support member supported to swing about a swing shaft extending in a longitudinal direction of the blade with respect to the first support member and configured such that a base end side of the blade is attached thereto, and an energizing member configured to apply a reaction force to a friction force that causes the second support member to swing about the swing shaft with respect to the first support member when the blade is pressed against the abutment surface to slide relatively to the abutment surface.

Abstract: A compound includes ?-nicotinamide mononucleotide or a pharmacologically acceptable salt thereof. A purity of the compound as measured through HPLC is 95% or higher. A reactivity of the compound with lactate dehydrogenase is 30 units or higher.

Abstract: A blade attachment tool includes a first support member supported by a pressing support portion supporting a blade formed of an elastic member configured to wipe/scrape liquid/solids spreading on an abutment surface by being pressed against the abutment surface and being long in one direction such that the blade is pressed against the abutment surface with a predetermined pressing force, a second support member supported to swing about a swing shaft extending in a longitudinal direction of the blade with respect to the first support member and configured such that a base end side of the blade is attached thereto, and an energizing member configured to apply a reaction force to a friction force that causes the second support member to swing about the swing shaft with respect to the first support member when the blade is pressed against the abutment surface to slide relatively to the abutment surface.

Abstract: An inner peripheral surface of a belt is cogged so that cogs extend in a belt width direction and are arranged adjacent to one another at regular intervals in a belt length direction. A tip portion of the cog and a bottom portion of a groove formed between the cogs have curved surfaces each having an arc-shaped cross section. A curvature radius of a cross-sectional shape of the bottom portion of the groove is set to be larger than a curvature radius of a cross-sectional shape of the tip portion of the cog.

Abstract: An inner peripheral surface of a belt is cogged so that cogs extend in a belt width direction and are arranged adjacent to one another at regular intervals in a belt length direction. A tip portion of the cog and a bottom portion of a groove formed between the cogs have curved surfaces each having an arc-shaped cross section. A curvature radius of a cross-sectional shape of the bottom portion of the groove is set to be larger than a curvature radius of a cross-sectional shape of the tip portion of the cog.

Abstract: The present invention provides a novel process for preparing nicotinamide adenine dinucleotide phosphate (NADP). The process of the present invention comprises performing phosphorylation using a polyphosphoric acid or a salt thereof and nicotinamide adenine dinucleotide (NAD+) as substrates in the presence of a polyphosphate-dependent NAD+ kinase from a Mycobacterium, wherein the reaction solution contains 0.1-15% by weight of the polyphosphoric acid or a salt thereof, and 5-150 mM of a divalent metal ion.

Abstract: Provided is a V-ribbed belt that can suppress generation of slippage when it is splashed with water, and generation of noise. A V-ribbed belt has a rib-forming surface with plural rows of ribs formed therein and extending in a belt longitudinal direction to allow the rib-forming surface to be brought into contact with pulleys when in use, wherein the V-ribbed belt has reentrant portions on the rib-forming surface to form a non-contact area that is held out of contact with the pulleys when the V-ribbed belt is driven, so that water is allowed to flow into the non-contact area when the V-ribbed belt is splashed with water.

Abstract: The present invention relates to an enzyme immunoassay method for assaying calcium ion, including putting calcium-dependent glutamate dehydrogenase, namely glutamate dehydrogenase (GLDH) never expressing its activity in the absence of calcium ion, in contact to the calcium ion in a sample to assay the enzyme activity of the GLDH, the activity changing depending on the calcium ion concentration in the sample; and the invention also relates to an assay reagent for use in the same. Because the reagent is very stable in a solution state under storage, the reagent can be incorporated in a liquid reagent. Thus, the calcium in a biological material or other samples can be assayed in a simple and accurate manner.

Abstract: A recombinant human creatine kinase heterodimer with solution-stability is disclosed. The CK heterodimer is characterized by being produced by using a vector which contains a nucleic acid encoding the M type subunit of creatine kinase and a nucleic acid encoding the B type subunit thereof.

Abstract: A recombinant human creatine kinase heterodimer with solution-stability is disclosed. The CK heterodimer is characterized by being produced by using a vector which contains a nucleic acid encoding the M type subunit of creatine kinase and a nucleic acid encoding the B type subunit thereof.

Abstract: The present invention relates to an enzyme immunoassay method for assaying calcium ion, including putting calcium-dependent glutamate dehydrogenase, namely glutamate dehydrogenase (GLDH) never expressing its activity in the absence of calcium ion, in contact to the calcium ion in a sample to assay the enzyme activity of the GLDH, the activity changing depending on the calcium ion concentration in the sample; and the invention also relates to an assay reagent for use in the same. Because the reagent is very stable in a solution state under storage, the reagent can be incorporated in a liquid reagent. Thus, the calcium in a biological material or other samples can be assayed in a simple and accurate manner.

Abstract: The invention provides an ammonia elimination reagent in solution, comprising a thermo-resistant isocitrate dehydrogenase with prominent stability under conditions at high alkaline pHs. For example, the isocitrate dehydrogenase is preferably derived from the genus Thermus. For an assay system of biological substances generating a reaction product ammonia, an ammonia elimination reagent can be prepared by selecting and using the enzyme; the resulting ammonia elimination reagent can be stored in solution; additionally, the assay system can be designed in combination with both the coenzymes NAD+ and NADP+. The ammonia elimination reagent is novel and can eliminate ammonia in an extremely short time.

Abstract: The present invention provides a process for mass producing recombinant, thermostable GLDH at a low cost. The invention also provides a process for mass producing recombinant, thermostable GLDH at a low cost, by culturing a transformant without using a drug such as IPTG. The invention further provides thermostable GLDH which can use more stable NADH as a coenzyme in an ammonia assay reagent.

Abstract: The present invention provides a gene encoding the type B subunit protein of avian lactate dehydrogenase. The gene encoding the type B subunit protein of avian lactate dehydrogenase of the present invention is obtained by plaque hybridization or the like from a cDNA library derived from avian heart muscle and encodes the type B subunit protein of avian lactate dehydrogenase having an amino acid sequence shown as SEQ ID NO. 2.

Abstract: The present invention relates to a hexokinase that is stable in solution whether in the presence or the absence of glucose. The present enzyme is obtained from a culture of the thermophilic yeast, Kluyveromyces. It can be used for determination of ATP and glucose, and for determination of a biological component which produces ATP or glucose (e.g. creatine kinase).

Abstract: The invention relates to an ammonia elimination reagent for use in an enzymatic assay of a biological substance which produces ammonia as the reaction product. The reagent is an alkaline solution of pH 9 to 11 and contains Sulfolobus-derived thermostable isocitrate dehydrogenase, 2-oxoglutaric acid, a reducing coenzyme, isocitric acid, glutamate dehydrogenase, and a divalent metal salt. The reagent can be stored in solution and has excellent stability under conditions of alkaline pHs.

Abstract: The present invention provides a gene encoding the type B subunit protein of avian lactate dehydrogenase. The gene encoding the type B subunit protein of avian lactate dehydrogenase of the present invention is obtained by plaque hybridization or the like from a cDNA library derived from avian heart muscle and encodes the type B subunit protein of avian lactate dehydrogenase having the sequences shown as SEQ ID NO. 1 or 2.

Abstract: GMT is produced by E. coli transformant with an expression vector having introduced therein cDNA of glycine-N-methyl transferase (GMT) derived from rat liver. This process can produce GMT of a high purity and a high activity on an industrial scale.