Abstract: When bacteria growth is determined in the related art using an ATP method, the bacteria growth is determined, based on an increase or a decrease in live bacteria ATP with the lapse of a culture time. Accordingly, it is necessary to measure the live bacteria ATP multiple times while antimicrobial susceptibility culture is carried out. It takes time and labor in sample preparation for each measurement, and it is difficult to quickly obtain an antimicrobial susceptibility result. Therefore, according to the present invention, the presence or absence of antimicrobial susceptibility of bacteria is determined, based on an ATP luminescence amount derived from dead bacteria in a culture liquid.

Abstract: Provided is a antimicrobial susceptibility testing device, including: an ATP examination culture plate that includes a reaction vessel, a reagent holding parts for holding reagents to be supplied to the reaction vessel, and a culture solution holding part for holding a culture solution to be supplied to the reaction vessel, and has plural layers that can be joined and separated; a gas feeding path for feeding a gas into the ATP examination culture plate; a heater; an optical detection unit; and a determination unit for determining sensitivity of a bacterial strain contained in the culture solution to a drug based on a detection result of the optical detection unit, wherein when the plural layers of the ATP examination culture plate are joined, at least the culture solution holding part and the reaction vessel are in a sealed state while communicating with each other.

Abstract: A system for culturing a photosynthetic organism such as a microalga has a liquid storage vessel for storing a liquid that absorbs more light in a short-wavelength range than light in a long-wavelength range, a culture vessel for storing a culture solution containing a photosynthetic organism to be cultured and disposed in the liquid storage vessel, a light quantity measuring unit for measuring the quantity of light that the culture vessel receives and a liquid depth controlling unit for controlling the liquid depth from the surface of the light-absorbing solution to the culture vessel based on the measurement result of the light quantity measuring unit. The light quantity measuring unit measures quantities of light which the culture vessel receives separately for different wavelength ranges.

Abstract: Provided is a antimicrobial susceptibility testing device, including: an ATP examination culture plate that includes a reaction vessel, a reagent holding parts for holding reagents to be supplied to the reaction vessel, and a culture solution holding part for holding a culture solution to be supplied to the reaction vessel, and has plural layers that can be joined and separated; a gas feeding path for feeding a gas into the ATP examination culture plate; a heater; an optical detection unit; and a determination unit for determining sensitivity of a bacterial strain contained in the culture solution to a drug based on a detection result of the optical detection unit, wherein when the plural layers of the ATP examination culture plate are joined, at least the culture solution holding part and the reaction vessel are in a sealed state while communicating with each other.

Abstract: When bacteria growth is determined in the related art using an ATP method, the bacteria growth is determined, based on an increase or a decrease in live bacteria ATP with the lapse of a culture time. Accordingly, it is necessary to measure the live bacteria ATP multiple times while antimicrobial susceptibility culture is carried out. It takes time and labor in sample preparation for each measurement, and it is difficult to quickly obtain an antimicrobial susceptibility result. Therefore, according to the present invention, the presence or absence of antimicrobial susceptibility of bacteria is determined, based on an ATP luminescence amount derived from dead bacteria in a culture liquid.

Abstract: As one aspect of the present invention, a chemical substance production system includes a first liquid reaction system that generates a first chemical substance, a second liquid reaction system that generates a second chemical substance, and a first membrane that is provided between the first liquid reaction system and the second liquid reaction system, wherein the second liquid reaction system causes a chemical reaction of the first chemical substance transferred from the first liquid reaction system to the second liquid reaction system through the first membrane, thereby generating the second chemical substance. According to this, a fuel production system that efficiently removes contaminants occurring in an extraction step when producing fuel derived from an alga or the like is provided.

Abstract: A system for culturing a photosynthetic organism such as a microalga has a liquid storage vessel for storing a liquid that absorbs more light in a short-wavelength range than light in a long-wavelength range, a culture vessel for storing a culture solution containing a photosynthetic organism to be cultured and disposed in the liquid storage vessel, a light quantity measuring unit for measuring the quantity of light that the culture vessel receives and a liquid depth controlling unit for controlling the liquid depth from the surface of the light-absorbing solution to the culture vessel based on the measurement result of the light quantity measuring unit. The light quantity measuring unit measures quantities of light which the culture vessel receives separately for different wavelength ranges.

Abstract: An object of the present invention is to enable simpler operation in real time and culture while removing unnecessary cells from cultured cells for purification in analyzing, fractionating, and culturing the cells alive and to analyze and fractionate desired cells from the cultured cells to increase the purity, recovery rate, and viability of the cells. The present invention employs a cell-adhesive photocontrollable base material, wherein light irradiation causes the bond dissociation of a photolabile group comprising a coumarinylmethyl skeleton to produce the separation of a cell-adhesive material to leave a non-cell-adhesive material. As a result, cell images can be detected and analyzed to obtain the positional information of desired cells. Based on the positional information thus obtained, the cells can be analyzed and fractionated alive.

Abstract: The subject invention provides a disease-treating drug that uses hollow protein nanoparticles to specifically act on a target cell or tissue. The present invention allows a protein drug to be effectively capsulated in the particles. The invention also provides a therapeutic method using such a drug. The drug according to the present invention is capable of recognizing a specific cell, such as hepatocytes, and manufactured by fusing a disease-treating substance for a target cell (for example, interferon, hepatocyte growth factor etc.) with hollow nanoparticles of a particle-forming protein (for example, hepatitis B virus surface-antigen protein).

Abstract: The invention provides hollow nanoparticles of a protein with the ability to recognize specific cells such as the hepatocytes and to form particles (for example, hepatitis B virus surface-antigen protein), wherein the protein has a cysteine residue substituted to a different amino acid. The hollow nanoparticles have a stable particle structure and can be used to efficiently transfer substances to specific target cells or tissues. The invention also provides a drug using the hollow nanoparticles.

Abstract: The subject invention provides a disease-treating drug that uses hollow protein nanoparticles to specifically act on a target cell or tissue. The present invention allows a protein drug to be effectively capsulated in the particles. The invention also provides a therapeutic method using such a drug. The drug according to the present invention is capable of recognizing a specific cell, such as hepatocytes, and manufactured by fusing a disease-treating substance for a target cell (for example, interferon, hepatocyte growth factor etc.) with hollow nanoparticles of a particle-forming protein (for example, hepatitis B virus surface-antigen protein).

Abstract: A bacterium of the genus Bacillus is transformed by introducing a vector incorporating DNA encoding an antibody; the resulting transformant is cultivated in a medium to produce and accumulate the antibody in an active form in the culture liquid. According to this method, a recombinant antibody can be easily produced in an active form in large amounts, owing to success in secretory expression using a bacterium of the genus Bacillus.

Abstract: Disclosed is a novel recombinant human lyphotoxin (LT) mutein which combines with an antibody at an inactive site of an LT molecule and liberates the LT molecule easily from the antibody after incorporation of the mutein into tumor cells.The recombinant human LT mutein is prepared by introducing a polydeoxyribonucleic acid containing a nucleotide sequence coding for the particular amino acid sequence into a replicable vector, transforming a microorganism or a cell with the recombinant DNA thus obtained, and cultivating the resulting transformant to express genetic information of the polydeoxyribonucleic acid.The recombinant human LT mutein is useful for tumor-selective carcinostatics, and effective for increasing the actively of LT and reducing the side effects thereof.

Abstract: The present invention provides human B lymphoblastoid cell line AC-33, which is novel, and excellent in proliferativity and fusion capability, and works well as the parental line for obtaining a human monoclonal antibody-producing hybridoma. Said hybridoma possesses excellent proliferativity and stable antibody productivity, thus permitting efficient antibody production over a long period.