Abstract: A method for producing a brain organoid having an aggregated tau protein including (a) culturing pluripotent stem cells in the presence of a SMAD inhibitor to form an embryoid body, (b) embedding the embryoid body in an extracellular matrix and three-dimensionally culturing the embryoid body in the presence of a SMAD inhibitor and a GSK3? inhibitor to form an organoid that includes neural precursor cells, (c) extracting the organoid from the extracellular matrix and suspension-culturing the organoid in the presence of LIF to form a brain organoid, (d) forcing the brain organoid to express a mutant MAPT gene, and (e) further suspension-culturing the brain organoid after (d) to obtain a brain organoid having an aggregated tau protein.

Abstract: A production method for a cerebral organoid having amyloid plaques is provided, the method including a step (a) of forming, in the presence of a SMAD inhibitor, an embryoid body from a pluripotent stem cell having a mutation in an Alzheimer's disease-related gene; a step (b) of embedding the embryoid body after the step (a) in an extracellular matrix and three-dimensionally culturing the embedded embryoid body in the presence of a SMAD inhibitor and a glycogen synthase kinase 3? (GSK3?) inhibitor to form an organoid; and a step (c) of removing the organoid after the step (b) from the extracellular matrix and subjecting the removed organoid to stirring culture in a medium, where at least a part of the step (c) is carried out in the presence of leukemia inhibitory factor (LIF).

Abstract: To provide a method for forwarding a call which enables a connection between a called cellular phone and an IP phone, to which the incoming call is to be forwarded, by wireless communication, a method is provided for forwarding a call from the cellular phone to the IP phone in a communication system in which speech communication is carried out on an Internet Protocol network, comprising a first step in which during a call or a speech communication to the called cellular phone from a calling IP phone, a called person operates the called IP phone, to which the incoming call is to be forwarded, whereby a wireless communication is carried out between the called IP phone and the called cellular phone, and a second step in which after the first step, a function built in the called IP phone automatically performs communication with the calling IP phone, so that the incoming call to the called cellular phone is wirelessly forwarded to the called IP phone.