Patents by Inventor Hiroki Nakae

Hiroki Nakae has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11566273
    Abstract: Provided is a method for producing a cell contained base, the method being capable of providing a cell contained base highly accurately controlled in number of nucleic acid molecules contained in a low-concentration nucleic acid standard sample, the method including a liquid droplet discharging step of discharging a cell suspension in the form of a liquid droplet with a liquid droplet discharging unit onto a base including at least one cell contained region; a cell number counting step of counting a number of cells contained in the liquid droplet with a plurality of sensors from two or more directions while the liquid droplet is flying into the cell contained region; and a liquid droplet landing step of landing the liquid droplet in the at least one cell contained region in a manner that a predetermined number of cells are located in the at least one cell contained region.
    Type: Grant
    Filed: June 13, 2018
    Date of Patent: January 31, 2023
    Assignee: RICOH COMPANY, LTD.
    Inventors: Satoshi Izumi, Manabu Seo, Takahiko Matsumoto, Hiroki Somada, Ryuya Mashiko, Takeshi Orito, Hiroki Nakae
  • Publication number: 20180363028
    Abstract: Provided is a method for producing a cell contained base, the method being capable of providing a cell contained base highly accurately controlled in number of nucleic acid molecules contained in a low-concentration nucleic acid standard sample, the method including a liquid droplet discharging step of discharging a cell suspension in the form of a liquid droplet with a liquid droplet discharging unit onto a base including at least one cell contained region; a cell number counting step of counting a number of cells contained in the liquid droplet with a plurality of sensors from two or more directions while the liquid droplet is flying into the cell contained region; and a liquid droplet landing step of landing the liquid droplet in the at least one cell contained region in a manner that a predetermined number of cells are located in the at least one cell contained region.
    Type: Application
    Filed: June 13, 2018
    Publication date: December 20, 2018
    Inventors: Satoshi IZUMI, Manabu SEO, Takahiko MATSUMOTO, Hiroki SOMADA, Ryuya MASHIKO, Takeshi ORITO, Hiroki NAKAE
  • Patent number: 8530231
    Abstract: A vacuum blood collection tube (100) comprises a bottomed tube (101) composed of a low temperature resistant material that is less susceptible to low temperature fracture when cryopreserved at ultra-low temperature, a stopper (102) having a needle piercing portion (113) composed of a rubber material that can be pierced with a blood collection needle (110), and a cryopreservation cap (103) composed of the low temperature resistant material. Before blood collection, the stopper (102) is attached to the bottomed tube (101) to maintain the reduced pressure state inside the bottomed tube (101). Thus, blood collection by a vacuum blood collection method is possible. After blood collection, the cryopreservation cap (103) is attached to the bottomed tube (101), so that the liquid tight state of the bottomed tube (101) is maintained, and cryopreservation at ultra-low temperature as it is possible.
    Type: Grant
    Filed: December 27, 2007
    Date of Patent: September 10, 2013
    Assignee: Kaneka Corporation
    Inventors: Hiroki Nakae, Haruka Uesaka, Atsuhiko Minekawa, Takeshi Kurono
  • Publication number: 20100323437
    Abstract: A vacuum blood collection tube (100) comprises a bottomed tube (101) composed of a low temperature resistant material that is less susceptible to low temperature fracture when cryopreserved at ultra-low temperature, a stopper (102) having a needle piercing portion (113) composed of a rubber material that can be pierced with a blood collection needle (110), and a cryopreservation cap (103) composed of the low temperature resistant material. Before blood collection, the stopper (102) is attached to the bottomed tube (101) to maintain the reduced pressure state inside the bottomed tube (101). Thus, blood collection by a vacuum blood collection method is possible. After blood collection, the cryopreservation cap (103) is attached to the bottomed tube (101), so that the liquid tight state of the bottomed tube (101) is maintained, and cryopreservation at ultra-low temperature as it is possible.
    Type: Application
    Filed: December 27, 2007
    Publication date: December 23, 2010
    Applicant: MEDIBIC
    Inventors: Hiroki Nakae, Haruka Uesaka, Atsuhiko Minekawa, Takeshi Kurono
  • Patent number: 6892141
    Abstract: A primer design system in which DNA nucleotide sequences are obtained from a database comprising a plurality of different DNA nucleotide sequences, and the nucleotide sequences of primers capable of hybridizing specifically to the DNA thus obtained are determined. A plurality of primers capable of hybridizing specifically to mutually different DNAs can be efficiently designed.
    Type: Grant
    Filed: March 17, 2000
    Date of Patent: May 10, 2005
    Assignee: Hitachi, Ltd.
    Inventors: Hiroki Nakae, Sigeo Ihara
  • Publication number: 20030097223
    Abstract: A primer design system in which DNA nucleotide sequences are obtained from a database comprising a plurality of different DNA nucleotide sequences, and the nucleotide sequences of primers capable of hybridizing specifically to the exons predicted from the DNAs thus obtained are determined. A plurality of primers are simultaneously designed by using each of the predicted exons as a template. In addition, specificity evaluation is conducted for the exons, the primers and the primer pairs.
    Type: Application
    Filed: August 20, 2002
    Publication date: May 22, 2003
    Applicant: Hitachi, Ltd.
    Inventors: Hiroki Nakae, Sigeo Ihara