Abstract: Disclosed are compositions, methods, and kits for modifying DNA within cells as well as compositions and methods for modifying gene expression in a cell. In particular, the invention generally relates to compositions, methods, and kits for DNA editing using single-stranded DNA. Compositions and methods for modifying gene expression using artificial microRNAs (amiRNA) are also contemplated.

Abstract: Disclosed are compositions, methods, and kits for modifying DNA within cells as well as compositions and methods for modifying gene expression in a cell. In particular, the invention generally relates to compositions, methods, and kits for DNA editing using single-stranded DNA. Compositions and methods for modifying gene expression using artificial microRNAs (amiRNA) are also contemplated.

Abstract: Disclosed are compositions, methods, and kits for modifying DNA within cells as well as compositions and methods for modifying gene expression in a cell. In particular, the invention generally relates to compositions, methods, and kits for DNA editing using single-stranded DNA. Compositions and methods for modifying gene expression using artificial microRNAs (amiRNA) are also contemplated.

Abstract: A forging tool used to forge a workpiece in a cuboidal forging space, wherein (a): the forging space is formed when the bottom surface of the first die and the bottom surface of the second die are brought into contact with the contact surface of the third die, or (b): the forging space is formed when a first die contact surface provided in the first die and a second die contact surface provided in the second die are brought into contact with each other.

Abstract: A forging tool is to forge a workpiece in a cuboidal forging space, and wherein (a): the forging space is formed when the bottom surface of the first die and the bottom surface of the second die are brought into contact with the contact surface of the third die, or (b): the forging space is formed when a first die contact surface provided in the first die and a second die contact surface provided in the second die are brought into contact with each other.

Abstract: A Cu—Be alloy according to the present invention is a Co-containing Cu—Be alloy, in which the Co content is 0.005% to 0.12% by mass, and the number of Cu—Co-based compound particles having a particle size of 0.1 ?m or more that can be confirmed on a TEM image at a magnification of 20,000 is five or less in a field of view of 10 ?m×10 ?m. Furthermore, a method for producing a Cu—Be alloy according to the present invention includes a solution annealing treatment step of subjecting a Cu—Be alloy raw material containing 0.005% to 0.12% by mass of Co and 1.60% to 1.95% by mass of Be to solution annealing treatment to obtain a solution-annealed material.

Abstract: Disclosed are compositions, methods, and kits for modifying DNA within cells as well as compositions and methods for modifying gene expression in a cell. In particular, the invention generally relates to compositions, methods, and kits for DNA editing using single-stranded DNA. Compositions and methods for modifying gene expression using artificial microRNAs (amiRNA) are also contemplated.

Abstract: Disclosed are compositions, methods, and kits for modifying DNA within cells as well as compositions and methods for modifying gene expression in a cell. In particular, the invention generally relates to compositions, methods, and kits for DNA editing using single-stranded DNA. Compositions and methods for modifying gene expression using artificial microRNAs (amiRNA) are also contemplated.

Abstract: A forging method according to the present invention includes the steps of placing a work having a first shape which is a rectangular hexahedron in a work space of a forging die which has a rectangular opening, which is formed by rectangular plane wall portions, and which is provided with the work space to hold the work in a placement step and applying a plastic strain to the work by deforming the placed work into a second shape which is a rectangular hexahedron in a working step, with the placement step and the working step being performed at least two times.

Abstract: A method for producing a high-strength magnesium alloy material includes (a) a step of preparing a magnesium alloy workpiece having a top face and a side face; and (b) a step of applying a compressive load ?p (MPa) from the top face side of the workpiece and performing a uniaxial forging process on the workpiece. Step (b) is performed while suppressing deformation of the workpiece widening outward and under conditions including (i) ?p>?f (where ?f is the compressive breaking stress (MPa) of the workpiece); (ii) a plastic deformation rate is less than or equal to 10%, and (iii) a strain rate is less than or equal to 0.1/sec.

Abstract: A Cu—Be alloy according to the present invention is a Co-containing Cu—Be alloy, in which the Co content is 0.005% to 0.12% by mass, and the number of Cu—Co-based compound particles having a particle size of 0.1 ?m or more that can be confirmed on a TEM image at a magnification of 20,000 is five or less in a field of view of 10 ?m×10 ?m. Furthermore, a method for producing a Cu—Be alloy according to the present invention includes a solution annealing treatment step of subjecting a Cu—Be alloy raw material containing 0.005% to 0.12% by mass of Co and 1.60% to 1.95% by mass of Be to solution annealing treatment to obtain a solution-annealed material.

Abstract: A forging method according to the present invention is characterized by including the steps of placing a work W having a first shape which is a rectangular hexahedron in a work space 45 of a forging die 20 which has a rectangular opening, which is formed by rectangular plane wall portions, and which is provided with the work space 45 to hold the work in a placement step and applying a plastic strain to the work W by deforming the placed work W into a second shape which is a rectangular hexahedron in a working step, wherein the placement step and the working step are performed at least two times.

Abstract: A method for producing a high-strength magnesium alloy material includes (a) a step of preparing a magnesium alloy workpiece having a top face and a side face; and (b) a step of applying a compressive load ?p (MPa) from the top face side of the workpiece and performing a uniaxial forging process on the workpiece. Step (b) is performed while suppressing deformation of the workpiece widening outward and under conditions including (i) ?p>?f (where ?f is the compressive breaking stress (MPa) of the workpiece); (ii) a plastic deformation rate is less than or equal to 10%, and (iii) a strain rate is less than or equal to 0.1/sec.

Abstract: In the present invention, there is provided a fabrication method of a material having an ultrafine grained structure characterized by including a step of providing a metal or an alloy having a stacking fault energy no greater than 50 mJ/mm2 and a step of introducing deformation twins having a twin interval no greater than 200 nm into structures of the metal or the alloy by processing the metal or the alloy. Further, according to this method, a material having an ultrafine grained structure characterized in that twins are included in a crystal structure and the twins have a twin interval no greater than 200 nm.

Abstract: A slide photograph data creation system includes a digital camera for making high-magnification photographs of samples, a sample transporter for holding and transporting samples, a controller for controlling the camera and the sample transporter so as to provide overlapping photographs, a pasting information generator for recognizing the margin by which the photographs overlap and for generating pasting-together information, and a photograph file generator for storing in a single file a plurality of high-magnification photographs and the pasting-together information. The system facilitates the examination and photography of large numbers of samples, and enables discrimination of the three-dimensional structure of a sample. Since the photographic data is managed with a database, virtual slide photographs and their attributes information can be browsed over a network or the Internet.

Abstract: A slide photograph data creation system includes a digital camera for making high-magnification photographs of samples, a sample transporter for holding and transporting samples, a controller for controlling the camera and the sample transporter so as to provide overlapping photographs, a pasting information generator for recognizing the margin by which the photographs overlap and for generating pasting-together information, and a photograph file generator for storing in a single file a plurality of high-magnification photographs and the pasting-together information. The system facilitates the examination and photography of large numbers of samples, and enables discrimination of the three-dimensional structure of a sample. Since the photographic data is managed with a database, virtual slide photographs and their attributes information can be browsed over a network or the Internet.

Abstract: A method for obtaining information on the primary structure of peptides comprising fragmenting the proteins, which enables automation of obtaining information on the primary structure of a peptide sample; a device for use in the method; and an automatic analysis system on the primary structure of the peptides. The method for obtaining information on the primary structure of peptides comprising the steps of: denaturing, reducing, and alkylating a protein; immobilizing the reduced alkylated protein obtained on a hydrophobic microparticulate carrier; chemically or enzymatically fragmenting the immobilized protein obtained; and obtaining information on the primary structure of the peptides fragmented.