Abstract: To detect a biological substance at high speed and high sensitivity by using a well array and particles. The biological substance detection method of the present invention includes a step of preparing a test liquid containing particles at a concentration of at least 5×107 particles/mL, a step of allowing the particles to trap the biological substance to form a trapped body, a step of sending the test liquid onto the well array to store a plurality of the particles including the trapped body in the wells while storing the number of the particles to be stored in one well to a minimum average storage number Nmin represented by the following formulas (1) and (2), and a step of detecting the color development of the well with an image pickup element.

Abstract: An object is to detect a virus with high speed and high sensitivity. Provided is a virus detection method including using a well array formed by defining a plurality of wells adjacent to each other by a side wall stood on a substrate and detecting a virus in a test liquid based on the detection of light emission from the wells, wherein the well array has a test region in which a well formation area, which is an area of the substrate in a region where the wells are formed, is 5 mm2 or more, a total volume of the wells obtained by integrating individual volumes of the wells is 17 nL or more, a depth of the wells is 3.5 ?m to 40 ?m, an opening area of the wells is 18 ?m2 to 1,700 ?m2, a volume of the wells is 74 fL to 6,000 fL, and a thickness of the side wall between the wells adjacent to each other is 0.5 ?m to 15 ?m; and the detection of light emission from the wells is performed in a detection unit in which an observation visual field is fixed to have a size including the test region.

Abstract: Provided is a target substance detection device that can detect a target substance accurately and efficiently and can be manufactured at low costs.

Abstract: To detect a target substance accurately and effectively, a target substance detection device 1 includes: a liquid-sample introducing plate 2 formed from a light-transmissive plate having a surface on which a liquid sample including a target substance and magnetic particles forming a conjugate with the target substance is introduced, and enabling propagating transmitted light of light irradiated from the rear face upward of the surface as propagated light; a rear face light irradiation unit 3 configured to be able to irradiate the liquid-sample introducing plate 2 with light from the rear face; a first magnetic field application unit 4 disposed on the side of the surface of the liquid-sample introducing plate 2, and configured to apply a magnetic field to move the conjugate in the liquid sample that is introduced on the surface of the liquid-sample introducing plate in the direction away from the liquid-sample introducing plate 2; and an optical-signal detection unit 5 disposed on the side of the surface of th

Abstract: A target substance detection device detecting a target substance using magnetic particles includes a detection chip, a light irradiation unit, and a magnetic field application unit, wherein the detection chip includes a light transmissive member having a sensing surface arranged on a surface constituting a top surface relative to a bottom surface, an inclined surface, and a main body portion capable of receiving light and guiding the light through the interior, the light transmissive member having a light directing structure in which the light applied from the top surface side and passed through the inclined surface is directed via the main body portion to the sensing surface under the condition of total reflection, the light irradiation unit is operable to irradiate the sensing surface with light under the condition of total reflection via the light directing structure, and the magnetic field application unit.

Abstract: The invention relates to a method that detects the target substance by measuring a decrease in the optical signal generated by applying a first magnetic field to move the conjugate in the direction parallel to or away from the surface, etc. The invention also relates to an optical detection device wherein a liquid holding part has a sensing plate that can form a near field on a surface with light irradiated from a side of a rear face under a condition of total reflection, the liquid holding part being capable of holding a liquid sample that has a target substance and a magnetic particle that forms a conjugate with the target substance on the surface of the sensing plate, a light irradiation unit, a light detection unit, and a magnetic field application unit configured to apply a magnetic field to move the conjugate in the direction parallel to or away from the surface, etc.

Abstract: Provided is, among others, a target substance detection device that can be used in detecting a target substance using magnetic particles and that can be manufactured compactly and inexpensively.

Abstract: To detect a target substance accurately and effectively, a target substance detection device 1 includes: a liquid-sample introducing plate 2 formed from a light-transmissive plate having a surface on which a liquid sample including a target substance and magnetic particles forming a conjugate with the target substance is introduced, and enabling propagating transmitted light of light irradiated from the rear face upward of the surface as propagated light; a rear face light irradiation unit 3 configured to be able to irradiate the liquid-sample introducing plate 2 with light from the rear face; a first magnetic field application unit 4 disposed on the side of the surface of the liquid-sample introducing plate 2, and configured to apply a magnetic field to move the conjugate in the liquid sample that is introduced on the surface of the liquid-sample introducing plate in the direction away from the liquid-sample introducing plate 2; and an optical-signal detection unit 5 disposed on the side of the surface of th

Abstract: A problem is to provide an optical detection method that enables sensitive and quick detection of a micro target substance in a near field and such a device. As means for solving the problem, an optical detection method for a target substance detects light, such as fluorescence, as an optical signal from a conjugate comprising the target substance on a surface of a sensing plate in a near field, wherein the conjugate comprises at least the target substance bonding with a magnetic particle. The method comprises: detecting the target substance by measuring a decrease in the optical signal generated by applying a first magnetic field to move the conjugate in the direction parallel to or away from the surface, etc.