Patents by Inventor Hirokazu Sanada
Hirokazu Sanada has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11725193Abstract: [Problem] To provide a glucose dehydrogenase with high thermal stability to be used in blood sugar measurement. [Solution to Problem to problem] A modified glucose dehydrogenase with an excellent property of high thermal stability that can be obtained by modifying some of amino acids constituting a protein such as a wild-type glucose dehydrogenase, a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition and a biosensor, and methods for manufacturing the measuring reagent composition and the biosensor.Type: GrantFiled: May 25, 2018Date of Patent: August 15, 2023Assignee: IKEDA FOOD RESEARCH CO., LTD.Inventors: Michinari Honda, Hirokazu Sanada, Ryo Takenaka, Takafumi Takumi, Takahiro Fujii
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Patent number: 11345897Abstract: The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level.Type: GrantFiled: February 15, 2019Date of Patent: May 31, 2022Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Tetsunari Morita, Mika Kuyama, Tokuji Ikeda, Kenji Kano, Seiya Tsujimura
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Publication number: 20220098557Abstract: [Problem] To provide a glucose dehydrogenase with high thermal stability to be used in blood sugar measurement. [Solution to Problem to problem] A modified glucose dehydrogenase with an excellent property of high thermal stability that can be obtained by modifying some of amino acids constituting a protein such as a wild-type glucose dehydrogenase, a polynucleotide encoding the enzyme, a method for manufacturing the enzyme, a method for measuring glucose using the enzyme, a measuring reagent composition and a biosensor, and methods for manufacturing the measuring reagent composition and the biosensor.Type: ApplicationFiled: May 25, 2018Publication date: March 31, 2022Applicant: IKEDA FOOD RESEARCH CO., LTD.Inventors: Michinari HONDA, Hirokazu SANADA, Ryo TAKENAKA, Takafumi TAKUMI, Takahiro FUJII
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Patent number: 11225645Abstract: The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase, According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level.Type: GrantFiled: February 15, 2019Date of Patent: January 18, 2022Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Tetsunari Morita, Mika Kuyama, Tokuji Ikeda, Kenji Kano, Seiya Tsujimura
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Patent number: 11155789Abstract: The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level.Type: GrantFiled: February 15, 2019Date of Patent: October 26, 2021Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Tetsunari Morita, Mika Kuyama, Tokuji Ikeda, Kenji Kano, Seiya Tsujimura
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Publication number: 20210238562Abstract: The present invention addresses the problem of providing: a glucose dehydrogenase; a polynucleotide encoding the enzyme; a method for producing the enzyme; a method for measuring glucose using the enzyme; a measurement reagent composition; and a biosensor. The present invention pertains to a protein that has any of amino acid sequences (a), (b) and (c) and has a glucose dehydrogenase activity, etc.: (a) an amino acid sequence represented by SEQ ID NO: 3, 6, 15 or 16; (b) an amino acid sequence derived from the amino acid sequence represented by SEQ ID NO: 3, 6, 15 or 16 by deleting, substituting or adding 1-3 amino acids; and (c) an amino acid sequence having 90% or more identity to the amino acid sequence represented by SEQ ID NO: 3 or 15 or 85% or more identity to the amino acid sequence represented by SEQ ID NO: 6 or 16.Type: ApplicationFiled: April 15, 2019Publication date: August 5, 2021Applicant: IKEDA FOOD RESEARCH CO., LTD.Inventors: Takafumi TAKUMI, Emi WATANABE, Ryo TAKENAKA, Hirokazu SANADA, Michinari HONDA
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Patent number: 10988738Abstract: The present invention provides a microorganism-derived soluble coenzyme-binding glucose dehydrogenase which catalyzes a reaction for oxidizing glucose in the presence of an electron acceptor, has an activity to maltose as low as 5% or less, and is inhibited by 1,10-phenanthroline. The invention also provides a method for producing the coenzyme-binding glucose dehydrogenase, and a method and a reagent for measuring employing the coenzyme-binding glucose dehydrogenase. According to the invention, the coenzyme-binding glucose dehydrogenase can be applied to an industrial field, and a use becomes possible also in a material production or analysis including a method for measuring or eliminating glucose in a sample using the coenzyme-binding glucose dehydrogenase as well as a method for producing an organic compound. It became also possible to provide a glucose sensor capable of accurately measuring a blood sugar level.Type: GrantFiled: February 15, 2019Date of Patent: April 27, 2021Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Tetsunari Morita, Mika Kuyama, Tokuji Ikeda, Kenji Kano, Seiya Tsujimura
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Patent number: 10883133Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: GrantFiled: September 28, 2018Date of Patent: January 5, 2021Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
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Patent number: 10851398Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: GrantFiled: September 28, 2018Date of Patent: December 1, 2020Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
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Patent number: 10815515Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: GrantFiled: September 28, 2018Date of Patent: October 27, 2020Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
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Patent number: 10808274Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: GrantFiled: March 21, 2018Date of Patent: October 20, 2020Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
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Patent number: 10738341Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: GrantFiled: March 21, 2018Date of Patent: August 11, 2020Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
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Patent number: 10669565Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: GrantFiled: September 28, 2018Date of Patent: June 2, 2020Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
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Patent number: 10648011Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: GrantFiled: March 21, 2018Date of Patent: May 12, 2020Assignees: Ikeda Food Research Co., ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
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Patent number: 10626434Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: GrantFiled: September 28, 2018Date of Patent: April 21, 2020Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
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Patent number: 10626433Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: GrantFiled: March 21, 2018Date of Patent: April 21, 2020Assignees: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori Omura, Hirokazu Sanada, Takako Yada, Ayaka Atsumi, Tetsunari Morita, Emi Ishimaru
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Publication number: 20190177762Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: ApplicationFiled: March 21, 2018Publication date: June 13, 2019Applicants: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori OMURA, Hirokazu SANADA, Takako YADA, Ayaka ATSUMI, Tetsunari MORITA, Emi ISHIMARU
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Publication number: 20190177761Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: ApplicationFiled: March 21, 2018Publication date: June 13, 2019Applicants: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori OMURA, Hirokazu SANADA, Takako YADA, Ayaka ATSUMI, Tetsunari MORITA, Emi ISHIMARU
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Publication number: 20190177763Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: ApplicationFiled: March 21, 2018Publication date: June 13, 2019Applicants: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori OMURA, Hirokazu SANADA, Takako YADA, Ayaka ATSUMI, Tetsunari MORITA, Emi ISHIMARU
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Publication number: 20190062798Abstract: The present invention provides members that produce on a large scale a coenzyme-linked glucose dehydrogenase which has excellent substrate-recognizing ability toward glucose while providing low action on maltose. The present invention relates to a polynucleotide encoding a soluble coenzyme-linked glucose dehydrogenase that catalyzes the oxidation of glucose in the presence of an electron acceptor and has an activity toward maltose of 5% or lower; a polypeptide encoded by the nucleotide sequence of the polynucleotide; a recombinant vector carrying the polynucleotide; a transformed cell produced using the recombinant vector; a method for producing a polypeptide comprising culturing the transformed cell and collecting from the cultivated products a polypeptide that links to FAD to exert the glucose dehydration activity; a method for determination of glucose using the polypeptide; a reagent composition for determination of glucose; and a biosensor.Type: ApplicationFiled: September 28, 2018Publication date: February 28, 2019Applicants: Ikeda Food Research Co., Ltd., PHC CorporationInventors: Hironori OMURA, Hirokazu SANADA, Takako YADA, Ayaka ATSUMI, Tetsunari MORITA, Emi ISHIMARU