Abstract: A cell cluster from which unwanted components are removed by efficiently washing the cell cluster is collected. Provided is a cell washing method including a centrifuging step (S1) of collecting a cell suspension in a centrifuge container having a washing fluid discharge port opposing a bottom part and centrifugally separating cells at the bottom part by rotating in such a manner that the bottom part faces outward in the radial direction; a supernatant ejecting step (S2) of ejecting a supernatant centrifugally separated in the centrifuging step (S1); and a washing step (S3) of discharging the washing fluid from the discharge port while rotating the centrifuge container while ejecting the supernatant in the supernatant ejecting step (S2).

Abstract: A biological tissue is decomposed in a short time, and cells are obtained at a high yield without interfering with the health of the cells obtained through the decomposition. There is provided a method for decomposing biological tissue which decomposes a biological tissue under an action of a digestive enzyme, wherein a digestive juice containing the digestive enzyme is additionally supplied with the digestive enzyme either continuously or stepwisely over a predetermined time within a period from start of the decomposition until termination of the decomposition while the biological tissue is being immersed in the digestive juice.

Abstract: A biological tissue is decomposed in a short time, and cells are obtained at a high yield without interfering with the health of cells obtained through the decomposition. There is provided a method for decomposing biological tissue which decomposes a biological tissue under an action of a digestive enzyme, comprising: a decomposition starting step S32 of starting the decomposition of the biological tissue by immersing the biological tissue in a digestive juice having the digestive enzyme mixed in a solvent; and an enzyme concentration reduction step S31 of reducing a concentration of the digestive enzyme in the digestive juice at a predetermined timing after the start of the decomposition.