Patents by Inventor Hiroki Higashibata

Hiroki Higashibata has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Inducible high expression system
    Patent number: 7718396
    Abstract: The present invention provides a vector containing a DNA construct containing a first promoter that is a nitrilase gene promoter, a multicloning site and a nitrilase transcription regulatory protein gene (particularly preferably a structural gene is ligated into a multicloning site and a second promoter is placed at the upstream of the nitrilase transcription regulatory protein gene), and the like. The present invention affords a high expression system capable of expressing a large amount of useful proteins by actinomycete host represented by actinomycete belonging to the genus Streptomyces and the like beneficially used for the production of useful proteins.
    Type: Grant
    Filed: March 4, 2003
    Date of Patent: May 18, 2010
    Assignees: Astellas Pharma Inc.
    Inventors: Michihiko Kobayashi, Yoshiteru Hashimoto, Hiroki Higashibata
  • DNA helicase
    Patent number: 6939699
    Abstract: The present invention provides a heat-stable enzyme having a DNA helicase activity, and preferably a structure-specific endonuclease activity. This enzyme can be obtained by cloning a gene encoding the enzyme from chromosomal DNA of a hyperthermophile, preferably a sulfur-metabolizing thermophilic archaebacteria, incorporating the gene into an expression vector, introducing the vector into a host such as Escherichia coli in the usual manner to create a transformant, and culturing the transformant.
    Type: Grant
    Filed: February 26, 2003
    Date of Patent: September 6, 2005
    Assignee: National Institute of Advanced Industrial Science and Technology
    Inventors: Ikuo Matsui, Hiroki Higashibata, Yutaka Kawarabayashi
  • Inducible high expression system
    Publication number: 20040053273
    Abstract: The present invention provides a vector containing a DNA construct containing a first promoter that is a nitrilase gene promoter, a multicloning site and a nitrilase transcription regulatory protein gene (particularly preferably a structural gene is ligated into a multicloning site and a second promoter is placed at the upstream of the nitrilase transcription regulatory protein gene), and the like. The present invention affords a high expression system capable of expressing a large amount of useful proteins by actinomycete host represented by actinomycete belonging to the genus Streptomyces and the like beneficially used for the production of useful proteins.
    Type: Application
    Filed: March 4, 2003
    Publication date: March 18, 2004
    Applicants: Michihiko KOBAYASHI, Fujisawa Pharmaceutical Co., Ltd.
    Inventors: Michihiko Kobayashi, Yoshiteru Hashimoto, Hiroki Higashibata
  • DNA helicase
    Publication number: 20030232357
    Abstract: The present invention provides a heat-stable enzyme having a DNA helicase activity, and preferably a structure-specific endonuclease activity. This enzyme can be obtained by cloning a gene encoding the enzyme from chromosomal DNA of a hyperthermophile, preferably a sulfur-metabolizing thermophilic archaebacteria, incorporating the gene into an expression vector, introducing the vector into a host such as Escherichia coli in the usual manner to create a transformant, and culturing the transformant.
    Type: Application
    Filed: February 26, 2003
    Publication date: December 18, 2003
    Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
    Inventors: Ikuo Matsui, Hiroki Higashibata, Yutaka Kawarabayashi